Open Access

Effects of ketanserin on experimental colitis in mice and macrophage function

  • Authors:
    • Junhua Xiao
    • Limei Shao
    • Jiaqing Shen
    • Weiliang Jiang
    • Yun Feng
    • Ping Zheng
    • Fei Liu
  • View Affiliations

  • Published online on: February 10, 2016     https://doi.org/10.3892/ijmm.2016.2486
  • Pages: 659-668
  • Copyright: © Xiao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Ketanserin is a selective 5-hydroxytryptamine (serotonin)-2A receptor (5-HT2AR) antagonist. Studies have suggested that ketanserin exerts anti-inflammatory effects independent of the baroreflex; however, the mechanisms involved remain unclear. Thus, in the present study, we aimed to evaluate the effects of ketanserin in colitis and the possible underlying mechanisms. The expression of 5-HT2AR was assessed in the colon tissues of patients with inflammatory bowel disease (IBD) and in mice with dextran sodium sulfate (DSS)-induced colitis. The therapeutic potential of ketanserin was investigated in the mice with colitis. In the colon tissue samples from the patients with IBD, a high expression level of 5-HT2AR was observed. Treatment with ketanserin attenuated the progression of experimental colitis in the mice, as indicated by body weight assessment, colon length, histological scores and cytokine release. The colonic macrophages from the ketanserin-treated mice with colitis exhibited a decreased production of inflammatory cytokines, with M2 polarization and impaired migration. The knockdown of 5-HT2AR using siRNA partly abolished the inhibitory effects of ketanserin on the release of pro-inflammatory cytokines in bone marrow derived-macrophages (BMDMs), thus demonstrating that the inhibitory effects of ketanserin on the production of inflammatory cytokines are partly dependent on 5-HT2AR. Ketanserin also inhibited the activation of nuclear factor-κB (NF-κB) in BMDMs. In conclusion, the findings of the present study demonstrate that ketanserin alleviates colitis. Its anti-inflammatory effects may be due to the promotion of the anti-inflammatory function of macrophages through 5-HT2AR/NF-κB.

Introduction

Inflammatory bowel disease (IBD) is a group of chronic inflammatory conditions that affect the gastrointestinal tract and includes two main clinical forms: Crohn's disease (CD) and ulcerative colitis (UC). Although the precise etiology of IBD is not yet clearly understood, it has been suggested that an abnormal immune response against intestinal microbes plays a role in the pathogenesis of colitis (1).

5-Hydroxytryptamine (serotonin; 5-HT) modulates intestinal inflammation in the pathogenesis of colitis. Changes in the levels of 5-HT have been observed in experimental models of colitis and in patients with IBD (24). The knockdown 5-HT expression in tryptophan hydroxylase 1-deficient (TPH1−/−) mice, has been shown to lead to a delayed onset and to reduce the severity of dextran sodium sulfate (DSS)-induced colitis (5). Mucosal changes in IBD are characterized by ulcerative lesions with the prominent infiltration of immune cells and alterations in serotonin-producing enterochromaffin cells (6). 5-HT promotes the production of pro-inflammatory cytokines from macrophages (7), and nuclear factor-κB (NF-κB) signaling plays a critical role in the 5-HT-mediated activation of immune cells (8). However, the precise functions of 5-HT in intestinal inflammation are not yet fully understood. This is partly due to the fact that a wide variety of 5-HT receptors are expressed in the gut; 5-HT1A, 5-HT2A, 5-HT2B, 5-HT3, 5-HT4 and 5-HT7 receptors are expressed in the intestines and some are known to have varying, and even contrasting roles.

Ketanserin is a selective 5-HT2A receptor (5-HT2AR) antag-antagonist with minor activity on the α1 adrenergic receptor (9). Previous studies have suggested that ketanserin exerts anti-inflammatory effects independent of the baroreflex (10,11). Ketanserin has been shown to inhibit the expression of inducible nitric oxide (NO) synthase (iNOS), NO, tumor necrosis factor-α (TNF-α), and interleukin (IL)-6 in lipopolysaccharide (LPS)-stimulated RAW264.7 cells (12). Moreover, the administration of ketanserin has been shown to decrease the expression of iNOS following endotoxic shock in mice (13). iNOS is primarily produced by macrophages. Macrophages participate in the immune response to the microenvironment in a polarized manner (14). Macrophages are often divided into 2 functional groups: 'inflammatory' M1 macrophages (high IL-12 and iNOS expression) and 'wound-healing' M2 macrophages (low IL-12 expression, and high IL-10 expression) (15). M1 macrophages mainly produce inflammatory cytokines (TNF-α and IL-12) and iNOS. The production of these cytokines promotes naïve CD4+ T cells to differentiate into Th1 and Th17 cells (16). M2 macrophages produce the anti-inflammatory cytokine, IL-10, and are efficient at recruiting regulatory T cells that promote tolerance to local immune responses (16). In this study, we examined the hypothesis that ketanserin may attenuate colitis by modulating the function of macrophages, thus alleviating colitis.

Materials and methods

Human tissue samples

Intestinal specimens were obtained from 15 patients with IBD. Normal tissue specimens were obtained from patients receiving colonoscopy for colorectal cancer screening. The tissues were frozen in liquid nitrogen and stored at −80°C for reverse transcription-quantitative PCR (RT-qPCR) analyses, or were embedded in optimum cutting temperature (OCT) compound for immunofluorescence staining. All studies involving samples obtained from human participants were approved by the Ethics Committee of the Humanitas Clinical and Research Center of Shanghai East Hospital, Shanghai, China. Written informed consent was obtained from each patient or their family before initiating the study protocol.

Mice

A total of 30 C57BL/6 male mice (8 to 10 weeks old, weighing 20 g) were purchased from Slaccas Laboratory Animal Co., Ltd. (Shanghai, China) and all experiments were performed under specific pathogen-free conditions at the Animal Resources facilities of Shanghai East Hospital. All animal experiments were performed following approval by the Animal Care and Use Committee of Shanghai East Hospital.

Induction of colitis by DSS

The mice were randomly divided into 3 groups as follows: the control group, DSS treatment group and the DSS plus ketanserin treatment group (n=10 in each group). The mice were administered 4% (wt/vol) DSS (MP Biomedicals, Solon, OH, USA) dissolved in their drinking water for 7 days to induce acute experimental colitis. Ketanserin (10 mg/kg; Janssen Pharmaceuticals, Beijing, China) or phosphate-buffered saline (PBS; vehicle control; administered to the mice in the DSS treatment group) were administered intra-peritoneally once daily for 3 days when the administration of DSS began. The weight of the mice was recorded daily using an electronic weighing system (Practum 612-1CN; Sartorius AG, Goettingen, Germany). On day 7, the mice were sacrificed by an intraperitoneal injection of 4% sodium pentobarbital and the colons were obtained for the measurement of colon length. For histological analysis, the colons were excised and fixed in 4% paraformaldehyde. Subsequentoy, 1 cm of the distal colon of each mouse was paraffin-embedded. The paraffin-embedded sections were cut (4-μM-thick) using a microtome and stained with hematoxylin and eosin. For gene and protein expression analyses, the colons were immediately frozen in liquid N2 and stored at −80°C.

RT-qPCR

Total RNA was extracted from the colon tissues or lamina propria macrophages (LP-macrophages) and bone marrow derived-macrophages (BMDMs) using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's instructions. Complementary DNA (cDNA) was synthesized using the PrimeScript™ RT reagent kit (Takara, Shiga, Japan). Messenger RNA (mRNA) transcripts were analyzed by quantitative PCR using SYBR® Premix Ex Taq™ (Takara) with an Applied Biosystems StepOne/StepOnePlus Real-Time PCR System. Gene expression was normalized to the expression of glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and the relative expression levels were quantified using the 2−ΔΔCT method. The primer pairs used are listed in Table I.

Table I

Sequences of primers used for RT-qPCR.

Table I

Sequences of primers used for RT-qPCR.

Gene productForward primerReverse primer
mTNF-α 5′-gtcgtagcaaaccaccaagtg-3′ 5′-cagatttgtgttggtccttc-3′
mIL-1β 5′-atctcgcagcagcacatcaac-3′ 5′-cacgggaaagacacaggtagc-3′
mIL-6 5′-acaagtcggaggcttaattacacat-3′ 5′-ttgccattgcacaactcttttc-3′
mIL-10 5′-gctcttactgactggcatgag-3′ 5′-cgcagctctaggagcatgtg-3′
mIL-12 p40 5′-agacatggagtcataggctctg-3′ 5′-ccattttccttcttgtggagca-3′
miNOS 5′-gccaccaacaatggcaaca-3′ 5′-cgtaccggatgagctgtgaatt-3′
mKC 5′-cttgaaggtgttgccctcag-3′ 5′-tggggacaccttttagcatc-3″
mCXCL12 5′-aaaccagtcagcctgagctac-3′ 5′-aagggcacagtttggagtgtt-3′
mCD206 5′-ttcggtggactgtggacgagca-3′ 5′-ataagccacctgccactccggt-3′
mCD32 5′-aatcctgccgttcctactgatc-3′ 5′-gtgtcaccgtgtcttccttgag-3′
m5-HT2A− 5′-cgactcgaggctctacccta-3′ 5′-ttcgcaggagaggttggttc-3′
h5-HT2AR 5′-tgaagtcagcaaaacagaaacca-3′ 5′-gactcgctgcatctctcaca-3′

[i] TNF-α, tumor necrosis factor-α; IL-1β, interleukin-1β; iNOS, inducible nitric oxide synthase; KC, keratinocyte-derived chemokine; 5-HT2A-R, 5-hydroxytryptamine-2A receptor.

Western blot analysis

Colon proteins were extracted using RIPA buffer supplemented with protease inhibitors (Shanghai Shenggong Co., Ltd., Shanghai, China). A total of 40 μg protein was separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and the gels were then electrotransferred onto nitrocellulose filter membranes (NC; Whatman, Alameda, CA, USA). The membranes were incubated with antibodies against total NF-κB (p65; Cat. no. 8424; 1:1,000), phosphorylated (p-)NF-κB (p65; Cat. no. 3033; 1:1,000), or GAPDH (Cat. no. 8884; 1:1,000) (all from Cell Signaling Technology, Beverly, MA, USA) overnight at 4°C. The membranes were then incubated with an IRDye 800CW-conjugated secondary antibody (Rockland, Hamburg, Germany) for 1 h at room temperature. Images were acquired using an Odyssey infrared imaging system (LI-COR Biosciences, Inc., Lincoln, NE, USA).

Flow cytometric analysis

Briefly, the colons were longitudinally cut and washed in PBS containing 1% fetal bovine serum and 1 mM DTT for 10 min at room temperature 3 times. Subsequently, the colon tissues were digested in complete RPMI-1640 medium (Invitrogen) supplemented with 0.5 mg/ml collagenase type VIII (Sigma-Aldrich, St. Louis, MO, USA), 20 mg/ml DNase I (Roche Diagnostics, Mannheim, Germany) for 60 min at 37°C in a shaking water bath. The cell suspension was passed through a 100–70 μm filter and then resuspended in 1.077 g/cm3 iso-osmotic metrizamide medium (Accurate Chemical & Scientific Corp., Westbury, NY, USA). Following centrifugation at 1,500 × g for 15 min at room temperature, the low-density fraction was collected. For FACS analysis of the colon samples, the cells were stained with antibodies to CD45 (17-0451), CD11c (12-0114), MHC-II (56-5321), F4/80 (15-4801), CD11b (11-0112), Ly-6G (Gr-1; 53-5931) (all from eBioscience, San Diego, CA, USA) and CD206 (141711) (from BioLegend, San Diego, CA, USA). The influx of neutrophils, dendritic cells (DCs) and macrophages was determined by the frequency of macrophages, neutrophils and DCs in CD45+ cells in the LP of colons.

Isolation and stimulation of murine macrophages

To isolate the LP-macrophages from the lamina propria of the mouse colons, the colons were washed with PBS and cut into small sections. The latter were then incubated with 5 mM EDTA and 3% FCS in Ca2+- and Mg2+-free Hanks balanced salt solution for 30 min at 37°C with stirring. The small sections were collected and were then digested with RPMI-1640 containing 5% FCS, 1 mg/ml collagenase type IV and 0.1 mg/ml DNase for 1 h at 37°C. The liberated cells were collected through a stainless steel sieve. Following centrifugation (500 × g, 4°C) and washing with PBS 3 times, the intestinal macrophages were enriched by positive selection with anti-CD11b Dynabeads following the manufacturer's instructions (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany). The cells obtained were tested for the expression of macrophage markers by flow cytometry (CD45+CD11b+CD11cF4/80+); cells with a purity >85% were used in the experiments.

LP-macrophages (5×104) were cultured in complete RPMI-1640 medium and treated with 100 ng/ml LPS for 6 h. Supernatants were collected for the measurement of cytokine levels and the attached cells were lysed for RNA extraction. Briefly, the levels of the cytokines, IL-1β, IL-6, IL-10 and TNF-α, were evaluated in the supernatant from the macrophage cultures by enzyme-linked immunosorbent assay (ELISA), following the manufacturer's instructions (R&D Systems, Inc., Minneapolis, MN, USA).

Migration and phagocytosis of LP-macrophages

The migration of the LP-macrophages induced by C-X-C motif chemokine 12 [CXCL12, also known as stromal cell-derived factor 1 (SDF-1)] was assessed using a 48-well microchemotaxis chamber (Neuro Probe, Gaithersburg, MD, USA). CXCL12 (50 nM; 250-20B, PeproTech, Rocky Hill, NJ, USA) was placed in wells (30 μl) of the lower compartment, and LP-macrophages (50 μl of a 5×10*4/ml suspension) were seeded in the wells of the upper compartment. The chamber was incubated in a humidified environment at 37°C with 5% CO2 for 4 h. The membrane was removed, fixed in methanol, and stained with crystal violet solution for 30 min followed by destaining with water. The cells that had migrated across the membrane were counted using a microscope (Leica TCS SP8 CARS confocal microscope, Leica Microsystems GmbH, Wetzlar, Germany). The chemotaxis index was calculated as the ratio of the number of cells that had migrated towards the chemoattractant divided by the number of cells that had migrated towards the medium.

The LP-macrophages (5×10*4) were infected with E. coli at an MOI of 1:10 for 1 h in complete RPMI-1640 medium without antibiotics, and then incubated in complete medium containing 40 μg/ml gentamycin for 120 min. The cells were then lysed and seeded on LB agar plates. Following overnight incubation at 37°C, bacterial colonies (CFUs) were counted as a measure of intracellular bacteria.

Immunofluorescence staining

For the human tissues, 4-μm-thick frozen sections of intestinal specimens were fixed in cold acetone for 10 min at −20°C and blocked with 5% BSA for 1 h at room temperature, then incubated with a primary rabbit anti-human 5-HT2AR antibody (ab66049; rabbit polyclonal to 5-HT2AR; reacts to mouse, rat, human; Abcam, Cambridge, UK; 1:500) overnight at 4°C. Mouse anti-human CD68 antibody (MCA5709; mouse anti-human CD68, monoclonal antibody; AbD Serotec, Kidlington, UK; 1:500) (overnight at 4°C) was subsequently used to detect the macrophages. For the murine colon tissues, 5-HT2AR expression was detected by overnight incubation at 4°C with rabbit anti-mouse 5-HT2AR antibody (ab66049; rabbit polyclonal to 5-HT2AR; reacts to mouse, rat, human; Abcam; 1:300). Subsquently, rat anti-mouse CD68 antibody (MCA1957GA; rat anti-mouse CD68, monoclonal antibody; AbD Serotec; 1:500) was used overnight at 4°C. For both analyses, Alexa-Fluor 488- and Alexa-Fluor 555-conjugated antibodies [goat anti-rabbit IgG (ab150077, goat anti-rat IgG (ab150158) and goat anti-mouse IgG (ab150118); all from Abcam, Cambridge, UK; 1:1,000; 1 h at room temperature] were used as secondary antibodies, followed by incubation with 1 μg/ml DAPI (20 min, room temperature). The sections were finally visualized under a confocal microscope (Olympus, Tokyo, Japan). Images were captured using FluoView software.

Transfection with small interfering RNA (siRNA)

siRNA targeting the 5-HT2AR (Genepharm Biotech, Shanghai, China) was synthesized (GACAACUGUCGUGAUUAUUTT) and control siRNA (UUCUCCGAACGUGUCACGUTT) was also used. BMDMs were isolated from bone marrow cells obtained from the mice. Three C57BL/6 male mice (8 to 10 weeks old, weighing 20 g) were sacrificed as described for the mice in the other experiments, and bone marrow cells were isolated from femurs and cultured with RPMI-1640 supplemented with 10% FBS, 1% penicillin and streptomycin (Invitrogen) and 10% L929 conditioned medium. The culture fluid was exchanged for fresh culture medium every 4 days. Under these conditions, adherent macrophage monolayers were obtained within 8–10 days. The BMDMs were then transfected with the siRNAs as previously described (17). Following transfection, the BMDMs (1×106/ml) were pre-incubated with ketanserin (10 μM) for 10 min and then stimulated with LPS (100 ng/ml) and 5-HT (100 nmol/l) for 12 h. RNA was then extracted for PCR.

Statistical analysis

The statistical significance of the differences between the treatment and control groups was determined using a Student's t-test. Data were analyzed with one-way analysis of variance (ANOVA), followed by the Student's t-test for experiments involving only 2 groups, and Dunnett's t-test for experiments involving >2 groups. All data are expressed as the means ± standard deviation (SD). Statistical significance was set at P<0.05.

Results

5-HT2AR expression is elevated in both patients with IBD and in mice with experimental colitis, and is specifically enhanced in macrophages

We first examined the expression of 5-HT2AR in patients with IBD and in mice with DSS-induced experimental colitis. The 5-HT2AR mRNA level was increased in the patients with IBD (Fig. 1A) compared to the normal tissue specimens, and 5-HT2AR protein expression was also elevated in the patients with IBD, as detected by immunofluorescence staining (Fig. 1B). 5-HT2AR is known to be expressed on the surface of lymphocytes, natural killer (NK) cells and monocytes/macrophages/DCs (18,19). We observed a specific co-localization of 5-HT2AR with the CD68 macro-with the CD68 macrophage marker (Fig. 1B), reflecting that 5-HT2AR expression is enhanced in macrophages. The 5-HT2AR expression level was also upregulated in the mice with DSS-induced experimental colitis (Fig. 1C and D) and was co-localized with CD68 postivity (Fig. 1E). These data suggest that the synthesis of 5-HT2AR is induced in the inflamed colon and that it is mostly expressed in macrophages.

The administration of ketanserin attenuates the development of DSS-induced acute experimental colitis in mice

To investigate whether ketanserin reduces susceptibility to colitis, we induced colitis in mice using DSS. The mice with DSS-induced colitis exhibited a continuous decrease in body weight from day 4 to day 7 and shortened colon lengths. By contrast, the administration of ketanserin during the induction of colitis significantly prevented the decrease in body weight (Fig. 2A) and colon shortening (Fig. 2B). A histological examination of the colons of the mice with DSS-induced colitis revealed severe inflammation with ulcerative lesions, loss of crypts and the infiltration of inflammatory cells, whereas treatment with ketanserin alleviated these histological changes and damage to the colon, characterized by a decrease in the loss of architecture, fewer ulcerative lesions, and a decrease in inflammatory cell infiltration into the inflamed mucosa (Fig. 2C and D). Our data therefore suggest that ketanserin exerts a potent therapeutic effect, ameliorating DSS-induced colitis.

DSS is directly toxic to the colonic epithelium and triggers inflammation in the gut with high levels of inflammatory mediators, such as iNOS, IL-1β and IL-6 (20,21). We thus evaluated the effects of ketanserin on the production of inflammatory mediators in the colonic mucosa by RT-qPCR. Compared with the vehicle-treated mice with colitis (DSS group), the ketanserin-treated mice exhibited significantly lower mRNA levels of keratinocyte-derived chemokine (KC; a major chemoattractant for neutrophils), iNOS, TNF-α, IL-1β and IL-6 (Fig. 2E). Moreover, the transcript levels of IL-10 were found to be higher in the ketanserin-treated mice. The levels of CXCL12, the major chemoattractant for DCs, remained unaltered.

Ketanserin reduces the infiltration of neutrophils and macrophages into the intestinal mucosa in colitis

Innate immune cells are the major sources of inflammatory mediators in DSS-induced colitis (22,23). We thus investigated whether the effects of ketanserin alter the infiltration of innate immune cells, thus influencing the inflammatory response in the colon. In the mice with DSS-induced colitis, the percentage of neutrophils, macrophages and DCs in the CD45+ cells was significantly increased in the colonic lamina propria on day 7 compared with the control mice (Fig. 3A). Consistently, treatment with ketanserin resulted in a decrease in the influx of neutrophils and macrophages into the inflamed colons of the mice (Fig. 3B). Minor differences in the relative percentage of DCs in the CD4+ T cell population were observed. We also found that the colonic macrophages in the ketanserin-treated group expressed higher levels of CD206, a marker of M2 macrophages, compared to the vehicle-treated mice with colitis (DSS group; Fig. 3A and C).

Ketanserin inhibits the migration of LP-macrophages without affecting the ability of bacterial phagocytosis

As the ketanserin-treated mice exhibited a reduced influx of macrophages into the colonic lamina propria, we then determined whether ketanserin suppresses the chemotaxis of macrophages. As CXCL12 is a critical regulator of macrophage migration (24,25), the migration of LP-macrophages through an 8-μm filter towards CXCL12 (50 nM) placed in the lower chamber was examined. The chemotaxis index indicated that ketanserin significantly reduced the migration of macrophages towards CXCL12 (Fig. 4A and B).

It has been shown that 5-HT modulates the activity of the phagocytosis of bacteria by macrophages through 5-HT receptors (26). Thus, to determine whether ketanserin affects the phagocytic ability of macrophages, LP-macrophages were infected with E. coli and viable intracellular bacteria CFUs were counted in order to evaluate bacterial phagocytosis. We found that the ability of the LP-macrophages to phagocytize E. coli in the ketanserin-treated mice with colitis did not differ from that in the vehicle-treated mice with colitis (DSS group; Fig. 4C).

Ketanserin inhibits the expression of pro-inflammatory mediators and influences the polarization of LP-macrophages

We then evaluated the release of cytokines in the supernatants of LP-macrophages obtained from the inflamed colon. In line with our hypothesis, the secretion of TNF-α, IL-1β and IL-6 was significantly decreased in the ketanserin-treated mice (Fig. 5A–C). In addition, the ketanserin-treated mice produced higher amounts of IL-10 (Fig. 5D).

Since the LP-macrophages from the ketanserin-treated mice with colitis exhibited a high level of CD206, a marker of M2 macrophages (as shown by FACS analysis), we therefore measured the mRNA levels of iNOS, CD32 and IL-12 p40, as M1 polarization markers, and the levels of CD206 and IL-10, as M2 polarization markers in the LP-macrophages in all 3 groups. Of note, the LP-macrophages from the ketanserin-treated mice exhibited a notably decreased expression of iNOS, CD32 and IL-12 p40 (Fig. 5E), but elevated levels of CD206 and IL-10 (Fig. 5F). Taken together, these data indicate that ketanserin modulates LP-macrophage function by reducing the production of pro-inflammatory cytokines and promoting M2 as opposed to M1 polarization.

Ketanserin inhibits the expression of pro-inflammatory cytokines in BMDMs through 5-HT2AR/NF-κB

In order to determine whether 5-HT2AR plays a role in the effects of ketanserin, we used 5-HT2AR siRNA to downregulate the expression of 5-HT2AR in BMDMs. The LPS/5HT-induced expression of the pro-inflammatory cytokines, IL-1β (Fig. 6A), TNF-α (Fig. 6B), IL-6 (Fig. 6C) and iNOS (Fig. 6D), was inhibited by treatment with ketanserin in the BMDMs. The knockdown of 5-HT2AR by siRNA partly abolished the inhibitory effects of ketanserin on the expression of these pro-inflammatory cytokines in the BMDMs stimulated with LPS plus 5-HT (Fig. 6). In a previous study, it was suggested that the anti-inflammatory effects of ketanserin are partly dependent on the arterial baroreflex (13). In the present study, we found that ketanserin decreased the levels of inflammatory cytokines in BMDMs, suggesting that the anti-inflammatory effects of ketanserin are not entirely dependent on the baroreflex. Moreover, these effects were dimished by transfection with siRNA targeting 5-HT2AR, thus demonstrating that the inhibitory effects of ketanserin are partly-dependent on 5-HT2AR.

NF-κB is a critical transcription factor for the inflammatory response (27). It functions as a pro-inflammatory factor and participates in the pathophysiology of IBD (28). Thus, in order to elucidate the mechanisms responsible for the anti-inflammatory effects of ketanserin, we examined its effects on the activation of the NF-κB pathway in BMDMS. Changes in the levels of p-NF-κB p65 in the BMDMs treated with or without ketanserin were evaluated by western blot analysis. As demonstrated in Fig. 6E and F, ketanserin markedly decreased the protein level of p-p65 compared with the BMDMs not treated with ketanserin. These data indicate that 5-HT2AR/NF-κB may play a role in the anti-inflammatory effects of ketanserin in macrophages.

Discussion

The unresolved inflammatory state in the intestinal tract, which was caused by failing to switch from a pro-inflammatory response to an inflammation-resolving anti-inflammatory response, is a characteristic of IBD (29). Both macrophages and DCs actively promote the transition from an inflammatory state to a homeostatic state following immune system activation, and unresolved inflammation is associated with IBD (30). Macrophages are a highly heterogeneous population of cells with a continuum of activation states (31). Evidence indicates that of the macrophages in the lamina propria, the pro-inflammatory M1 subtype is more prominent in patients with CD and less prominent in patients with UC (32).

In the present study, we found that 5-HT2AR, which was elevated in colitis, was specifically enhanced in CD68+ macrophages. Treatment with ketanserin, a selective 5-HT2AR antagonist, significantly decreased the DSS-induced production of iNOS and pro-inflammatory cytokines (TNF-α and IL-6) in the colons of mice with experimental colitis, with a marked reduction in the infiltration of neutrophils and macrophages in the intestinal mucosa, without a significant difference in the frequency of DCs. Furthermore, ketanserin inhibited the production of iNOS, CD32 and IL-12 in the LP-macrophages upon challenge with LPS, whereas the production of IL-10 and CD206 increased. This indicates that ketanserin may influence macrophages by promoting their anti-inflammatory function and alterating the polarization from M1 to M2.

The change in the cytokine profile in the ketanserin-treated macrophages is consistent with that of other studies. Ahonen et al observed significantly higher levels of IL-1 and IL-6 in LPS-stimulated macrophages following incubation with 5-HT in their study (33). In the study by Liu et al, ketanserin markedly reduced the expression of iNOS, TNF-α and IL-6, as well as that of reactive oxygen species in RAW264.7 cells upon challenge with LPS (12).

The murine chemokines, KC and CXCL12, are required for neutrophil and macrophage recruitment, respectively to the sites of inflammation. Our results indicated that ketanserin induced a reduction in the KC level along with a considerable decrease in the number of macrophages in the colonic mucosa, suggesting that the efficacy of ketanserin may be related to a lower neutrophil and macrophage influx to the site of injury. In a previous study, there was also a significant decrease in macrophage infiltration in TPH1−/− mice with DSS-induced colitis (5). In addition, we demonstrated that ketanserin plays a direct inhibit role in the migration of macrophages, without affecting macrophage phagocytosis.

We also examined 5-HT2AR expression in colitis and found that macrophages expressed high levels of 5-HT2AR in the inflamed colon. 5-HT2AR expression has been also shown to be increased in other inflammatory conditions, such as allergic contact dermatitis (34). Furthermore, we demonstrated that 5-HT2AR siRNA partly reversed the anti-inflammatory effects of ketanserin in BMDMs, thus demonstrating that the inhibitory effects of ketanserin are partly-dependent on 5-HT2AR. In addition, we demonstrated that ketanserin inhibits NF-κB activation in BMDMs. These findings reveal an essential role of 5-HT2AR/NF-κB in the effects of ketanserin on the release of cytokines.

In conclusion, the results presented herein demonstrate that treatment with ketanserin attenuated the progression of DSS-induced colitis in mice. The mechanisms responsible for these effects include the inhibition of the release of inflammatory cytokines and the inhibition of the migration of macrophages, but not of their phagocytic ability. Ketanserin also promoted the M2 polarization of colonic macrophages. Moreover, 5-HT2AR/NF-κB may play a role in the therapeutic effects of ketanserin in colitis.

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March-2016
Volume 37 Issue 3

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Spandidos Publications style
Xiao J, Shao L, Shen J, Jiang W, Feng Y, Zheng P and Liu F: Effects of ketanserin on experimental colitis in mice and macrophage function. Int J Mol Med 37: 659-668, 2016
APA
Xiao, J., Shao, L., Shen, J., Jiang, W., Feng, Y., Zheng, P., & Liu, F. (2016). Effects of ketanserin on experimental colitis in mice and macrophage function. International Journal of Molecular Medicine, 37, 659-668. https://doi.org/10.3892/ijmm.2016.2486
MLA
Xiao, J., Shao, L., Shen, J., Jiang, W., Feng, Y., Zheng, P., Liu, F."Effects of ketanserin on experimental colitis in mice and macrophage function". International Journal of Molecular Medicine 37.3 (2016): 659-668.
Chicago
Xiao, J., Shao, L., Shen, J., Jiang, W., Feng, Y., Zheng, P., Liu, F."Effects of ketanserin on experimental colitis in mice and macrophage function". International Journal of Molecular Medicine 37, no. 3 (2016): 659-668. https://doi.org/10.3892/ijmm.2016.2486