Role of Alix in miRNA packaging during extracellular vesicle biogenesis

Iavello,Alessandra; Frech,Valeska  S.L.; Gai,Chiara; Deregibus,Maria  Chiara; Quesenberry,Peter  J.; Camussi,Giovanni

doi:10.3892/ijmm.2016.2488

Role of Alix in miRNA packaging during extracellular vesicle biogenesis

Authors:
- Alessandra Iavello
- Valeska S.L. Frech
- Chiara Gai
- Maria Chiara Deregibus
- Peter J. Quesenberry
- Giovanni Camussi
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Affiliations: Department of Medical Sciences and Molecular Biotechnology Center, University of Torino, 10126 Torino, Italy, Department of Medicine, The Warren Alpert Medical School of Brown University, Providence, RI 02903, USA
Published online on: February 12, 2016 https://doi.org/10.3892/ijmm.2016.2488
Pages: 958-966
Copyright: © Iavello et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Evidence indicates that Alix, an accessory protein of the endosomal sorting complex required for transport (ESCRT), is involved in the biogenesis of extracellular vesicles (EVs). EVs contain selected patterns of microRNAs (miRNAs or miRs); however, little is known about the mechanisms of miRNA enrichment in EVs. The aim of the present study was to evaluate whether Alix is involved in the packaging of miRNAs within EVs released by human liver stem‑like cells (HLSCs). EVs released from HLSCs were enriched with miRNAs and expressed Alix and several RNA-binding proteins, including Argonaute 2 (Ago2), a member of the Argonaute family known to be involved in the transport and the processing of miRNAs. Co-immunoprecipitation experiments revealed an association between Alix and Ago2. The results from RT-qPCR indicated that in the Alix/Ago2 immunoprecipitates, miRNAs were detectable. EVs were instrumental in transferring selected miRNAs from HLSCs to human endothelial cells absent in the latter cells. Alix knockdown did not influence the number of EVs released by HLSCs, but it significantly decreased miRNA expression levels in the EVs and consequently their transfer to the endothelium. Our findings indicate that Alix binds to Ago2 and miRNAs, suggesting that it plays a key role in miRNA enrichment during EV biogenesis. These results may represent a novel function of Alix, demonstrating its involvement in the EV-mediated transfer of miRNAs.

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