Ginkgolide B promotes cell growth in endothelial progenitor cells through miR-126 and the Akt signaling pathway

  • Authors:
    • Jing Chang
    • Xin Xue
    • Chunli Song
    • Bin Liu
    • Lihua Gao
  • View Affiliations

  • Published online on: August 16, 2017     https://doi.org/10.3892/mmr.2017.7254
  • Pages: 5627-5632
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Abstract

Ginkgolide B is one of the components extracted from Folium Ginkgo. It is a natural antagonist of platelet‑activating factor receptor and has multiple pharmacological applications. In the present study, the effect of ginkgolide B on the proliferation of endothelial progenitor cells (EPCs) was examined, as well as its potential underlying mechanism. EPCs were cultured in various concentrations of ginkgolide B (0, 1.25, 5, 20, 80 or 160 µg/ml) for 24 or 48 h and then numbers of viable cells, apoptosis rate and caspace‑3 activity were measured. The results demonstrated that ginkgolide B treatment effectively promoted EPC growth, and suppressed cell apoptosis and caspase‑3 activity compared with control cells. In addition, ginkgolide B treatment significantly induced the expression of miR‑126, vascular endothelial growth factor and endothelial nitric oxide synthase, while it increased phosphorylation of AKT serine/threonine kinase 1 (Akt) and of p38 mitogen‑activated protein kinase in EPCs. The present study therefore demonstrated that ginkgolide B promoted cell growth in EPCs through overexpression of miR‑126 and activation of the Akt signaling pathway.

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October-2017
Volume 16 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Chang J, Xue X, Song C, Liu B and Gao L: Ginkgolide B promotes cell growth in endothelial progenitor cells through miR-126 and the Akt signaling pathway. Mol Med Rep 16: 5627-5632, 2017
APA
Chang, J., Xue, X., Song, C., Liu, B., & Gao, L. (2017). Ginkgolide B promotes cell growth in endothelial progenitor cells through miR-126 and the Akt signaling pathway. Molecular Medicine Reports, 16, 5627-5632. https://doi.org/10.3892/mmr.2017.7254
MLA
Chang, J., Xue, X., Song, C., Liu, B., Gao, L."Ginkgolide B promotes cell growth in endothelial progenitor cells through miR-126 and the Akt signaling pathway". Molecular Medicine Reports 16.4 (2017): 5627-5632.
Chicago
Chang, J., Xue, X., Song, C., Liu, B., Gao, L."Ginkgolide B promotes cell growth in endothelial progenitor cells through miR-126 and the Akt signaling pathway". Molecular Medicine Reports 16, no. 4 (2017): 5627-5632. https://doi.org/10.3892/mmr.2017.7254