A polysaccharide component from Strongylocentrotus nudus eggs inhibited hepatocellular carcinoma in mice by activating T lymphocytes

  • Authors:
    • Min Zhang
    • Yang Liu
    • Jingwen Li
    • Mengyun Ke
    • Jie Yu
    • Jie Dou
    • Hui Wang
    • Changlin Zhou
  • View Affiliations

  • Published online on: January 19, 2017     https://doi.org/10.3892/ol.2017.5624
  • Pages: 1847-1855
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Abstract

A component purified from Strongylocentrotus nudus eggs on a diethylaminoethyl cellulose‑52 chromatography column and eluted using a NaCl solution gradient (SEP‑S), is a homogeneous polysaccharide of α‑D‑glucan with a reduced molecular weight of 9.33x105 Da, compared with that of S. nudus egg polysaccharide (SEP). In an in vivo antitumor assay of histocompatibility‑22 hepatocellular carcinoma in tumor‑bearing mice, the inhibitory rates at SEP‑S doses of 5, 10 and 20 mg/kg/day were 38.8, 50.7 and 70.3%, respectively. In addition, the spleen and thymus indices and the percentages of cluster of differentiation (CD) 4+ and CD8+ T cells were significantly increased, and the activity of cytotoxic T lymphocytes was notably enhanced, suggesting that the anti‑hepatocellular carcinoma activity is mediated by boosting the immune system. In vitro experiments also demonstrated that splenocyte proliferation induced by SEP‑S was inhibited by the toll‑like receptor (TLR) 2 and TLR4 monoclonal antibodies. These data indicate that SEP‑S is a polysaccharide component possessing high anti‑hepatocellular carcinoma activity and may be a potential immunotherapy candidate for the treatment of liver cancer.
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March-2017
Volume 13 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Zhang M, Liu Y, Li J, Ke M, Yu J, Dou J, Wang H and Zhou C: A polysaccharide component from Strongylocentrotus nudus eggs inhibited hepatocellular carcinoma in mice by activating T lymphocytes. Oncol Lett 13: 1847-1855, 2017
APA
Zhang, M., Liu, Y., Li, J., Ke, M., Yu, J., Dou, J. ... Zhou, C. (2017). A polysaccharide component from Strongylocentrotus nudus eggs inhibited hepatocellular carcinoma in mice by activating T lymphocytes. Oncology Letters, 13, 1847-1855. https://doi.org/10.3892/ol.2017.5624
MLA
Zhang, M., Liu, Y., Li, J., Ke, M., Yu, J., Dou, J., Wang, H., Zhou, C."A polysaccharide component from Strongylocentrotus nudus eggs inhibited hepatocellular carcinoma in mice by activating T lymphocytes". Oncology Letters 13.3 (2017): 1847-1855.
Chicago
Zhang, M., Liu, Y., Li, J., Ke, M., Yu, J., Dou, J., Wang, H., Zhou, C."A polysaccharide component from Strongylocentrotus nudus eggs inhibited hepatocellular carcinoma in mice by activating T lymphocytes". Oncology Letters 13, no. 3 (2017): 1847-1855. https://doi.org/10.3892/ol.2017.5624