Hydrogen sulfide upregulates heme oxygenase‑1 expression in rats with volume overload‑induced heart failure

  • Authors:
    • Chao‑Ying Zhang
    • Xiao‑Hui Li
    • Ting Zhang
    • Jin Fu
    • Xiao‑Dai Cui
  • View Affiliations

  • Published online on: March 26, 2013     https://doi.org/10.3892/br.2013.87
  • Pages: 454-458
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The present study investigated the role of hydrogen sulfide (H2S), a novel gaseous transmitter, in chronic heart failure (CHF) induced by left‑to‑right shunt, leading to volume overload. Thirty male Sprague‑Dawley rats were randomly divided into four groups: the shunt group, the sham group, the shunt + sodium hydrosulfide (NaHS) group and the sham + NaHS group. CHF was induced in the rats by abdominal aorta‑inferior vena cava shunt operation. Rats in the shunt + NaHS and sham + NaHS groups were injected intraperitoneally with NaHS (H2S donor). Haemodynamic parameters were measured 8 weeks after surgery. In addition, left ventricular heme oxygenase (HO)‑1 mRNA expression was measured by real‑time PCR. Protein expression of HO‑1 was evaluated by western blot analysis. Eight weeks after surgery, compared to the sham group, the left ventricular systolic pressure (LVSP) and left ventricular peak rate of contraction and relaxation (LV±dp/dtmax) were significantly reduced; the left ventricular end‑diastolic pressure (LVEDP) was significantly increased in the shunt group (all P<0.05). However, NaHS increased LVSP and LV±dp/dtmax (all P<0.05) and decreased LVEDP (P<0.05). Protein expression of HO‑1 was significantly decreased in the shunt group compared to that in the sham group (P<0.05). NaHS increased protein expression of HO‑1 compared to that in the shunt group (P<0.05). HO‑1 mRNA expression was significantly increased in the shunt + NaHS group compared to that in the shunt group (P<0.01). The present study demonstrated that H2S may play a protective role in volume overload‑induced CHF by upregulating protein and mRNA expression of HO‑1.
View Figures
View References

Related Articles

Journal Cover

May-June 2013
Volume 1 Issue 3

Print ISSN: 2049-9434
Online ISSN:2049-9442

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Zhang CY, Li XH, Zhang T, Fu J and Cui XD: Hydrogen sulfide upregulates heme oxygenase‑1 expression in rats with volume overload‑induced heart failure. Biomed Rep 1: 454-458, 2013
APA
Zhang, C., Li, X., Zhang, T., Fu, J., & Cui, X. (2013). Hydrogen sulfide upregulates heme oxygenase‑1 expression in rats with volume overload‑induced heart failure. Biomedical Reports, 1, 454-458. https://doi.org/10.3892/br.2013.87
MLA
Zhang, C., Li, X., Zhang, T., Fu, J., Cui, X."Hydrogen sulfide upregulates heme oxygenase‑1 expression in rats with volume overload‑induced heart failure". Biomedical Reports 1.3 (2013): 454-458.
Chicago
Zhang, C., Li, X., Zhang, T., Fu, J., Cui, X."Hydrogen sulfide upregulates heme oxygenase‑1 expression in rats with volume overload‑induced heart failure". Biomedical Reports 1, no. 3 (2013): 454-458. https://doi.org/10.3892/br.2013.87