Predominance of precore mutations and clinical significance of basal core promoter mutations in chronic hepatitis B virus infection in Indonesia

  • Authors:
    • Juniastuti
    • Takako Utsumi
    • Eduardus Bimo Aksono
    • Yoshihiko Yano
    • Soetjipto
    • Yoshitake Hayashi
    • Hak Hotta
    • Fedik Abdul Rantam
    • Hernomo Ontoseno Kusumobroto
    • Maria Inge Lusida
  • View Affiliations

  • Published online on: May 15, 2013     https://doi.org/10.3892/br.2013.106
  • Pages: 522-528
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Abstract

Chronic hepatitis B virus (HBV) infection is a major health problem worldwide, with a particularly high prevalence in the Asian‑Pacific region. During chronic hepatitis B virus (HBV) infection, mutations commonly occur in the basal core promoter (BCP) and precore (PC) regions of HBV, affecting HBeAg expression, particularly following HBeAg seroconversion. Mutations in the B‑ and T‑cell epitopes of the HBV core have also been observed during disease progression. The clinical significance of HBV genome variability has been demonstrated, however the results are a subject of controversy. Considering the characteristics of the virus associated with geographical location, the profiles of BCP, PC and core mutations and their clinical implications in patients with chronic HBV infection in Surabaya, Indonesia, were investigated. The BCP, PC and core mutations and HBV genotypes were detected by direct sequencing. The HBeAg̸anti‑HBe status and HBV DNA levels were also assessed. This study enrolled 10 patients with chronic HBV infection (UC) from Dr Soetomo General Hospital and Indonesian Red Cross, Surabaya, East Java, Indonesia, 10 patients with chronic hepatitis B and liver cirrhosis (LC) and 4 patients with chronic hepatitis B and hepatocellular carcinoma (HCC) from Dr Soetomo General Hospital. The PC mutation A1896 was predominant in all the groups (60‑100%), together with the PC variant T1858, which was associated with HBV genotype B. The number of detected core mutations (Thr/Ser130) was higher in HCC patients (50%). However, the BCP mutations T1762/A1764 were predominant in LC patients (50‑60%). The LC and HCC patients carried HBV isolates with additional mutations, at least at BCP or PC, mainly following HBeAg seroconversion. In the majority of anti‑HBe‑positive samples, the BCP T1762̸A1764 mutations were associated with a high viral load, regardless of the PC 1896 status. In conclusion, the PC mutations were found to be predominant in all the groups. However, the BCP mutations were mainly detected in the LC group and may be considered as a critical indicator of a poor clinical outcome.

Introduction

Chronic hepatitis B virus (HBV) infection is a major clinical problem worldwide. It is particularly important in the Asian-Pacific region where the prevalence of HBV infection is high (1), including Indonesia, which belongs to the moderate-to-high hepatitis B endemic regions (2,3). Chronically infected patients exhibit a wide spectrum of clinical presentations, ranging from an asymptomatic carrier state to chronic active hepatitis B with progression to liver failure, liver cirrhosis (LC) or hepatocellular carcinoma (HCC) (4,5). It has been hypothesized that the genetic variability of the virus, which affects its expression of viral antigens, may also affect the outcome of the infection (6). However, the results of a previous molecular study are a subject of controversy and these issues require further elucidation (7).

It has been suggested that HBeAg may serve as a decoy to buffer the anti-core protein immune response or to induce immune tolerance in perinatally infected individuals. However, the anti-HBe immune response results in an efficient reduction of viral load and thereby provides a strong pressure toward viral variants with reduced or no HBeAg expression (8,9). Two major types of HBV variants that frequently occur and affect the expression of HBeAg are the precore (PC) and the basal core promoter (BCP) mutants (9). The most frequently detected PC mutation is a nucleotide (nt) transition at the codon 28 (A1896) which converts into a TAG stop codon and eliminates HBeAg expression (10,11). Previously reported evidence suggested that the pattern of PC mutation is restricted by the secondary structure requirements of the ε encapsidation signals which are essential for viral replication. In order to stabilize this ε structure, the nt at position 1,896 is paired with the nt at position 1,858, which is naturally specific for certain HBV genotypes (12,13). The most common BCP mutations, A1762T and G1764A, result in a decrease of HBeAg expression, mediated by reduced transcription of PC RNA, but enhance viral replication (8,14,15). Occurrence of these mutations and any additional BCP mutations may confer increased replication efficiency to the virus (9,16). These changes were previously considered to be associated with the HBeAg-negative phenotype. However, more recent studies demonstrated that they may also be found in certain HBeAg-positive patients, particularly those with chronic hepatitis. These BCP mutations may be detected with or without PC mutations (17-19). Core mutations, particularly amino acid (aa) at position 130 exposed on the surface of mature HBeAg, were observed in the course of disease progression and accumulate in the B- and T-cell epitopes (20,21). These findings emphasize the possibility that the mutations in the T- or B-cell epitopes exert a significant effect on T-cell function or subsequent cytokine release and on the association between the host immunological reaction and viral persistence.

A previous study suggested that HBeAg mutations are associated with chronic hepatitis, LC and HCC (13). However, conflicting evidence suggested that the HBeAg mutations are present in HBV carriers and in those individuals with mild forms of HBV infection or without liver disease (7). In addition, several core mutations have not been proven to result in loss of immune recognition (22).

The aim of this study was to analyze the profiles of BCP, PC and core mutations associated with the HBeAg/anti-HBe status and HBV DNA load in patients with chronic HBV infection at various phases in Surabaya, Indonesia. The characteristic of the HBV isolates associated with geographical location may play an important role in these results.

Materials and methods

Patients and controls

Three groups of patients were investigated: i) the UC group included patients who had been HBsAg-positive for >6 months, although without any clinical significance, from Dr Soetomo General Hospital, Surabaya, Indonesia and HBsAg-positive blood donors with asymptomatic liver disease from the Blood Transfusion Unit, Indonesian Red Cross, Surabaya; ii) the LC group included HBV-infected patients with LC; and iii) the HCC group included HBV-infected patients with HCC from Dr Soetomo General Hospital. Determination of the groups from Dr Soetomo General Hospital was performed by clinical and biochemical data; ultrasonographic data were added to confirm the LC and HCC groups. Blood donors were included in the UC group if the laboratory examinations verified immunoglobulin M (IgM) anti-hepatitis B core (HBc)-negativity and normal alanine transaminase levels. None of the patients had a history of antiviral drug use. Ethical clearance for the present study was obtained from the Ethics Committee of the Dr Soetomo General Hospital, Surabaya. All the participants signed a consent form.

Serological markers

HBsAg, HBeAg, anti-HBe antibody and other serological markers (anti-HCV and anti-HIV antibodies) were detected by a microparticle enzyme immunoassay (Abbott, Wiesbaden, Germany). Blood donors were screened for IgM anti-HBc to exclude acute HBV infection. The anti-HCV and anti-HIV antibodies were also tested to confirm the absence of exclusion criteria.

Viral DNA extraction, PCR amplification and sequencing

HBV DNA was extracted from each serum sample using the High Pure Viral Nucleic Acid kit (Roche Molecular Systems, Inc., Alameda, CA, USA) following the manufacturer’s insructions. The extracted DNA was used as a template for the amplification of the respective gene regions. Polymerase chain reactions (PCRs) were performed with the High Fidelity PCR enzyme mix (Fermentas, Vilnius, Lithuania). The reactions included 2.5 μl High Fidelity PCR buffer (with MgCl2), 2.5 μl dNTP with a concentration of 2 mM, 0.25 μl High Fidelity PCR enzyme mix, 10 μl DNA and 0.5 μl of each primer with a concentration of 100 pmol/μl, in a total reaction volume of 25 μl. The thermocycling conditions included a 5-min denaturation step at 94°C, followed by 40 cycles of 1 min at 94°C, 1 min at 55°C and 2 min at 72°C (23).

The BCP, PC and partial core genes were amplified in the first round using HBc1 (5′-TTA CAT AAG AGG ACT CTT GG-3′, nt 1,650-1,669) and HB9R (5′-GGA TAG AAC TAG CAG GCA T-3′, nt 2,654-2,635) (24). If the first-round PCR was negative, the second-round PCR was performed using primers HBc1 and HBc2 (5′-TAA AGC CCA GTA AAG TTT CC-3′, nt 2,494-2,475) (25).

To elucidate the association between HBV genotype and PC mutations, part of the S gene was amplified in the first round using primers P7 (5′-GTG GTG GAC TTC TCT CAA TTT TC-3′, nt 256-278) and P8 [5′-CGG TAW(A/T) AAA GGG ACT CAM(A/C) GAT-3′, nt 796-776]. If the first-round PCR was negative, the second-round PCR was performed using primers HBs1 (5′-CAA GGT ATG TTG CCC GTT TG-3′, nt 455-474) and HBs2 (5′-AAA GCC CTG CGA ACC ACT GA-3′, nt 713-694) (23). The nt sequences of the amplified fragments were determined using the BigDye Terminator v1.1 Cycle Sequencing kit with an ABI Prism 310 (Applied Biosystems, Foster City, CA, USA).

Sequences analyses for the detection of BCP, PC and core mutations

All sequence data analyses were performed with Genetyx-Windows version 9 (Software Development Co., Ltd., Tokyo, Japan). After aligning the sequences obtained from this study and from International DNA databases (DDBJ/EMBL/GenBank), the variability of the BCP, PC and core was analyzed. The HBV genotypes were determined based on the homologous percentage of >96% in the S and/or core gene sequences compared to HBV isolates from International DNA databases (DDBJ/EMBL/GenBank) (26,27).

Quantification of HBV DNA

The HBV DNA level was determined with the COBAS Amplicor HBV monitor (Roche Molecular Systems, Inc., Branchburg, NJ, USA).

INNO-LiPA PC assay

The INNO-LiPA PreCore kit (Innogenetics, Ghent, Belgium) was used to assess serum samples (with A1896 PC mutation but HBeAg-positive) which could not be assessed by direct sequencing. The kit is able to detect a wild-type/mutant mixed population of circulating virus.

Results

Profiles of BCP, PC and core mutations and HBV genotype

A total of 29 HBsAg-positive serum samples were obtained from patients and control subjects. In 24 of the 29 samples the sequences of BCP, PC and core regions were detected and confirmed. The samples were collected from 10 patients with UC, 10 with LC and 4 patients with HCC. All 24 subjects were aged 22-68 years (mean age, 43.8 years) and included 18 men and 6 women. Their descent was from Java (88%), Flores (4%), Sulawesi (4%) and Aceh-Batak (4%) and they resided in Surabaya.

In the 24 serum samples, the most frequently detected mutation in the PC region was A1896 (67%) and it was predominant in all the groups (60-100% of each group) (Table I). The PC A1896 mutation was encountered in all isolates identified as genotype B (data not shown). Based on part of the S and/or core genes, the 24 isolates were classified into HBV geno-type B. The association between genotype B and the PC variant T1858 was 100%. The other PC mutation, A1899, was detected in 21% isolates (5/24) and in some of them it was observed in combination with A1896 (3/5, 60%). In the BCP region, T1762 and A1764 mutations were detected in 38% and 33% isolates, respectively, and these mutations were predominant in the LC group (50-60%). Mutations in the core region aa 130 were detected in six (25%) isolates (10-50% of each group), mostly in the HCC group. The obtained mutation types in core aa 130 included Thr130 (five isolates) and Ser130 (one isolate). The other core mutation, Leu97, was encountered in 38% isolates (30-50% of each group). The core mutations, Thr130 combined with Leu97, were observed in three isolates (3/5, 60%).

Table I.

Number of BCP, PC and core mutations in various clinical stages of chronic HBV infection.

Table I.

Number of BCP, PC and core mutations in various clinical stages of chronic HBV infection.

Clinical stageNo.No. of HBV mutations/variants
BCP
PC
Core
T1762 (%)A1764 (%)T1858 (%)A1896 (%)A1899 (%)Leu97 (%)Thr/Ser130 (%)
UC102/10 (20)2/10 (20)10/10 (100)6/10 (60)3/10b(30)3/10 (30)1/10d(10)
LC106/10 (60)5/10 (50)10/10 (100)6/10 (60)2/10a(20)4/10 (40)3/10e(30)
HCC41/4 (25)1/4 (25)4/4 (100)4/4 (100)0/4 (0)2/4 (50)2/4c(50)
Total249/24 (38)8/24 (33)24/24 (100)16/24 (67)5/24 (21)9/24 (38)6/24 (25)

{ label (or @symbol) needed for fn[@id='tfn1-br-01-04-0522'] } Mutations of BCP and PC are presented at nucleotide positions, but core mutations are presented at amino acid (aa) positions.

a One sample exhibited combined PC A1899 and PC A1896 mutations;

b two samples exhibited combined PC A1899 and PC A1896 mutations;

c all the core mutations at aa 130 were Thr130, however, 1 sample in the HCC group exhibited Ser130 mutation;

d one sample exhibited combined Thr130 and Leu97 core mutations;

e two samples exhibited combined Thr130 and Leu97 core mutations. BCP, basal core promoter; PC, precore; HBV, hepatitis B virus; UC, chronic HBV infection; LC, liver cirrhosis; HCC, hepatocellular carcinoma; Leu, leucine; Thr, threonine; Ser, serine.

To simplify data analysis, this study focused on BCP T1762/A1764, PC A1896 and core Thr/Ser130 as the well-known hotspot mutations. The mutations of PC A1899 and core Leu97 were also included, since they could be found in combination and share certain effects with the mutations mentioned above (PC A1896 with A1899 and core Leu97 with Thr/Ser130) (Table II). There were several basic pattern possibilities, i.e., no mutations (BCP− PC− C−), BCP mutation only (BCP+ PC− C−), BCP and PC mutations (BCP+ PC+ C−), BCP and C mutations (BCP+ PC− C+), PC mutation only (BCP− PC+ C−), PC and core mutations (BCP− PC+ C+), core mutation only (BCP− PC− C+) and triple mutations (BCP+ PC+ C+). The pattern of core mutation only (BCP− PC− C+) was not detected in this study. The LC and HCC groups carried HBV isolates with additional mutations, at least BCP or PC mutations. The LC group mainly carried HBV with BCP mutations (60%), whereas the HCC group commonly exhibited PC mutations (100%). The triple mutation pattern (BCP+ PC+ C+) was only detected in the LC and HCC groups; however, the BCP− PC− C− pattern was absent in the UC group.

Table II.

Patterns of BCP, PC and core mutations in various clinical stages of chronic HBV infection.

Table II.

Patterns of BCP, PC and core mutations in various clinical stages of chronic HBV infection.

Clinical stageNo.Patterns of BCP, PC and core mutations
BCP+ PC− C−BCP+ PC+ C−BCP+ PC− C+BCP+ PC+ C+BCP− PC+ C−BCP− PC+ C+BCP− PC− C−
UC10-2 (20%)--2 (20%)3 (30%)3 (30%)
LC102 (20%)1 (10%)1 (10%)2 (20%)2 (20%)2 (20%)-
HCC4---1 (25%)1 (25%)2 (50%)-
Total242 (8%)3 (13%)1 (4%)3 (13%)5 (21%)7 (29%)3 (13%)

[i] BCP mutations, mutations of T1762/A1764 in the BCP region; PC mutations, mutations of A1896 and/or A1899 in the PC region; C mutations, mutations of Leu97 and/or Thr/Ser130 in the core region; +, mutant type; -, wild-type. BCP, basal core promoter; PC, precore; C, core; HBV, hepatitis B virus; UC, chronic HBV infection; LC, liver cirrhosis; HCC, hepatocellular carcinoma; Leu, leucine; Thr, threonine; Ser, serine.

Status of HBeAg/anti-HBe and HBV DNA level associated with BCP, PC and core mutations

Of the 24 serum samples, HBeAg-negative/anti-HBe-positive was the predominant type (71%), followed by HBeAg-positive/anti-HBe-negative (25%) and HBeAg-negative/anti-HBe-negative (4%). Anti-HBe-positive was predominant in the HCC group (100%), followed by the LC (70%) and UC (60%) groups (Table III).

Table III.

Status of HBeAg/anti-HBe in various clinical stages of chronic HBV infection.

Table III.

Status of HBeAg/anti-HBe in various clinical stages of chronic HBV infection.

Clinical stageNo.Status of HBeAg/anti-HBe (%)
HBeAg(+)/anti-HBe(−) HBeAg(−)/anti-HBe(+) HBeAg(−)/anti-HBe(−)
UC103 (30)6 (60)1 (10)
LC103 (30)7 (70)0
HCC404 (100)0
Total246/24 (25)17/24 (71)1/24 (4)

[i] HBV, hepatitis B virus; UC, chronic HBV infection; LC, liver cirrhosis; HCC, hepatocellular carcinoma; (+), positive; (−), negative.

In the six HBeAg-positive serum samples (prior to sero-conversion), the number of wild-types of BCP (A1762/G1764), PC (G1896 and G1899) and core (Ile97 and Pro130) was higher (range, 66.7-100%) compared with the number of mutant types of BCP (T1762/A1764), PC (A1896 and A1899) and core (Thr/Ser130) (Table IV). As determined by the INNO-LiPA assay, the two samples from patient 7RS (UC group) and patient 17RS (LC group) that were HBeAg-positive in the presence of the PC A1896 mutation, had mixed strains of mutant and wild-types of the PC region.

Table IV.

BCP, PC and core mutations of HBV prior to and following HBeAg seroconversion.

Table IV.

BCP, PC and core mutations of HBV prior to and following HBeAg seroconversion.

Time pointNo.BCP
PC
Core
nt 1762/1764
nt 1896
nt 1899
aa 97
aa 130
WT (%)MT (%)WT (%)MT (%)WT (%)MT (%)WT (%)MT (%)WT (%)MT (%)
Prior to SC64 (66.7)2 (33.3)4 (66.7)2 (33.3)6 (100)04 (66.7)2 (33.3)4 (66.7)2 (33.3)
Following SC1710 (58.9)7 (41.2)3 (23.5)14 (76.5)12 (70.6)5 (29.4)10 (58.9)7 (41.2)13 (76.5)4 (23.5)

[i] BCP, basal core promoter; PC, precore; HBV, hepatitis B virus; nt, nucleotide; aa, amino acid; WT, wild-type; MT, mutant type; SC, seroconversion.

Following HBeAg seroconversion, the number of the mutant types of BCP nt 1,762/1,764 was lower compared to the wild-types, although the number was increased compared to prior to seroconversion. However, the number of core mutations Thr/Ser130 was lower following compared to prior to seroconversion. Of note, the number of the mutant type of PC A1896 mutations was significantly higher (82.4%) in anti-HBe-positive serum samples compared to that of wild-type mutations (17.7%) (Table IV).

Following HBeAg seroconversion, the mutant types of BCP (T1762/A1764) were frequently associated with a high level of HBV DNA (57.1%). However, the number of serum samples with the mutant type of PC A1896 exhibiting low HBV DNA levels and that of samples with high HBV DNA levels were identical. Of the three samples with the mutant type of combined PC A1899 and PC A1896, two were associated with a low level of HBV DNA (Table V).

Table V.

Mutations of BCP, PC and core according to the HBV DNA level in anti-HBe(+) samples.

Table V.

Mutations of BCP, PC and core according to the HBV DNA level in anti-HBe(+) samples.

Mutations of HBeAgHBV DNA level
Low [<5 log copies/ml (%)]High [≥5 log copies/ml (%)]
BCP T1762/A17643/7 (42.9)4/7 (57.1)
PC A18967/14 (50)7/14 (50)
PC A1899 + A18962/3 (66.7)1/3 (33.3)

[i] BCP, basal core promoter; PC, precore; HBV, hepatitis B virus; (+), positive.

Discussion

HBV mutations in the PC, BCP and core regions have been reported to exert various effects on the clinical course of patients with HBV-related liver diseases (28). In this study, these mutations were assessed in various phases of chronic hepatitis B infection in Surabaya, Indonesia.

In the PC region, the predominant mutation is a G-to-A change at nt 1,896 (A1896), which creates a premature stop codon and eliminates the synthesis of HBeAg (29). A previous study demonstrated that HBeAg may be a target antigen on HBV-infected hepatocytes (30) and failure to produce a target antigen may be a means of evading immune clearance. It was reported that there is a significant association between PC mutations and remission of liver disease (31). However, other studies reported a high prevalence of PC mutations in patients with severe liver disease (32-34). The results of this study have demonstrated that in the PC region, the A1896 mutant was predominant in patients with HCC (100%) and those with LC (60%), although it was also found in UC patients (60%) (Table I). The variability in the prevalence of the A1896 mutant in different geographical regions is associated with the predominant HBV genotype, since this mutant is restricted to the HBV genotypes with T at nt 1,858 and is not encountered in those with C at nt 1,858 (13,35). The A1896 and T1858 tighten the stem structure by forming a T-A pair (12), although T1858 may also form a wobble pairing with G1896 (11). The PC A1896 is found only in patients infected with HBV genotypes B, D, E and in a minority of those infected with the C and F strains that bear a variant T at nt 1,858 (36,37). In accordance with previous studies (23,38), this study has demonstrated that HBV geno-type B was predominant, detected in all 24 serum samples collected mainly from individuals of Java origin (88%), as well as of Sulawesi, Aceh-Batak and Flores origin (4% each) who resided in Surabaya. The HBV genotypes also appear to be associated with ethnic origin. However, the association between the A1896 in the PC region and other genotypes could not be elucidated in this study. As regards clinical outcomes, our results were in accordance with those previously reported (39), stating that A1896 alone may have no direct pathogenic role, particularly in HBV isolates with genotypes harbouring T1858. The other PC mutation, A1899, was detected in three serum samples combined with A1896. These combined mutations may enhance the stability of the stem loop which is essential for viral replication (40). However, in this study, in two out of the three samples, low HBV DNA levels were detected (Table V). Additional studies are required to confirm the significance of these mutations on viral replication.

In the BCP region, the most common mutations involve a two-nt substitution: A-T at nt 1,762 and G-A at nt 1,764 (T1762 and A1764) (17). Previous transfection studies demonstrated that the T1762 and A1764 mutations decrease the level of pre-C mRNA by 50–70% and lead to reduced HBeAg synthesis (14,41). The BCP mutants may enhance HBV virulence by increasing host immune response to HBV-infected hepatocytes, increasing viral replication or altering the coding region for the X (14,15,42,43). The BCP T1762/A1764 were found in patients of the HCC (25%) and UC (20%) groups, although the majority was encountered in the LC group (50–60%) (Table I). This finding was in agreement with a previous study which reported that BCP mutations were found mainly in cirrhotic tissues with a lower incidence in HCC tissues (44). Of note, the majority of isolates (55.5%, 5/9) with BCP T1762/A1764 were not accompanied by PC A1896. It was suggested that BCP mutations frequently emerge during the late HBeAg phase of infection, whereas PC mutations usually emerge later, at the height of the anti-HBe immune response (9). In this study, the two HBeAg-positive serum samples with BCP mutations exhibited no concomitant PC mutation; however, four out of the seven anti-HBe-positive samples with BCP mutations also exhibited PC mutations.

Mutant HBV may also exhibit enhanced virulence with alteration of epitopes which is critical for the host immune response. Mutations in B- and T-cell epitopes are associated with viral persistence, affecting the host immune response (4547). The inflammatory activity produced by viral adaptive mechanisms may persist in up to 15% of cases, leading to the development of cirrhosis (48). A previous study by Hosono et al (20) suggested that core mutations in HBV accumulated more errors in tumors compared to non-tumors. The aa 120-140 in the core region exposed on the surface of mature HBeAg and HBcAg are related to the recognition of helper T cells (4951) and the immunodominant B-cell recognition sites within the HBcAg have also been found around residues 126-135 (45,46). One of the frequent core mutations is at aa 130, predominated by Thr130 [67 out of 96 (70%)]. It was noted that Thr130 was frequently associated with the occurrence of Leu97 [50 out of 67 (75%)], although it may occur per se [17 out of 67 (25%)] (52). The Ser130 mutation was also reported to affect cellular and humoral immunity, since the codon is part of a domain which is recognized by B and T cells (53). The Leu97 of HBcAg was reported to enable the virus to secrete an excessive amount of immature genome with nascent incomplete single-strand DNA in an envelope-dependent manner, leading to attenuation of the total yield of mutant virus production (52,54,55). However, this excessive secretion of incomplete virions may be offset by an additional mutation at codon 130 (Pro to Thr/Ser) (52,53). In this study, the Thr/Ser130 mainly occurred in the HCC group (2/4, 50%), followed by the LC (30%) and UC (10%) groups. The Thr/Ser130 was mostly detected in combination with Leu97 (Table I).

Certain combined mutations in the BCP, PC and core regions are of higher clinical significance regarding the severity of liver disease. In this study, patients with LC and HCC carried HBV with mutations of at least BCP or PC. Some patients with UC also carried the HBV mutation; however, more mutations were likely to be associated with LC and HCC (Table II). The combination of mutations rather than a single mutation was associated with the development of progressive liver disease (56).

The most frequently encountered variant of chronic HBV infections is HBeAg-negative chronic hepatitis B. It may follow seroconversion from HBeAg to anti-HBe antibodies during the immune reactive phase or develop after years or decades of inactive carrier state. The patients are HBeAg-negative and harbour a predominance of HBV virions with nt substitutions in the PC and/or the BCP regions that lead to absent or low expression levels of HBeAg (5,57). HBeAg-negative mutants frequently become the predominant virus population in chronic HBsAg carriers, possibly indicating a selection advantage against the wild-type (10,17). This study has demonstrated that HBeAg-negative/anti-HBe -positive was the predominant type (71%) in all the groups, particularly in the HCC group (100%) (Table III). Following seroconversion, PC A1896 was predominant and the number of the BCP T1762/A1764 mutations was increased (Table IV). In the majority of anti-HBe-positive serum samples, the BCP T1762/A1764 was mostly correlated with a high viral load (≥105 copies/ml), but PC A1896 was not correlated with viral load level (Table V). This finding was in agreement with previously reported results (58), suggesting that patients with the BCP T1762/A1764 mutant exhibited significantly higher serum HBV DNA levels compared to those with the BCP A1762/G1764 wild-type strain, regardless of the PC 1896 status. The BCP mutations were found in the dominant viral species at the late HBeAg-positive and early anti-HBe phases of HBV infection. Thus, chronic hepatitis B patients infected with the T1762 and A1764 mutants may have a longer duration of active replication (58). The number of the core mutation Thr130 was lower following seroconversion compared with prior to seroconversion (Table IV). The duration of serocon-version from HBeAg to anti-HBe may be attributed to the extent of immunological attacks against the HBV core region.

Of note, two serum samples collected from one patient of the UC (7RS) and one of the LC group (17RS) were HBeAg-positive in the presence of the PC A1896 mutation. This observation may be due to the presence of a mixed infection by the mutant- and wild-type viruses. The presence of the wild-type virus is required by certain mutants for the infection of hepatocytes. It is likely that HBV exists as a quasi species of wild-type and mutant clones, even in the HBeAg-positive phase (7). To determine any mixed infection (59), the two samples were examined by the INNO-LiPA assay and it was confirmed that they had mixed strains of mutant and wild-type PC. A heterogeneous virus population circulating in patients with chronic HBV infection may thus determine the outcome of infection.

In conclusion, the PC mutation A1896 was predominant in all the groups and the BCP mutations T1762/A1764 were only predominant in patients with chronic hepatitis B and LC. The BCP mutations may be considered as a more efficient indicator of a poor outcome compared to the PC mutations. Additional studies are required, including a larger population, to determine which type of specific mutations or combined mutations is associated with liver disease severity and may thus be involved in the pathogenetic process.

Acknowledgements

The authors are grateful to Slamet Riyadi, Nur Achmad Tjipto and Nasronudin for their cooperation and to Mochamad Amin and Koen Poedijati for their technical assistance. We are also grateful to the patients at Dr Soetomo General Hospital, Surabaya and the blood donors at the Blood Transfusion Unit-Indonesia Red Cross, Surabaya, who provided the blood samples for this study. This study was supported by a grant from the Directorate General of Higher Education, Department of National Education, Indonesia and by the Japan Initiative for Global Research Network on Infectious Diseases, the Ministry of Education, Culture, Sports, Science and Technology, Japan.

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Spandidos Publications style
Juniastuti , Utsumi T, Aksono EB, Yano Y, Soetjipto , Hayashi Y, Hotta H, Rantam FA, Kusumobroto HO, Lusida MI, Lusida MI, et al: Predominance of precore mutations and clinical significance of basal core promoter mutations in chronic hepatitis B virus infection in Indonesia. Biomed Rep 1: 522-528, 2013
APA
Juniastuti, ., Utsumi, T., Aksono, E.B., Yano, Y., Soetjipto, ., Hayashi, Y. ... Lusida, M.I. (2013). Predominance of precore mutations and clinical significance of basal core promoter mutations in chronic hepatitis B virus infection in Indonesia. Biomedical Reports, 1, 522-528. https://doi.org/10.3892/br.2013.106
MLA
Juniastuti, ., Utsumi, T., Aksono, E. B., Yano, Y., Soetjipto, ., Hayashi, Y., Hotta, H., Rantam, F. A., Kusumobroto, H. O., Lusida, M. I."Predominance of precore mutations and clinical significance of basal core promoter mutations in chronic hepatitis B virus infection in Indonesia". Biomedical Reports 1.4 (2013): 522-528.
Chicago
Juniastuti, ., Utsumi, T., Aksono, E. B., Yano, Y., Soetjipto, ., Hayashi, Y., Hotta, H., Rantam, F. A., Kusumobroto, H. O., Lusida, M. I."Predominance of precore mutations and clinical significance of basal core promoter mutations in chronic hepatitis B virus infection in Indonesia". Biomedical Reports 1, no. 4 (2013): 522-528. https://doi.org/10.3892/br.2013.106