Open Access

Biochemical and structural features of extracellular vesicle-binding RNA aptamers

  • Authors:
    • Kazuyoshi Murakami
    • Jing Zhao
    • Kazuhiko Yamasaki
    • Makoto Miyagishi
  • View Affiliations

  • Published online on: May 3, 2017     https://doi.org/10.3892/br.2017.899
  • Pages: 615-626
  • Copyright: © Murakami et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Extracellular vesicles are particles in mammalian body fluids that have attracted considerable attention as biomarkers for various diseases. In the present study, the authors isolated RNA aptamers with an affinity for extracellular vesicles from two library pools that encoded randomized sequences of different lengths. After the several rounds of selection, two conserved motifs are identified in the sequences that are obtained by next‑generation sequencing. Most of the sequences were predicted to adopt a secondary structure that consisted of a non‑conserved stem structure and a conserved loop sequence. Two minimal similar sequences are synthesized and confirmed the ability of these sequences to bind to extracellular vesicles. Circular dichroism spectroscopy and melting temperature analysis demonstrated that the aptamers were able to form a G‑quadruplex structure in their loop regions and these structures were stabilized by potassium ions. Consistent with these structural data, the affinity of each aptamer for extracellular vesicles was dependent on potassium ions. The aptamers that were identified may be useful molecular tools for the development of diagnostic methods that utilize body fluids, such as blood, saliva and urine.
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June-2017
Volume 6 Issue 6

Print ISSN: 2049-9434
Online ISSN:2049-9442

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Spandidos Publications style
Murakami K, Zhao J, Yamasaki K and Miyagishi M: Biochemical and structural features of extracellular vesicle-binding RNA aptamers. Biomed Rep 6: 615-626, 2017
APA
Murakami, K., Zhao, J., Yamasaki, K., & Miyagishi, M. (2017). Biochemical and structural features of extracellular vesicle-binding RNA aptamers. Biomedical Reports, 6, 615-626. https://doi.org/10.3892/br.2017.899
MLA
Murakami, K., Zhao, J., Yamasaki, K., Miyagishi, M."Biochemical and structural features of extracellular vesicle-binding RNA aptamers". Biomedical Reports 6.6 (2017): 615-626.
Chicago
Murakami, K., Zhao, J., Yamasaki, K., Miyagishi, M."Biochemical and structural features of extracellular vesicle-binding RNA aptamers". Biomedical Reports 6, no. 6 (2017): 615-626. https://doi.org/10.3892/br.2017.899