The pattern of IL‑24/mda‑7 and its cognate receptors expression following activation of human hepatic stellate cells

  • Authors:
    • Iman Jamhiri
    • Seyed Younes Hosseini
    • Davood Mehrabani
    • Zahra  Khodabandeh
    • Ramin Yaghobi
    • Razieh Dowran
    • Saber Zahri
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  • Published online on: June 21, 2017     https://doi.org/10.3892/br.2017.931
  • Pages: 173-178
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Abstract

Activation of hepatic stellate cells (HSCs) is the pivotal event during liver fibrosis. Interleukin (IL)‑24/mela­noma differentiation‑associated gene‑7 (mda‑7) has attracted attention in the pathophysiology of some diseases, while its role in activation/suppression of human HSCs is still unclear. It is important to elucidate whether the expression levels of the IL‑24/mda‑7 protein and its receptors in HSC cells are changed following activation. LX‑2 cells, a human hepatic stellate cell line were activated by a combination of leptin and serum starvation. The activation state was evaluated through measuring the mRNA expression of profibrotic molecules, collagen‑I, TIMP metalloproteinase inhibitor‑1 and transforming growth factor‑β. The expression of IL‑24/mda‑7 was assessed in mRNA and protein levels by reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) and ELISA methods, respectively. Hence, the amount of IL‑22R1 and IL‑20R2 subunit expression was also compared in activated and normal LX‑2 cells by RT‑qPCR. The expression level of IL‑24/mda‑7 and its cognate receptors was detectable both in the normal and activated LX‑2 cell line. Furthermore, in activated LX‑2, a significant increase of IL24 expression either on IL‑22R1 and IL‑20R2 subunits was also noticeable in comparison to normal cells. The activation state of LX‑2 cells caused significant changes of IL‑24/mda‑7 and its receptors expression. In addition, the elevation in IL‑24/mda‑7 during LX‑2 cell activation, suggested that IL‑24/mda‑7 and its cognate receptors serve a possible role in the development of the fibrosis process. Therefore, IL‑24/mda‑7 and relevant signaling pathways may be employed as a target for fibrosis treatment.
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August-2017
Volume 7 Issue 2

Print ISSN: 2049-9434
Online ISSN:2049-9442

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Spandidos Publications style
Jamhiri I, Hosseini S, Mehrabani D, Khodabandeh Z, Yaghobi R, Dowran R and Zahri S: The pattern of IL‑24/mda‑7 and its cognate receptors expression following activation of human hepatic stellate cells. Biomed Rep 7: 173-178, 2017
APA
Jamhiri, I., Hosseini, S., Mehrabani, D., Khodabandeh, Z., Yaghobi, R., Dowran, R., & Zahri, S. (2017). The pattern of IL‑24/mda‑7 and its cognate receptors expression following activation of human hepatic stellate cells. Biomedical Reports, 7, 173-178. https://doi.org/10.3892/br.2017.931
MLA
Jamhiri, I., Hosseini, S., Mehrabani, D., Khodabandeh, Z., Yaghobi, R., Dowran, R., Zahri, S."The pattern of IL‑24/mda‑7 and its cognate receptors expression following activation of human hepatic stellate cells". Biomedical Reports 7.2 (2017): 173-178.
Chicago
Jamhiri, I., Hosseini, S., Mehrabani, D., Khodabandeh, Z., Yaghobi, R., Dowran, R., Zahri, S."The pattern of IL‑24/mda‑7 and its cognate receptors expression following activation of human hepatic stellate cells". Biomedical Reports 7, no. 2 (2017): 173-178. https://doi.org/10.3892/br.2017.931