Overexpression of microRNA-15 increases the chemosensitivity of colon cancer cells to 5-fluorouracil and oxaliplatin by inhibiting the nuclear factor-κB signalling pathway and inducing apoptosis

  • Authors:
    • Lili Liu
    • Dan Wang
    • Ying Qiu
    • Hongyan Dong
    • Xuemei Zhan
  • View Affiliations

  • Published online on: December 22, 2017     https://doi.org/10.3892/etm.2017.5675
  • Pages: 2655-2660
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Abstract

Overcoming chemoresistance is a challenge in clinical treatment. It has been reported that microRNAs (miRNAs) are involved in regulating chemosensitivity. Therefore, the present study aimed to identify the effect and mechanism of miR‑15 on colon cancer chemotherapy. Reverse transcription‑quantitative polymerase chain reaction was performed to measure miR‑15 level sin62‑paired colon cancer and para‑cancerous colon tissues. The overexpression of miR‑15 in HCT116 cells was induced by transfection. The effect of miR‑15 on the chemosensitivity of colon cancer cells to 5‑fluorouracil (5‑FU) and Oxaliplatin (OX) was determined using a luminescent cell viability assay. Flow cytometry, dual‑luciferase assay and western blot analysis were used to determine the potential mechanism of miR‑15. The results suggested that the expression of miR‑15 was decreased in tumour tissues and that overexpression of miR‑15 increased the chemosensitivity of colon cancer cells to 5‑Fu and OX. miR‑15 promoted apoptosis in colon cancer cells treated with 5‑Fu and OX by inhibiting the expression of p50, which repressed the expression of B cell lymphoma‑2 and B cell lymphoma‑extra large; two direct target genes of nuclear factor‑κB with anti‑apoptotic functions. Thus, the current study demonstrated that miR‑15 increased the chemosensitivity of colon cancer cells to 5‑FU and OX by inhibiting the NF‑κB signalling pathway and inducing apoptosis.
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March-2018
Volume 15 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Liu L, Wang D, Qiu Y, Dong H and Zhan X: Overexpression of microRNA-15 increases the chemosensitivity of colon cancer cells to 5-fluorouracil and oxaliplatin by inhibiting the nuclear factor-κB signalling pathway and inducing apoptosis. Exp Ther Med 15: 2655-2660, 2018
APA
Liu, L., Wang, D., Qiu, Y., Dong, H., & Zhan, X. (2018). Overexpression of microRNA-15 increases the chemosensitivity of colon cancer cells to 5-fluorouracil and oxaliplatin by inhibiting the nuclear factor-κB signalling pathway and inducing apoptosis. Experimental and Therapeutic Medicine, 15, 2655-2660. https://doi.org/10.3892/etm.2017.5675
MLA
Liu, L., Wang, D., Qiu, Y., Dong, H., Zhan, X."Overexpression of microRNA-15 increases the chemosensitivity of colon cancer cells to 5-fluorouracil and oxaliplatin by inhibiting the nuclear factor-κB signalling pathway and inducing apoptosis". Experimental and Therapeutic Medicine 15.3 (2018): 2655-2660.
Chicago
Liu, L., Wang, D., Qiu, Y., Dong, H., Zhan, X."Overexpression of microRNA-15 increases the chemosensitivity of colon cancer cells to 5-fluorouracil and oxaliplatin by inhibiting the nuclear factor-κB signalling pathway and inducing apoptosis". Experimental and Therapeutic Medicine 15, no. 3 (2018): 2655-2660. https://doi.org/10.3892/etm.2017.5675