Effect of dihydroarteminin combined with siRNA targeting Notch1 on Notch1/c-Myc signaling in T-cell lymphoma cells

  • Authors:
    • Lanfen Huo
    • Wenwen Wei
    • Shaoling Wu
    • Xindong Zhao
    • Chunting Zhao
    • Hongguo Zhao
    • Lingjie Sun
  • View Affiliations

  • Published online on: January 23, 2018     https://doi.org/10.3892/etm.2018.5784
  • Pages: 3059-3065
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Abstract

The effectiveness of therapy combining dihydroartemisinin (DHA) and small interfering RNA targeting Notch1 (siNotch1) in T-cell lymphoma remains unknown. The present study explored the potential and possible mechanisms of combined dihydroarteminin, and siNotch1 therapy for T‑cell lymphoma. It was demonstrated that the viability rates of siRNA-DHA-treated cells was significantly suppressed in comparison with those in control cells, control siRNA cells, siRNA‑treated cells and DHA‑treated cells (P<0.01). Additionally, there was a significant increase in cell apoptosis of siRNA‑DHA‑treated cells in comparison with those of control cells, control siRNA cells, siRNA‑treated cells, DHA‑treated cells (P<0.05). Furthermore, Notch1 and c‑Myc mRNA and protein expression were decreased in siRNA‑DHA‑treated cells (P<0.05). The present study demonstrated that DHA combined with siNotch1 is able to suppress proliferation and promote apoptosis, and downregulate the expression of Notch1 and c‑Myc mRNA and protein in T‑cell lymphoma cells. Targeting Notch1/c‑Myc signaling with siRNA and DHA may represent a novel strategy for treating human T‑cell lymphoma.
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March-2018
Volume 15 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Huo L, Wei W, Wu S, Zhao X, Zhao C, Zhao H and Sun L: Effect of dihydroarteminin combined with siRNA targeting Notch1 on Notch1/c-Myc signaling in T-cell lymphoma cells. Exp Ther Med 15: 3059-3065, 2018
APA
Huo, L., Wei, W., Wu, S., Zhao, X., Zhao, C., Zhao, H., & Sun, L. (2018). Effect of dihydroarteminin combined with siRNA targeting Notch1 on Notch1/c-Myc signaling in T-cell lymphoma cells. Experimental and Therapeutic Medicine, 15, 3059-3065. https://doi.org/10.3892/etm.2018.5784
MLA
Huo, L., Wei, W., Wu, S., Zhao, X., Zhao, C., Zhao, H., Sun, L."Effect of dihydroarteminin combined with siRNA targeting Notch1 on Notch1/c-Myc signaling in T-cell lymphoma cells". Experimental and Therapeutic Medicine 15.3 (2018): 3059-3065.
Chicago
Huo, L., Wei, W., Wu, S., Zhao, X., Zhao, C., Zhao, H., Sun, L."Effect of dihydroarteminin combined with siRNA targeting Notch1 on Notch1/c-Myc signaling in T-cell lymphoma cells". Experimental and Therapeutic Medicine 15, no. 3 (2018): 3059-3065. https://doi.org/10.3892/etm.2018.5784