Mechanism of hydrogen peroxide-induced apoptosis of the human osteosarcoma cell line HS-Os-1

  • Authors:
    • Yasuhiro Ogawa
    • Toshiaki Takahashi
    • Toshihiro Kobayashi
    • Shinji Kariya
    • Akihito Nishioka
    • Hiroo Mizobuchi
    • Masataka Noguchi
    • Shinji Hamasato
    • Toshikazu Tani
    • Harumichi Seguchi
    • Shoji Yoshida
    • Hiroshi Sonobe
  • View Affiliations

  • Published online on: October 1, 2003     https://doi.org/10.3892/ijmm.12.4.459
  • Pages: 459-463
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

In our previous study, we examined radiation-induced ROS formation, oxidative DNA damage, early apoptotic changes, and mitochondrial membrane dysfunction in the human osteosarcoma cell line HS-Os-1, which was established from an osteoblastic tumor that arose in the left humerus of an 11-year-old girl and was already morphologically characterized in vitro and in vivo. We found that ROS formation and oxidative DNA damage were scarcely seen after irradiation of up to 30 Gy in these cells; that mitochondrial membrane potential was preserved; and that apoptotic changes were not demonstrated despite the relatively high-dose irradiation of 30 Gy. Based on these results, the radioresistance of the human osteosarcoma cell line HS-Os-1, was considered to arise, at least in part, from the low level of ROS formation following irradiation, which in turn may have resulted from the strong scavenging ability of the cells for free radicals, including hydroxyl radicals. Therefore, in this study, we examined the effect of exogenous hydrogen peroxide, which causes a potent oxidative stress and has been demonstrated to be a potent apoptosis-inducer in many kinds of cells. We found that addition of 1 or 10 mM hydrogen peroxide induced ROS formation, oxidative DNA damage, dysfunction of the mitochondrial membrane potential, and early apoptotic changes in the human osteosarcoma cell line HS-Os-1. We therefore concluded that intracellular ROS formation is involved in the hydrogen peroxide-induced apoptosis of HS-Os-1 cells.

Related Articles

Journal Cover

October 2003
Volume 12 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Ogawa Y, Takahashi T, Kobayashi T, Kariya S, Nishioka A, Mizobuchi H, Noguchi M, Hamasato S, Tani T, Seguchi H, Seguchi H, et al: Mechanism of hydrogen peroxide-induced apoptosis of the human osteosarcoma cell line HS-Os-1. Int J Mol Med 12: 459-463, 2003.
APA
Ogawa, Y., Takahashi, T., Kobayashi, T., Kariya, S., Nishioka, A., Mizobuchi, H. ... Sonobe, H. (2003). Mechanism of hydrogen peroxide-induced apoptosis of the human osteosarcoma cell line HS-Os-1. International Journal of Molecular Medicine, 12, 459-463. https://doi.org/10.3892/ijmm.12.4.459
MLA
Ogawa, Y., Takahashi, T., Kobayashi, T., Kariya, S., Nishioka, A., Mizobuchi, H., Noguchi, M., Hamasato, S., Tani, T., Seguchi, H., Yoshida, S., Sonobe, H."Mechanism of hydrogen peroxide-induced apoptosis of the human osteosarcoma cell line HS-Os-1". International Journal of Molecular Medicine 12.4 (2003): 459-463.
Chicago
Ogawa, Y., Takahashi, T., Kobayashi, T., Kariya, S., Nishioka, A., Mizobuchi, H., Noguchi, M., Hamasato, S., Tani, T., Seguchi, H., Yoshida, S., Sonobe, H."Mechanism of hydrogen peroxide-induced apoptosis of the human osteosarcoma cell line HS-Os-1". International Journal of Molecular Medicine 12, no. 4 (2003): 459-463. https://doi.org/10.3892/ijmm.12.4.459