Open Access

Humulus japonicus inhibits the progression of Alzheimer's disease in a APP/PS1 transgenic mouse model

  • Authors:
    • Tae-Shin Park
    • Young-Kyoung Ryu
    • Hye-Yeon Park
    • Jae Yun Kim
    • Jun Go
    • Jung-Ran Noh
    • Yong-Hoon Kim
    • Jung Hwan Hwang
    • Dong-Hee Choi
    • Won-Keun Oh
    • Chul-Ho Lee
    • Kyoung-Shim Kim
  • View Affiliations

  • Published online on: Wednesday, November 16, 2016
  • Pages:21-30 DOI: 10.3892/ijmm.2016.2804
  • Copyright: © Park et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Humulus japonicus Siebold & Zucc. (HJ) has traditionally been administered to patients with pulmonary disease, skin disease and hypertension in Korea, and it is considered to exert anti-inflammatory, antioxidant, antimicrobial and antimycobacterial effects. However, its effects against Alzheimer's disease (AD) have yet to be explored. Thus, this study was carried out to investigate whether HJ has a beneficial effect on the progression of AD in an animal model. A methanolic extract of HJ (500 mg/kg/day) was intragastrically administered to 5-month-old APP/PS1 transgenic (Tg-APP/PS1) mice for 2.5 months. Novel object recognition and Y-maze alteration tests were used to assess cognitive function, and an immunohistochemical assay was performed to assess amyloid β (Aβ)deposition, tau phosphorylation and gliosis. An in vitro assay using a microglial cell line was also performed to investigate the anti-inflammatory effects of HJ. Our results revealed that HJ significantly decreased the mRNA and protein expression levels of tumor necrosis factor-α (TNF‑α), interleukin (IL)-1β, IL-6 and inducible nitric oxide synthase (iNOS) induced by lipopolysaccharide in the microglial cell line. The administration of HJ for 2 months improved the cognitive function of Tg-APP/PS1 mice. HJ notably reduced the area occupied by Aβ and neurofibrillary tangles, and the number of activated astrocytes and microglia in the cortex of Tg-APP/PS1 mice. The findings of our study suggest that HJ has the therapeutic potential to inhibit the progression of AD and to improve cognitive deterioration in Tg-APP/PS1 mice.

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January 2017
Volume 39 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

2015 Impact Factor: 2.348
Ranked #22/123 Medicine Research and Experimental
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APA
Park, T., Ryu, Y., Park, H., Kim, J.Y., Go, J., Noh, J. ... Kim, K. (2017). Humulus japonicus inhibits the progression of Alzheimer's disease in a APP/PS1 transgenic mouse model. International Journal of Molecular Medicine, 39, 21-30. http://dx.doi.org/10.3892/ijmm.2016.2804
MLA
Park, T., Ryu, Y., Park, H., Kim, J. Y., Go, J., Noh, J., Kim, Y., Hwang, J. H., Choi, D., Oh, W., Lee, C., Kim, K."Humulus japonicus inhibits the progression of Alzheimer's disease in a APP/PS1 transgenic mouse model". International Journal of Molecular Medicine 39.1 (2017): 21-30.
Chicago
Park, T., Ryu, Y., Park, H., Kim, J. Y., Go, J., Noh, J., Kim, Y., Hwang, J. H., Choi, D., Oh, W., Lee, C., Kim, K."Humulus japonicus inhibits the progression of Alzheimer's disease in a APP/PS1 transgenic mouse model". International Journal of Molecular Medicine 39, no. 1 (2017): 21-30. http://dx.doi.org/10.3892/ijmm.2016.2804