Open Access

MicroRNA-327 regulates cardiac hypertrophy and fibrosis induced by pressure overload

  • Authors:
    • Yue Ji
    • Ming Qiu
    • Yejiao Shen
    • Li Gao
    • Yaqing Wang
    • Wei Sun
    • Xinli Li
    • Yan Lu
    • Xiangqing Kong
  • View Affiliations

  • Published online on: January 25, 2018     https://doi.org/10.3892/ijmm.2018.3428
  • Pages: 1909-1916
  • Copyright: © Ji et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

MicroRNA (miRNA/miR) dysregulation has been reported to be fundamental in the development and progression of cardiac hypertrophy and fibrosis. In the present study, miR-327 levels in fibroblasts were increased in response to cardiac hypertrophy induced by transverse aortic constriction with prominent cardiac fibrosis, particularly when compared with the levels in unstressed cardiomyocytes. In neonatal rat cardiac fibroblasts, induced expression of miR-327 upregulated fibrosis-associated gene expression and activated angiotensin II-induced differentiation into myofibroblasts, as assessed via α-smooth muscle actin staining. By contrast, miR-327 knockdown mitigated angiotensin II-induced differentiation. Cardiac fibroblast proliferation was not affected under either condition. In a mouse model subjected to transverse aortic constriction, miR-327 knockdown through tail-vein injection reduced the development of cardiac fibrosis and ventricular dysfunction. miR-327 was demonstrated to target integrin β3 and diminish the activation of cardiac fibroblasts. Thus, the present study supports the use of miR-327 as a therapeutic target in the reduction of cardiac fibrosis.
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April-2018
Volume 41 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Copy and paste a formatted citation
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Spandidos Publications style
Ji Y, Qiu M, Shen Y, Gao L, Wang Y, Sun W, Li X, Lu Y and Kong X: MicroRNA-327 regulates cardiac hypertrophy and fibrosis induced by pressure overload. Int J Mol Med 41: 1909-1916, 2018
APA
Ji, Y., Qiu, M., Shen, Y., Gao, L., Wang, Y., Sun, W. ... Kong, X. (2018). MicroRNA-327 regulates cardiac hypertrophy and fibrosis induced by pressure overload. International Journal of Molecular Medicine, 41, 1909-1916. https://doi.org/10.3892/ijmm.2018.3428
MLA
Ji, Y., Qiu, M., Shen, Y., Gao, L., Wang, Y., Sun, W., Li, X., Lu, Y., Kong, X."MicroRNA-327 regulates cardiac hypertrophy and fibrosis induced by pressure overload". International Journal of Molecular Medicine 41.4 (2018): 1909-1916.
Chicago
Ji, Y., Qiu, M., Shen, Y., Gao, L., Wang, Y., Sun, W., Li, X., Lu, Y., Kong, X."MicroRNA-327 regulates cardiac hypertrophy and fibrosis induced by pressure overload". International Journal of Molecular Medicine 41, no. 4 (2018): 1909-1916. https://doi.org/10.3892/ijmm.2018.3428