Selenium improves stem cell potency by stimulating the proliferation and active migration of 3T3-L1 preadipocytes

  • Authors:
    • Shin-Hyung Park
    • Jeong-Hwan Kim
    • Soo-Wan Nam
    • Byung-Woo Kim
    • Gi-Young Kim
    • Wun-Jae Kim
    • Yung Hyun Choi
  • View Affiliations

  • Published online on: November 15, 2013     https://doi.org/10.3892/ijo.2013.2182
  • Pages: 336-342
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Abstract

Selenium is a trace nutrient element that protects cells against oxidative damage. In this study, the potential of selenium to improve stem cell potency through active proliferation and migration of 3T3-L1 preadipocytes was investigated, together with the underlying molecular mechanisms. The results indicated that selenium applied for 24 h stimulated cell proliferation up to 20% compared to untreated control cells. Selenium induced the expression of cyclin-dependent kinase (CDK) 1 and CDK2, which are known to regulate G2/M progression, and significantly downregulated the CDK inhibitors p21 and p27. Selenium also activated the expression of the phosphatidylinositol 3-kinase (PI3K)/Akt pathway, as well as extracellular signal-regulated kinase (ERK). Although LY294002, an inhibitor of PI3K, significantly inhibited the selenium-induced cell proliferation of the 3T3-L1 preadipocytes, PD98059, an inhibitor of ERK, did not affect selenium-induced active proliferation. These results clearly indicate that selenium stimulated cell proliferation through cell cycle progression and PI3K/Akt activation, but not through ERK activation. Furthermore, selenium increased 3T3-L1 cell migration, which was associated with the induction of matrix metalloproteinase (MMP)-2 and MMP-9. Taken together, the current findings suggest that selenium can stimulate stem cell potency by increasing the proliferation and active migration of 3T3-L1 cells.
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2014-January
Volume 44 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Park S, Kim J, Nam S, Kim B, Kim G, Kim W and Choi YH: Selenium improves stem cell potency by stimulating the proliferation and active migration of 3T3-L1 preadipocytes. Int J Oncol 44: 336-342, 2014
APA
Park, S., Kim, J., Nam, S., Kim, B., Kim, G., Kim, W., & Choi, Y.H. (2014). Selenium improves stem cell potency by stimulating the proliferation and active migration of 3T3-L1 preadipocytes. International Journal of Oncology, 44, 336-342. https://doi.org/10.3892/ijo.2013.2182
MLA
Park, S., Kim, J., Nam, S., Kim, B., Kim, G., Kim, W., Choi, Y. H."Selenium improves stem cell potency by stimulating the proliferation and active migration of 3T3-L1 preadipocytes". International Journal of Oncology 44.1 (2014): 336-342.
Chicago
Park, S., Kim, J., Nam, S., Kim, B., Kim, G., Kim, W., Choi, Y. H."Selenium improves stem cell potency by stimulating the proliferation and active migration of 3T3-L1 preadipocytes". International Journal of Oncology 44, no. 1 (2014): 336-342. https://doi.org/10.3892/ijo.2013.2182