Open Access

miR‑223‑3p regulates cell growth and apoptosis via FBXW7 suggesting an oncogenic role in human testicular germ cell tumors

  • Authors:
    • Jikai Liu
    • Hao Shi
    • Xidan Li
    • Gang Chen
    • Catharina Larsson
    • Weng-Onn Lui
  • View Affiliations

  • Published online on: Thursday, December 15, 2016
  • Pages:356-364 DOI: 10.3892/ijo.2016.3807
  • Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

miR‑223‑3p is deregulated in several tumor types and plays an important role in tumorigenesis and progression. However, its role in the pathogenesis of testicular germ cell tumor (TGCT) remains uncharacterized. We previously demonstrated that miR‑223‑3p expression was increased in TGCTs compared with normal testes (NT), suggesting that miR‑223‑3p may have an oncogenic role in TGCT. Using published dataset and The Cancer Genome Atlas database, we validated higher miR‑223‑3p expression in TGCTs than NT, and found a negative correlation between miR-223-3p and FBXW7 mRNA expression levels. Using both gain- and loss-of-function experiments, we show that miR‑223‑3p regulates FBXW7 protein expression, cell growth and apoptosis in TGCT cell lines. Additionally, we demonstrate that ectopic expression of the full-length coding sequence of FBXW7 could rescue the cell growth and apoptotic effects mediated by miR‑223‑3p. Our findings suggest an oncogenic role for miR‑223‑3p in TGCT, which promotes cell growth and inhibits apoptosis through repression of FBXW7.

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February 2017
Volume 50 Issue 2

Print ISSN: 1019-6439
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APA
Liu, J., Shi, H., Li, X., Chen, G., Larsson, C., & Lui, W. (2017). miR‑223‑3p regulates cell growth and apoptosis via FBXW7 suggesting an oncogenic role in human testicular germ cell tumors. International Journal of Oncology, 50, 356-364. http://dx.doi.org/10.3892/ijo.2016.3807
MLA
Liu, J., Shi, H., Li, X., Chen, G., Larsson, C., Lui, W."miR‑223‑3p regulates cell growth and apoptosis via FBXW7 suggesting an oncogenic role in human testicular germ cell tumors". International Journal of Oncology 50.2 (2017): 356-364.
Chicago
Liu, J., Shi, H., Li, X., Chen, G., Larsson, C., Lui, W."miR‑223‑3p regulates cell growth and apoptosis via FBXW7 suggesting an oncogenic role in human testicular germ cell tumors". International Journal of Oncology 50, no. 2 (2017): 356-364. http://dx.doi.org/10.3892/ijo.2016.3807