Open Access

Upregulation of miR-146a by YY1 depletion correlates with delayed progression of prostate cancer

  • Authors:
    • Yeqing Huang
    • Tao Tao
    • Chunhui Liu
    • Han Guan
    • Guangyuan Zhang
    • Zhixin Ling
    • Lei Zhang
    • Kai Lu
    • Shuqiu Chen
    • Bin Xu
    • Ming Chen
  • View Affiliations

  • Published online on: Thursday, January 5, 2017
  • Pages:421-431 DOI: 10.3892/ijo.2017.3840
  • Copyright: © Huang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Previously published studies explained that the excessive expression of miR-146a influences the prostate cancer (PCa) cells in terms of apoptosis, progression, and viability. Although miR-146a acts as a tumor suppressor, current knowledge on the molecular mechanisms that controls its expression in PCa is limited. In this study, gene set enrichment analysis (GSEA) showed negatively enriched expression of miR-146a target gene sets and positively enriched expression of gene sets suppressed by the enhancer of zeste homolog 2 (EZH2) after YY1 depletion in PCa cells. The current results demonstrated that the miR-146a levels in PCa tissues with high Gleason scores (>7) are significantly lower than those in PCa tissues with low Gleason scores (≤7), which were initially observed in the clinical specimens. An inverse relationship between YY1 and miR-146a expression was also observed. Experiments indicated the decrease in cell viability, proliferation, and promoting apoptosis after YY1 depletion, while through inhibiting miR-146a could alleviate the negative effect brought by YY1 depletion. We detected the reversed adjustment of YY1 to accommodate miR-146a transcriptions. On the basis of YY1 depletion, we determined that the expression of miR-146a increased after EZH2 knockdown. We validated the combination of YY1 and its interaction with EZH2 at the miR-146a promoter binding site, thereby prohibiting the transcriptional activity of miR-146a in PCa cells. Our results suggested that YY1 depletion repressed PCa cell viability and proliferation and induced apoptosis at least in a miR-146a-assisted manner.

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February 2017
Volume 50 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Copy and paste a formatted citation
APA
Huang, Y., Tao, T., Liu, C., Guan, H., Zhang, G., Ling, Z. ... Chen, M. (2017). Upregulation of miR-146a by YY1 depletion correlates with delayed progression of prostate cancer. International Journal of Oncology, 50, 421-431. http://dx.doi.org/10.3892/ijo.2017.3840
MLA
Huang, Y., Tao, T., Liu, C., Guan, H., Zhang, G., Ling, Z., Zhang, L., Lu, K., Chen, S., Xu, B., Chen, M."Upregulation of miR-146a by YY1 depletion correlates with delayed progression of prostate cancer". International Journal of Oncology 50.2 (2017): 421-431.
Chicago
Huang, Y., Tao, T., Liu, C., Guan, H., Zhang, G., Ling, Z., Zhang, L., Lu, K., Chen, S., Xu, B., Chen, M."Upregulation of miR-146a by YY1 depletion correlates with delayed progression of prostate cancer". International Journal of Oncology 50, no. 2 (2017): 421-431. http://dx.doi.org/10.3892/ijo.2017.3840