Open Access

Effects of 17-AAG on the cell cycle and apoptosis of H446 cells and the associated mechanisms

  • Authors:
    • Xuerong Zhao
    • Jianping Wang
    • Lijun Xiao
    • Qian Xu
    • Enhong Zhao
    • Xin Zheng
    • Huachuan Zheng
    • Shuang Zhao
    • Shi Ding
  • View Affiliations

  • Published online on: June 6, 2016     https://doi.org/10.3892/mmr.2016.5365
  • Pages: 1067-1074
  • Copyright: © Zhao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

As a heat shock protein 90 inhibitor, 17-allylamino-17‑demethoxygeldanamycin (17-AAG) has been studied in numerous types of cancer, however the effects of 17-AAG on apoptosis and the cell cycle of H446 cells remain unclear. In the current study, the MTT method was used to evaluate the inhibitory effects of different durations and doses of 17‑AAG treatment on the proliferation of H446 cells. The cells were stained with Annexin-fluorescein isothiocyanate/propidium iodide and measured by flow cytometry, and the gene and protein expression levels of signal transducer and activator of transcription 3 (STAT3), survivin, cyclin D1, cyt‑C, caspase 9 and caspase 3 were determined by reverse transcription‑quantitative polymerase chain reaction and western blot analysis. The results indicated that with treatment with 1.25‑20 mg/l 17‑AAG for 24 and 48 h, significant inhibition of H446 cell proliferation was observed in a time‑ and dose‑dependent manner. With treatment of 3.125, 6.25 and 12.5 mg/l 17‑AAG for 48 h, significant apoptosis and cell cycle arrest was observed. The results indicated that the gene and protein expression levels of STAT3, survivin and cyclin D1 were downregulated, and cyt‑C, caspase 9 and caspase 3 were upregulated by 17‑AAG in a dose-dependent manner when the cells were treated with 3.125 and 6.25 mg/l 17-AAG for 48 h. The results indicated that 17‑AAG is able to inhibit the cell proliferation, induce apoptosis and G2/M arrest and downregulate the gene and protein expression levels of STAT3, survivin and cyclin D1, and upregulate gene and protein expression of cyt‑C, caspase 9, caspase 3.

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August-2016
Volume 14 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Zhao X, Wang J, Xiao L, Xu Q, Zhao E, Zheng X, Zheng H, Zhao S and Ding S: Effects of 17-AAG on the cell cycle and apoptosis of H446 cells and the associated mechanisms. Mol Med Rep 14: 1067-1074, 2016
APA
Zhao, X., Wang, J., Xiao, L., Xu, Q., Zhao, E., Zheng, X. ... Ding, S. (2016). Effects of 17-AAG on the cell cycle and apoptosis of H446 cells and the associated mechanisms. Molecular Medicine Reports, 14, 1067-1074. https://doi.org/10.3892/mmr.2016.5365
MLA
Zhao, X., Wang, J., Xiao, L., Xu, Q., Zhao, E., Zheng, X., Zheng, H., Zhao, S., Ding, S."Effects of 17-AAG on the cell cycle and apoptosis of H446 cells and the associated mechanisms". Molecular Medicine Reports 14.2 (2016): 1067-1074.
Chicago
Zhao, X., Wang, J., Xiao, L., Xu, Q., Zhao, E., Zheng, X., Zheng, H., Zhao, S., Ding, S."Effects of 17-AAG on the cell cycle and apoptosis of H446 cells and the associated mechanisms". Molecular Medicine Reports 14, no. 2 (2016): 1067-1074. https://doi.org/10.3892/mmr.2016.5365