Mechanical stress influences the viability and morphology of human parametrial ligament fibroblasts

  • Authors:
    • Ming Hu
    • Li Hong
    • Shasha Hong
    • Jie Min
    • Yang Zhao
    • Qing Yang
    • Qifan Zhang
    • Jianming Tang
    • Yang Li
  • View Affiliations

  • Published online on: December 15, 2016     https://doi.org/10.3892/mmr.2016.6052
  • Pages: 853-858
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Abstract

The present study aimed to investigate damage to human parametrial ligament fibroblasts by detecting cell proliferation, cytoskeletal structure, cellular alterations and senescence. Uterosacral and cardinal ligaments were obtained from 10 patients with cervical intraepithelial neoplasia grade II‑III, who had received total vaginal hysterectomies, and fibroblasts were derived from this tissue. Fibroblasts were stretched using a four‑point bending system with a force of 0 (control), 1,333 µ strain (1 mm) or 5,333 µ strain (4 mm) for 4 h. The present study revealed that mechanical force significantly reduced cell proliferation and increased cell senescence. As mechanical force increased, the mitochondria of fibroblasts began to exhibit vacuolization, and the cell cytoskeleton began to depolymerize and rearrange. In conclusion, the present study demonstrated that mechanical forces within a certain range may induce cell damage via mitochondrial injury, cytoskeletal alterations and increased cell senescence, resulting in decreased cell viability of pelvic fibroblasts.
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February-2017
Volume 15 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Hu M, Hong L, Hong S, Min J, Zhao Y, Yang Q, Zhang Q, Tang J and Li Y: Mechanical stress influences the viability and morphology of human parametrial ligament fibroblasts. Mol Med Rep 15: 853-858, 2017
APA
Hu, M., Hong, L., Hong, S., Min, J., Zhao, Y., Yang, Q. ... Li, Y. (2017). Mechanical stress influences the viability and morphology of human parametrial ligament fibroblasts. Molecular Medicine Reports, 15, 853-858. https://doi.org/10.3892/mmr.2016.6052
MLA
Hu, M., Hong, L., Hong, S., Min, J., Zhao, Y., Yang, Q., Zhang, Q., Tang, J., Li, Y."Mechanical stress influences the viability and morphology of human parametrial ligament fibroblasts". Molecular Medicine Reports 15.2 (2017): 853-858.
Chicago
Hu, M., Hong, L., Hong, S., Min, J., Zhao, Y., Yang, Q., Zhang, Q., Tang, J., Li, Y."Mechanical stress influences the viability and morphology of human parametrial ligament fibroblasts". Molecular Medicine Reports 15, no. 2 (2017): 853-858. https://doi.org/10.3892/mmr.2016.6052