Open Access

Hepatic differentiation of mouse bone marrow‑derived mesenchymal stem cells using a novel 3D culture system

  • Authors:
    • Qiong Wu
    • Jing Tang
    • Yi Li
    • Li Li
    • Yujia Wang
    • Ji Bao
    • Hong Bu
  • View Affiliations

  • Published online on: October 19, 2017     https://doi.org/10.3892/mmr.2017.7818
  • Pages: 9473-9479
  • Copyright: © Wu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The development of novel culture systems that mimic the in vivo microenvironment may be beneficial for inducing the differentiation of stem cells and promoting liver function. In the present study, spheroid cultures and decellularized liver scaffolds (DLSs) were utilized to obtain differentiated hepatocyte‑like cells. Mouse bone marrow (BM)‑derived mesenchymal stem cells (MSCs) self‑aggregated into spheroids under low‑attachment conditions and implanted into the DLSs via a negative pressure suction device. The Albp‑ZsGreen adenoviral vector was utilized for real‑time monitoring of hepatocyte‑like cell differentiation. To detect the differentiation stages of the MSCs, immunostaining of hepatocyte markers and functional analysis was performed. Compared with traditional 2D monolayer induction, mouse BM‑MSCs spheroids and DLSs in 3D culture generated greater yields of mature, differentiated hepatocytes. In conclusion, this 3D culture system may provide a strategy for generating hepatocyte‑like cells for portable liver micro‑organs, and aid clinical hepatocyte transplantation and liver tissue engineering.
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December-2017
Volume 16 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Wu Q, Tang J, Li Y, Li L, Wang Y, Bao J and Bu H: Hepatic differentiation of mouse bone marrow‑derived mesenchymal stem cells using a novel 3D culture system. Mol Med Rep 16: 9473-9479, 2017
APA
Wu, Q., Tang, J., Li, Y., Li, L., Wang, Y., Bao, J., & Bu, H. (2017). Hepatic differentiation of mouse bone marrow‑derived mesenchymal stem cells using a novel 3D culture system. Molecular Medicine Reports, 16, 9473-9479. https://doi.org/10.3892/mmr.2017.7818
MLA
Wu, Q., Tang, J., Li, Y., Li, L., Wang, Y., Bao, J., Bu, H."Hepatic differentiation of mouse bone marrow‑derived mesenchymal stem cells using a novel 3D culture system". Molecular Medicine Reports 16.6 (2017): 9473-9479.
Chicago
Wu, Q., Tang, J., Li, Y., Li, L., Wang, Y., Bao, J., Bu, H."Hepatic differentiation of mouse bone marrow‑derived mesenchymal stem cells using a novel 3D culture system". Molecular Medicine Reports 16, no. 6 (2017): 9473-9479. https://doi.org/10.3892/mmr.2017.7818