Open Access

MicroRNA‑133b inhibits cell migration and invasion by targeting matrix metalloproteinase 14 in glioblastoma

  • Authors:
    • Liang Chang
    • Xuhui Lei
    • Yu Qin
    • Xuexin Zhang
    • Hua Jin
    • Chao Wang
    • Xin Wang
    • Guofu Li
    • Chunlei Tan
    • Jun Su
  • View Affiliations

  • Published online on: September 2, 2015     https://doi.org/10.3892/ol.2015.3657
  • Pages: 2781-2786
  • Copyright: © Chang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Increasing evidence has suggested that microRNA‑133b (miR‑133b) is important in regulating the genesis of different types of cancer. However, the effects and the underlying mechanisms of miR‑133b in the development of glioblastoma (GBM) remain largely unknown. The aim of the present study was to investigate the role of miR‑133b in GBM and to determine the molecular mechanisms underlying its action. Reverse transcription‑quantitative polymerase chain reaction was used to measure the expression levels of miR‑133b in 21 human GBM samples and 9 normal brain tissue samples. A wound healing assay, and Transwell migration and invasion assays were used to evaluate the effects of miR‑133b on cell migration and invasion. Western blotting and a luciferase reporter assay were used to identify the target genes of miR‑133b. It was found that miR‑133b suppressed GBM cell migration and invasion, and matrix metalloproteinase 14 (MMP14) was identified as a direct target gene. In conclusion, miR‑133b may suppress GBM migration and invasion through directly targeting MMP14, highlighting its potential as a novel agent for the treatment of GBM invasion.
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November-2015
Volume 10 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Chang L, Lei X, Qin Y, Zhang X, Jin H, Wang C, Wang X, Li G, Tan C, Su J, Su J, et al: MicroRNA‑133b inhibits cell migration and invasion by targeting matrix metalloproteinase 14 in glioblastoma. Oncol Lett 10: 2781-2786, 2015
APA
Chang, L., Lei, X., Qin, Y., Zhang, X., Jin, H., Wang, C. ... Su, J. (2015). MicroRNA‑133b inhibits cell migration and invasion by targeting matrix metalloproteinase 14 in glioblastoma. Oncology Letters, 10, 2781-2786. https://doi.org/10.3892/ol.2015.3657
MLA
Chang, L., Lei, X., Qin, Y., Zhang, X., Jin, H., Wang, C., Wang, X., Li, G., Tan, C., Su, J."MicroRNA‑133b inhibits cell migration and invasion by targeting matrix metalloproteinase 14 in glioblastoma". Oncology Letters 10.5 (2015): 2781-2786.
Chicago
Chang, L., Lei, X., Qin, Y., Zhang, X., Jin, H., Wang, C., Wang, X., Li, G., Tan, C., Su, J."MicroRNA‑133b inhibits cell migration and invasion by targeting matrix metalloproteinase 14 in glioblastoma". Oncology Letters 10, no. 5 (2015): 2781-2786. https://doi.org/10.3892/ol.2015.3657