CD147 promotes the proliferation, invasiveness, migration and angiogenesis of human lung carcinoma cells

  • Authors:
    • Shaoxing Yang
    • Fei Qi
    • Chuanhao Tang
    • Hong Wang
    • Haifeng Qin
    • Xiaoyan Li
    • Jianjie Li
    • Weixia Wang
    • Changyun Zhao
    • Hongjun Gao
  • View Affiliations

  • Published online on: December 14, 2016     https://doi.org/10.3892/ol.2016.5502
  • Pages: 898-904
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Cluster of differentiation (CD) 147 is a transmembrane glycoprotein that is highly expressed at the tumor cell surface, which stimulates fibroblasts to produce a large number of matrix metalloproteinases and promotes tumor invasion and metastasis and tumor‑induced angiogenesis. The present study investigated the functions and the role of CD147 in the human lung carcinoma A549 cell line. The present study constructed expression and interference [small interfering (si) RNA] lentiviral vectors of CD147, which established stable overexpression and low expression of CD147 in the A549 cell line, named A549‑CD147 and A549‑siCD147, respectively. The differences in biological features between various levels of CD147 expression in A549 cells was investigated by cell counting kit‑8 (CCK‑8), Transwell, scratch and lumen formation assays. The results of the CCK‑8 assay revealed that A549‑CD147 cell proliferation was significantly increased and A549‑siCD147 cell proliferation was decreased compared with the control groups. The A549‑CD147 cells had the largest number of cells penetrating the Matrigel in the Transwell assay, which indicates that upregulation of CD147 expression increases the infiltration capacity of cells. The scratch assay revealed that A549‑CD147 cells have the highest capacity for migration, while A549‑siCD147 cells have the lowest. Quantitative polymerase chain reaction and western blot analysis demonstrated that vascular endothelial growth factor (VEGF) expression was proportional to the expression level of CD147 at the mRNA and protein level. The lumen formation assay revealed that the number of vessel lumens that human umbilical vein endothelial cells formed in the A549‑CD147 cell supernatant was increased compared with the A549‑siCD147 cells. Collectively, the present results suggest that CD147 is important in the promotion of lung carcinoma cell proliferation, invasion and metastasis and the upregulation of VEGF, which stimulates the angiogenesis of lung carcinoma. In conclusion, CD147 may be a potential target in the treatment of lung carcinoma.
View Figures
View References

Related Articles

Journal Cover

February-2017
Volume 13 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Yang S, Qi F, Tang C, Wang H, Qin H, Li X, Li J, Wang W, Zhao C, Gao H, Gao H, et al: CD147 promotes the proliferation, invasiveness, migration and angiogenesis of human lung carcinoma cells. Oncol Lett 13: 898-904, 2017
APA
Yang, S., Qi, F., Tang, C., Wang, H., Qin, H., Li, X. ... Gao, H. (2017). CD147 promotes the proliferation, invasiveness, migration and angiogenesis of human lung carcinoma cells. Oncology Letters, 13, 898-904. https://doi.org/10.3892/ol.2016.5502
MLA
Yang, S., Qi, F., Tang, C., Wang, H., Qin, H., Li, X., Li, J., Wang, W., Zhao, C., Gao, H."CD147 promotes the proliferation, invasiveness, migration and angiogenesis of human lung carcinoma cells". Oncology Letters 13.2 (2017): 898-904.
Chicago
Yang, S., Qi, F., Tang, C., Wang, H., Qin, H., Li, X., Li, J., Wang, W., Zhao, C., Gao, H."CD147 promotes the proliferation, invasiveness, migration and angiogenesis of human lung carcinoma cells". Oncology Letters 13, no. 2 (2017): 898-904. https://doi.org/10.3892/ol.2016.5502