Open Access

MicroRNA‑193a‑3p inhibits cell proliferation in prostate cancer by targeting cyclin D1

  • Authors:
    • Yunfu Liu
    • Xin Xu
    • Xianglai Xu
    • Shiqi Li
    • Zhen Liang
    • Zhenghui Hu
    • Jian Wu
    • Yi Zhu
    • Xiaodong Jin
    • Xiao Wang
    • Yiwei Lin
    • Hong Chen
    • Yeqing Mao
    • Jindan Luo
    • Xiangyi Zheng
    • Liping Xie
  • View Affiliations

  • Published online on: September 1, 2017     https://doi.org/10.3892/ol.2017.6865
  • Pages: 5121-5128
  • Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

MicroRNAs (miRNAs) are small non‑coding RNAs that affect various biological processes by altering the expression of a target gene. An miRNA microarray analysis has previously revealed a significant decrease in miR‑193a‑3p levels in prostate cancer tissues compared with that in their benign prostate hyperplasia counterparts. However, the role of miR‑193a‑3p has yet to be elucidated. In the present study, reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) was used to evaluate the expression levels of miR‑193a‑3p in two human prostate cancer cell lines. Forced overexpression of miR‑193a‑3p was established by transfecting mimics into DU‑145 and PC3 cell lines. Cell proliferation and the cell cycle were assessed using a cell viability assay, flow cytometry and a colony formation assay. In addition, the target gene of miR‑193a‑3p was determined by a luciferase assay, RT‑qPCR and western blot analysis. The regulation of the cell cycle by miR‑193a‑3p was also evaluated by western blotting. The results demonstrated that miR‑193a‑3p expression levels were lower in prostate cancer cell lines as compared with the RWPE normal prostate epithelium cell line. Subsequent gain‑of‑function studies revealed that stable miR‑193a‑3p transfection inhibited cell viability, proliferation and colony formation, and induced G1 phase arrest in prostate cancer cells. A luciferase assay and western blot analysis identified cyclin D1 (CCND1) as a direct target gene of miR‑193a‑3p. In addition, the forced expression of CCND1 was able to counter the inhibitory effects of miR‑193a‑3p transfection in the prostate cancer cells. In summary, the results suggest that miR‑193a‑3p may inhibit the viability, proliferation and survival of prostate cancer cells by regulating the expression profile of CCND1, and that miR‑193a‑3p may be a novel therapeutic biomarker for prostate cancer.
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November-2017
Volume 14 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Copy and paste a formatted citation
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Spandidos Publications style
Liu Y, Xu X, Xu X, Li S, Liang Z, Hu Z, Wu J, Zhu Y, Jin X, Wang X, Wang X, et al: MicroRNA‑193a‑3p inhibits cell proliferation in prostate cancer by targeting cyclin D1. Oncol Lett 14: 5121-5128, 2017
APA
Liu, Y., Xu, X., Xu, X., Li, S., Liang, Z., Hu, Z. ... Xie, L. (2017). MicroRNA‑193a‑3p inhibits cell proliferation in prostate cancer by targeting cyclin D1. Oncology Letters, 14, 5121-5128. https://doi.org/10.3892/ol.2017.6865
MLA
Liu, Y., Xu, X., Xu, X., Li, S., Liang, Z., Hu, Z., Wu, J., Zhu, Y., Jin, X., Wang, X., Lin, Y., Chen, H., Mao, Y., Luo, J., Zheng, X., Xie, L."MicroRNA‑193a‑3p inhibits cell proliferation in prostate cancer by targeting cyclin D1". Oncology Letters 14.5 (2017): 5121-5128.
Chicago
Liu, Y., Xu, X., Xu, X., Li, S., Liang, Z., Hu, Z., Wu, J., Zhu, Y., Jin, X., Wang, X., Lin, Y., Chen, H., Mao, Y., Luo, J., Zheng, X., Xie, L."MicroRNA‑193a‑3p inhibits cell proliferation in prostate cancer by targeting cyclin D1". Oncology Letters 14, no. 5 (2017): 5121-5128. https://doi.org/10.3892/ol.2017.6865