Open Access

Metformin inhibits the migration and invasion of esophageal squamous cell carcinoma cells by downregulating the protein kinase B signaling pathway

  • Authors:
    • Yindi He
    • Xiaojun Tan
    • Hui Hu
    • Qinjia Wang
    • Xi Hu
    • Xianbin Cai
    • Yinghong Guan
    • Binming Chen
    • Xubin Jing
  • View Affiliations

  • Published online on: December 28, 2017     https://doi.org/10.3892/ol.2017.7699
  • Pages: 2939-2945
  • Copyright: © He et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Previous studies have suggested that metformin, a biguanide family member widely used as an oral antidiabetic drug, may inhibit proliferation and induce apoptosis in certain types of cancer cell. However, the molecular mechanisms underlying metformin‑associated anticancer effects, and in particular antimetastatic effects, remain to be fully understood. The present study assessed the efficacy of metformin in inhibiting the migration and invasion of the esophageal carcinoma cell line EC109, and evaluated the effect of metformin on the protein kinase B (AKT) signaling pathway. EC109 cells were treated with 0, 5, 10 or 20 mM metformin during the logarithmic growth phase. A Transwell assay and western blot analysis revealed that metformin inhibited the migration and invasion of EC109 cells, nuclear factor‑κB activation, matrix metallopeptidase 9 and N‑cadherin expression in a phosphorylated‑AKT dependent manner. These results suggested that metformin inhibits the migration and invasion of human esophageal carcinoma cells by suppressing AKT phosphorylation and regulating the expression of migration‑ and invasion‑associated genes.
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March-2018
Volume 15 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
He Y, Tan X, Hu H, Wang Q, Hu X, Cai X, Guan Y, Chen B and Jing X: Metformin inhibits the migration and invasion of esophageal squamous cell carcinoma cells by downregulating the protein kinase B signaling pathway. Oncol Lett 15: 2939-2945, 2018
APA
He, Y., Tan, X., Hu, H., Wang, Q., Hu, X., Cai, X. ... Jing, X. (2018). Metformin inhibits the migration and invasion of esophageal squamous cell carcinoma cells by downregulating the protein kinase B signaling pathway. Oncology Letters, 15, 2939-2945. https://doi.org/10.3892/ol.2017.7699
MLA
He, Y., Tan, X., Hu, H., Wang, Q., Hu, X., Cai, X., Guan, Y., Chen, B., Jing, X."Metformin inhibits the migration and invasion of esophageal squamous cell carcinoma cells by downregulating the protein kinase B signaling pathway". Oncology Letters 15.3 (2018): 2939-2945.
Chicago
He, Y., Tan, X., Hu, H., Wang, Q., Hu, X., Cai, X., Guan, Y., Chen, B., Jing, X."Metformin inhibits the migration and invasion of esophageal squamous cell carcinoma cells by downregulating the protein kinase B signaling pathway". Oncology Letters 15, no. 3 (2018): 2939-2945. https://doi.org/10.3892/ol.2017.7699