An obligatory anaerobic Salmonella typhimurium strain redirects M2 macrophages to the M1 phenotype

  • Authors:
    • Mei Yang
    • Juan Xu
    • Qi Wang
    • An‑Qin Zhang
    • Kun Wang
  • View Affiliations

  • Published online on: January 8, 2018     https://doi.org/10.3892/ol.2018.7742
  • Pages: 3918-3922
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

A genetically engineered Salmonella typhimurium strain that may be applied in the medically useful therapeutic strategy of using bacterial agents to target breast cancer in a tumor‑bearing nude mouse model has been previously reported. Furthermore, immune cell accumulation in breast tumor types has been observed, particularly distributed in regions surrounding the bacteria. M2 macrophages are associated with breast cancer aggressiveness, whereas M1 macrophages are prone to devouring bacteria and killing cancer cells. Therefore, this engineered tumor‑targeting salmonella strain was used in an attempt to reverse the phenotype of M2 macrophages into the M1 phenotype. Subsequent to the co‑culture of M2 macrophages with the bacteria for a short time, >50% of the M2 macrophages were invaded by bacteria. These M2 macrophages exhibited a decreased expression of mannose receptor (an M2 phenotypic marker) and increased expression of human leukocyte antigen‑antigen D related (an M1 phenotypic marker). The results of the present study indicated that differentiated M2 macrophages may be redirected into the M1 phenotype following exposure to the engineered bacteria stimulus. This effect may be a potential mechanism by which bacteria retard tumor growth. Thus, this engineered bacterium may be a useful candidate for targeting and redirecting M2 macrophages into the M1 phenotype.
View Figures
View References

Related Articles

Journal Cover

March-2018
Volume 15 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Yang M, Xu J, Wang Q, Zhang AQ and Wang K: An obligatory anaerobic Salmonella typhimurium strain redirects M2 macrophages to the M1 phenotype. Oncol Lett 15: 3918-3922, 2018
APA
Yang, M., Xu, J., Wang, Q., Zhang, A., & Wang, K. (2018). An obligatory anaerobic Salmonella typhimurium strain redirects M2 macrophages to the M1 phenotype. Oncology Letters, 15, 3918-3922. https://doi.org/10.3892/ol.2018.7742
MLA
Yang, M., Xu, J., Wang, Q., Zhang, A., Wang, K."An obligatory anaerobic Salmonella typhimurium strain redirects M2 macrophages to the M1 phenotype". Oncology Letters 15.3 (2018): 3918-3922.
Chicago
Yang, M., Xu, J., Wang, Q., Zhang, A., Wang, K."An obligatory anaerobic Salmonella typhimurium strain redirects M2 macrophages to the M1 phenotype". Oncology Letters 15, no. 3 (2018): 3918-3922. https://doi.org/10.3892/ol.2018.7742