Dual role of CCL3/CCR1 in oral squamous cell carcinoma: Implications in tumor metastasis and local host defense

  • Authors:
    • Tarcilia Aparecida Silva
    • Fernanda Luiza Leite Ribeiro
    • Helenisa Helena De Oliveira-Neto
    • Satiro Watanabe
    • Rita De Cassia Gonçalves Alencar
    • Sandra Yasuyo Fukada
    • Fernando Queiroz Cunha
    • Claudio Rodrigues Leles
    • Elismauro Francisco Mendonça
    • Aline Carvalho Batista
  • View Affiliations

  • Published online on: November 1, 2007     https://doi.org/10.3892/or.18.5.1107
  • Pages: 1107-1113
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Chemokines are small chemotactic cytokines that can induce the migration of leukocytes, activate inflammatory/immune responses and have recently been implicated in the regulation of tumor growth and organ-specific spread. In this setting, the macrophage inflammatory protein-1α (CCL3) chemokine displays a diversity of roles that may contribute to the directional migration of squamous cells into cervical lymph nodes or to the defense against tumor initiation and progression. Thus, the aim of this study was to determine, for the first time, the expression of CCL3 and their receptors, CCR1 and CCR5, by real-time polymerase chain reaction in samples obtained from oral squamous cell carcinoma (OSCC) and healthy gingival tissue (control). In addition, we investigated the immunoexpression of these molecules in neoplastic cells (parenchyma), inflammatory/immune cells (stroma) in primary OSCC and in metastatic and non-metastatic lymph node tissues. The relationship of CCL3/CCR1 with survival data was also evaluated. The analysis of mRNA expression revealed a significantly higher expression of CCL3 and CCR1 in OSCC compared with the controls (P<0.05). The expression of CCR5 was not different in the two groups. The percentages of CCL3+ and CCR1+ cells were observed to be similar in parenchyma and stroma in the OSCC without lymph node metastasis when compared with OSCC with lymph node metastasis (P>0.05). However, we observed the density of CCL3+ nodal cells to be significantly higher in metastatic lymph nodes when compared with non-metastatic lymph nodes in the same patients (P<0.05). Considering CCL3 in stroma, the mean survival rate for patients with high CCL3+ cell percentage was better than for those with low CCL3+ cell percentage. Our findings suggest that the CCL3/CCR1 axis may have a role in the spread of tumoral cells to the lymph nodes and also in the local host defense against the tumor.

Related Articles

Journal Cover

November 2007
Volume 18 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Silva TA, Leite Ribeiro FL, De Oliveira-Neto HH, Watanabe S, De Cassia Gonçalves Alencar R, Fukada SY, Queiroz Cunha F, Rodrigues Leles C, Mendonça EF, Carvalho Batista A, Carvalho Batista A, et al: Dual role of CCL3/CCR1 in oral squamous cell carcinoma: Implications in tumor metastasis and local host defense. Oncol Rep 18: 1107-1113, 2007.
APA
Silva, T.A., Leite Ribeiro, F.L., De Oliveira-Neto, H.H., Watanabe, S., De Cassia Gonçalves Alencar, R., Fukada, S.Y. ... Carvalho Batista, A. (2007). Dual role of CCL3/CCR1 in oral squamous cell carcinoma: Implications in tumor metastasis and local host defense. Oncology Reports, 18, 1107-1113. https://doi.org/10.3892/or.18.5.1107
MLA
Silva, T. A., Leite Ribeiro, F. L., De Oliveira-Neto, H. H., Watanabe, S., De Cassia Gonçalves Alencar, R., Fukada, S. Y., Queiroz Cunha, F., Rodrigues Leles, C., Mendonça, E. F., Carvalho Batista, A."Dual role of CCL3/CCR1 in oral squamous cell carcinoma: Implications in tumor metastasis and local host defense". Oncology Reports 18.5 (2007): 1107-1113.
Chicago
Silva, T. A., Leite Ribeiro, F. L., De Oliveira-Neto, H. H., Watanabe, S., De Cassia Gonçalves Alencar, R., Fukada, S. Y., Queiroz Cunha, F., Rodrigues Leles, C., Mendonça, E. F., Carvalho Batista, A."Dual role of CCL3/CCR1 in oral squamous cell carcinoma: Implications in tumor metastasis and local host defense". Oncology Reports 18, no. 5 (2007): 1107-1113. https://doi.org/10.3892/or.18.5.1107