TLR3 dsRNA agonist inhibits growth and invasion of HepG2.2.15 HCC cells

  • Authors:
    • Li Chen
    • Yu-Yin Xu
    • Jia-Ming Zhou
    • Yuan-Yuan Wu
    • Qun E
    • Yuan-Yuan Zhu
  • View Affiliations

  • Published online on: April 30, 2012     https://doi.org/10.3892/or.2012.1791
  • Pages: 200-206
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Abstract

Toll-like receptor 3 (TLR3) is a pattern-recognizing receptor that is involved in immune signaling and plays a crucial role in survival by being able to recognize various viral components including double-stranded RNA (dsRNA). TLR3 expression and function in cancer cells are not well understood. In this study, we investigated whether TLR3 agonist dsRNA (BM-06) can inhibit proliferation and invasion, and promote apoptosis in HepG2.2.15 cells. HepG2.2.15 cells secreting hepatitis B virus (HBV) were treated with BM-06 and poly(I:C). Western blot analysis and PCR were employed to determine pharmacodynamic changes in biomarkers relevant to TLR3 signaling. Cell proliferation, invasion and apoptosis were analyzed by CCK-8 assay, transwell assay and flow cytometry. The expression of HBsAg, and HBcAg was observed by immunohistochemistry. Compared with untreated cells, pharmacological NF-κB activity of the TLR3 pathway by BM-06 (1.734-fold) or poly(I:C) (1.377-fold) was induced. By western blot analysis, we found that dsRNA induced TLR3-activated HepG2.2.15 cells which expressed NF-κB levels predominantly in the cytoplasmic fraction but fewer signals in the nucleus. BM-06 inhibited the proliferation, invasion and secretion of HBV, and induced apoptosis in HepG2.2.15 cells. In addition, the antitumor effects of BM-06 were superior to poly(I:C). Pharmacological activation of the TLR3 pathway by BM-06 can inhibit HepG2.2.15 cell growth.

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July 2012
Volume 28 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Chen L, Xu Y, Zhou J, Wu Y, E Q and Zhu Y: TLR3 dsRNA agonist inhibits growth and invasion of HepG2.2.15 HCC cells. Oncol Rep 28: 200-206, 2012.
APA
Chen, L., Xu, Y., Zhou, J., Wu, Y., E, Q., & Zhu, Y. (2012). TLR3 dsRNA agonist inhibits growth and invasion of HepG2.2.15 HCC cells. Oncology Reports, 28, 200-206. https://doi.org/10.3892/or.2012.1791
MLA
Chen, L., Xu, Y., Zhou, J., Wu, Y., E, Q., Zhu, Y."TLR3 dsRNA agonist inhibits growth and invasion of HepG2.2.15 HCC cells". Oncology Reports 28.1 (2012): 200-206.
Chicago
Chen, L., Xu, Y., Zhou, J., Wu, Y., E, Q., Zhu, Y."TLR3 dsRNA agonist inhibits growth and invasion of HepG2.2.15 HCC cells". Oncology Reports 28, no. 1 (2012): 200-206. https://doi.org/10.3892/or.2012.1791