Expression of glia maturation factor γ is associated with colorectal cancer metastasis and its downregulation suppresses colorectal cancer cell migration and invasion in vitro

  • Authors:
    • Huili Wang
    • Zhijiang Chen
    • Hongen Chang
    • Xiaoping Mu
    • Wenyu Deng
    • Zhaohu Yuan
    • Fang Yao
    • Yan Liu
    • Rongjia Mai
    • Bingyi Wu
  • View Affiliations

  • Published online on: January 9, 2017     https://doi.org/10.3892/or.2017.5361
  • Pages: 929-936
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Abstract

Glia maturation factor γ (GMFG) functions to reorganize the actin cytoskeleton and appears to play a causative role in cell migration and adherence. The present study assessed GMFG expression in colorectal cancer cells and tissue specimens and then explored the role of GMFG in colorectal cancer progression in vitro. GMFG protein was highly expressed in colorectal cancer tissues and a metastatic colon cancer cell line. Knockdown of GMFG expression using GMFG siRNA or anti-GMFG antibody decreased the capacity of colon cancer LoVo cell migration and invasion in vitro, while recombinant GMFG treatment induced LoVo cell migration. Furthermore, GMFG knockdown also decreased expression of MMP2 protein and reversed epithelial-mesenchymal transition (EMT) phenotypes in LoVo cells. Co-culture of LoVo cells with human umbilical vein endothelial cells (HUVECs) and exogenous GMFG treatment promoted LoVo cell migration and invasion. The data from the present study indicate that GMFG should be further evaluated as a biomarker for detection of colorectal cancer metastasis and that the targeting of GMFG expression or function could be a novel strategy in the future control of colorectal cancer.
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February-2017
Volume 37 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Wang H, Chen Z, Chang H, Mu X, Deng W, Yuan Z, Yao F, Liu Y, Mai R, Wu B, Wu B, et al: Expression of glia maturation factor γ is associated with colorectal cancer metastasis and its downregulation suppresses colorectal cancer cell migration and invasion in vitro. Oncol Rep 37: 929-936, 2017
APA
Wang, H., Chen, Z., Chang, H., Mu, X., Deng, W., Yuan, Z. ... Wu, B. (2017). Expression of glia maturation factor γ is associated with colorectal cancer metastasis and its downregulation suppresses colorectal cancer cell migration and invasion in vitro. Oncology Reports, 37, 929-936. https://doi.org/10.3892/or.2017.5361
MLA
Wang, H., Chen, Z., Chang, H., Mu, X., Deng, W., Yuan, Z., Yao, F., Liu, Y., Mai, R., Wu, B."Expression of glia maturation factor γ is associated with colorectal cancer metastasis and its downregulation suppresses colorectal cancer cell migration and invasion in vitro". Oncology Reports 37.2 (2017): 929-936.
Chicago
Wang, H., Chen, Z., Chang, H., Mu, X., Deng, W., Yuan, Z., Yao, F., Liu, Y., Mai, R., Wu, B."Expression of glia maturation factor γ is associated with colorectal cancer metastasis and its downregulation suppresses colorectal cancer cell migration and invasion in vitro". Oncology Reports 37, no. 2 (2017): 929-936. https://doi.org/10.3892/or.2017.5361