α-solanine enhances the chemosensitivity of esophageal cancer cells by inducing microRNA‑138 expression

  • Authors:
    • Jianbo Wu
    • Li Wang
    • Xinhui Du
    • Qianqian Sun
    • Yuanyuan Wang
    • Min Li
    • Wenqiao Zang
    • Kangdong Liu
    • Guoqiang Zhao
  • View Affiliations

  • Published online on: January 4, 2018     https://doi.org/10.3892/or.2018.6187
  • Pages: 1163-1172
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Abstract

Esophageal cancer is a common malignant tumor worldwide. Inherent and acquired drug resistance are the major challenges faced in anticancer chemotherapy. This study aimed to explore the effects of α-solanine in regards to the chemosensitivity of esophageal cancer cells. We found that α-solanine enhanced the sensitivity of EC9706 and KYSE30 cells to 5-flurouracil (5-FU) and cisplatin (Cis) by promoting drug-induced apoptosis. qRT-PCR and western blotting results showed that α-solanine treatment promoted miR-138 expression and decreased survivin expression in EC9706 and KYSE30 cells. α-solanine also enhanced the inhibitory effects of 5-Fu and Cis in EC9706 transplanted tumors in mouse models. Dual-Luciferase reporter assay results confirmed survivin as the direct target gene of miR-138. MiR-138 inhibited survivin expression in EC9706 and KYSE30 cells. And miR-138 mimic and si-survivin had similar effects with α-solanine in suppressing survivin expression and promoting cancer cell death. miR-138 inhibitor reversed the chemosensitivity-enhancing effect of α-solanine. In EC9706 and KYSE30 cells, survivin overexpression rescued the cancer cells from apoptosis caused by α-solanine and miR-138 mimic expression. From these findings, we conclude that α-solanine enhanced the chemosensitivity of esophageal cancer cells to chemotherapy via the miR-138/survivin pathway. This study provides insight into the molecular mechanism underlying the chemosensitivity-enhancing function of α-solanine and suggests a new chemotherapeutic strategy for esophageal cancer treatment.
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March-2018
Volume 39 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Wu J, Wang L, Du X, Sun Q, Wang Y, Li M, Zang W, Liu K and Zhao G: α-solanine enhances the chemosensitivity of esophageal cancer cells by inducing microRNA‑138 expression. Oncol Rep 39: 1163-1172, 2018
APA
Wu, J., Wang, L., Du, X., Sun, Q., Wang, Y., Li, M. ... Zhao, G. (2018). α-solanine enhances the chemosensitivity of esophageal cancer cells by inducing microRNA‑138 expression. Oncology Reports, 39, 1163-1172. https://doi.org/10.3892/or.2018.6187
MLA
Wu, J., Wang, L., Du, X., Sun, Q., Wang, Y., Li, M., Zang, W., Liu, K., Zhao, G."α-solanine enhances the chemosensitivity of esophageal cancer cells by inducing microRNA‑138 expression". Oncology Reports 39.3 (2018): 1163-1172.
Chicago
Wu, J., Wang, L., Du, X., Sun, Q., Wang, Y., Li, M., Zang, W., Liu, K., Zhao, G."α-solanine enhances the chemosensitivity of esophageal cancer cells by inducing microRNA‑138 expression". Oncology Reports 39, no. 3 (2018): 1163-1172. https://doi.org/10.3892/or.2018.6187