Peiminine serves as an adriamycin chemosensitizer in gastric cancer by modulating the EGFR/FAK pathway

  • Authors:
    • Qianqian Tang
    • Yunfei Wang
    • Lanjing Ma
    • Meiling Ding
    • Tingyu Li
    • Yongzhan Nie
    • Zhengyi Gu
  • View Affiliations

  • Published online on: January 3, 2018     https://doi.org/10.3892/or.2018.6184
  • Pages: 1299-1305
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Gastric cancer (GC) is one of the most common malignancies of the digestive tract. Adriamycin (ADR) has been widely utilized in various chemotherapy regimens for treating GC, yet its long-term application may increase drug resistance resulting in treatment failure. Increasing evidence shows that bioactive natural products can be used as chemotherapeutic sensitizers that can significantly improve chemotherapy sensitivity. Peiminine (PMI) is a biologically active component extracted from Fritillaria walujewii Regel. Thus, in the present study, we aimed to investigate whether peiminine (PMI) alters the chemosensitivity of GC to adriamycin (ADR). GC cells were treated with ADR with or without PMI. MTT assay, flow cytometry and a nude mouse tumor xenograft model of SGC7901 cells were used to evaluate the chemosensitization activity of PMI combined with ADR. Western blotting was used to examine the expression of cyclin D1 and cleaved PARP. The RayBio® Human RTK phosphorylation antibody array kit was used to test the differential protein expression. Compared with the ADR group, PMI combined with ADR significantly suppressed cell proliferation and induced cell apoptosis in vitro. The growth curve and tumor weight of the tumor xenografts were significantly decreased in mice treated with the combination of PMI and ADR. However, the organs showed no obvious abnormality after treatment with PMI plus ADR. The expression of cyclin D1 was decreased and the level of cleaved PARP was increased after treatment with PMI and ADR. The expression of p-EGFR and p-FAK was downregulated in cells treated with PMI and ADR, and the validation of p-EGFR and p-FAK was in accordance with the result of the phosphorylation antibody array kit. PMI may serve as a new chemosensitizer by inhibiting the proliferation and inducing the apoptosis to enhance the chemotherapeutic drug sensitivity of ADR in GC.
View Figures
View References

Related Articles

Journal Cover

March-2018
Volume 39 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Tang Q, Wang Y, Ma L, Ding M, Li T, Nie Y and Gu Z: Peiminine serves as an adriamycin chemosensitizer in gastric cancer by modulating the EGFR/FAK pathway. Oncol Rep 39: 1299-1305, 2018
APA
Tang, Q., Wang, Y., Ma, L., Ding, M., Li, T., Nie, Y., & Gu, Z. (2018). Peiminine serves as an adriamycin chemosensitizer in gastric cancer by modulating the EGFR/FAK pathway. Oncology Reports, 39, 1299-1305. https://doi.org/10.3892/or.2018.6184
MLA
Tang, Q., Wang, Y., Ma, L., Ding, M., Li, T., Nie, Y., Gu, Z."Peiminine serves as an adriamycin chemosensitizer in gastric cancer by modulating the EGFR/FAK pathway". Oncology Reports 39.3 (2018): 1299-1305.
Chicago
Tang, Q., Wang, Y., Ma, L., Ding, M., Li, T., Nie, Y., Gu, Z."Peiminine serves as an adriamycin chemosensitizer in gastric cancer by modulating the EGFR/FAK pathway". Oncology Reports 39, no. 3 (2018): 1299-1305. https://doi.org/10.3892/or.2018.6184