Emodin, an anthraquinones component of Rheum palmatun, has been used for anti-inflammatory purposes. However, its underlying molecular effect(s) on target cells remain to be well clarified. Thus, our current study was aimed at investigating the regulatory mechanism of emodin on liposaccharide-induced inflammatory responses in RAW 264.7 macrophages by RT-PCR, Western blot analysis, immunocytochemical staining and immunofluorescence analysis. It was found that a treatment of 20 µg/ml emodin inhibited the expression of a panel of inflammatory-associated genes, including TNFα, iNOS, IL-10, cytosolic IκBα, IKK-α and IKK-γ, to different extents as well as the nuclear translocation of NF-κB (nuclear factor-κB). The promoting effect of emodin on the production and translocation of p105 (the precursor of NF-κB p50) was time-dependent and reached a maximum at 5 h. Our data suggest that emodin plays its anti-inflammatory roles by regulating inflammatory cytokines, specifically by suppressing NF-κB activation.

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