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Effect of intermittent administration of teriparatide on the mechanical and histological changes in bone grafted with β‑tricalcium phosphate using a rabbit bone defect model
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- Published online on: November 1, 2017 https://doi.org/10.3892/etm.2017.5424
- Pages: 19-30
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Copyright: © Komatsu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Abstract
Introduction
In an aging society, hip, knee and shoulder replacement surgeries, as well as vertebrae fusion in spinal surgeries, are increasing in prevalence. Bone graft is essential, particularly for fusion in spinal surgery (1,2) and augmentation in revision arthroplasty (3–5), Bone grafting consists of filling the bone defect with a material to support new bone formation, and enhance healing and successful fusion of defects or nonunions (6,7).
Autograft, allograft and synthetic ceramics are used as materials for bone grafting. Iliac crest bone autograft is considered the gold standard for orthopedic surgery (6–8), as a sufficient amount of cancellous bone obtained from the pelvis exhibits all the desired properties of osteoconduction, osteoinduction and osteogenicity. Furthermore, the use of autologous bone has merits, including a lack of ethical issues, no concerns for disease transmission and no risk of immunogenicity. However, the autograft procedure requires an additional surgery to collect the bone for grafting, which may lead to complications such as paresthesia, long-lasting pain, hematoma and infection (9,10).
Allograft bone is harvested from tissue donors. Compared with the bone graft substitutes (synthetic ceramics) in use today, allograft bone is relatively inexpensive and readily available. Furthermore, it retains substantial structural strength and carries no associated donor site morbidity. However, the incorporation of allograft into the host bone is slower and less complete compared with autograft (11). In addition, there is a theoretical risk of disease transmission and immune rejection (10).
Synthetic bone graft substitutes are osteoconductive agents that consist of hydroxyapatite and β-tricalcium phosphate (β-TCP), or a combination of these materials (12,13). Hydroxyapatite is a naturally occurring mineral form of calcium apatite with the formula Ca5(PO4)3(OH), and possesses the properties of biocompatibility and osteoconductivity (14,15). However, it has various disadvantages, such as remaining in the body for a long time and showing no progressive bone formation in the course of bone tissue repair (16). By contrast, β-TCP is a synthetic porous ceramic graft material composed of tricalcium phosphate [TCP; Ca3(PO4)2,], which comprises 70% of human bone (17–21). During the bone remodeling process, β-TCP is gradually degraded by osteoclastic resorption and finally replaced with mature host bone (22,23). Thus, β-TCP is a highly biocompatible material that provides a resorbable interlocking network within a bone defect (24). However, β-TCP must be protected from excessive loading forces until a solid fusion has taken place, due to its brittle structure and low tensile strength (23,25–27).
Parathyroid hormone (PTH) is a peptide hormone consisting of 84 amino acids that is involved in calcium homeostasis; its secretion from the parathyroid gland is mainly controlled by serum Ca2+ through negative feedback (28). Teriparatide is a biologically active fragment containing the N-terminal 34 amino acids of human parathyroid hormone (hPTH), and daily and weekly teriparatide administration has been approved for the treatment of osteoporosis (29,30). When teriparatide is administered intermittently, it has an anabolic effect on osteoblasts, thereby stimulating bone formation and increasing bone mineral density (31,32). In 1999, Andreassen et al (33) reported the efficacy of intermittent administration of teriparatide in a rat tibial fracture model. Teriparatide was demonstrated to promote bone formation by increasing the number and activity of osteoblasts, enhancing the mean cortical thickness and trabecula volume, and improving bone microarchitecture, thereby increasing fracture strength and callus quantity. Similarly, intermittent administration of teriparatide has previously been demonstrated to increase the volume, stiffness, torsional strength and density of fracture calluses in a rat femur fracture model (34,35). These observations suggest that teriparatide enhances and accelerates not only osteogenesis but also bone remodeling in the process of bone repair (36,37).
The aim of the present study was to investigate whether intermittent administration of teriparatide with low frequency (three times per week) enhances the remodeling of bone defects grafted with β-TCP, using a rabbit bone defect model, based on radiographic and histological examinations, and mechanical testing.
Materials and methods
Preparation of β-TCP
In the present study, two types of β-TCP granules with a total porosity of 67% (KG-2) and 75% (KG-3) were supplied by HOYA Corporation (Tokyo, Japan). β-TCP granules contain three types of pores: Macropores (diameter, 100–300 µm), interconnected pores (diameter, 50–100 µm) and micropores (diameter, 0.5–10 µm). β-TCP slurry was produced by HOYA Corporation by mixing dibasic calcium phosphate and calcium carbonate, and milled with water (quantities not known); the slurry was subsequently sintered and dried to produce β-TCP powder, which was burnt at 1,100°C with surfactant and bubbled stabilizer (types not known) to produce β-TCP granules containing three types of pores (macropore, interconnected pore and micropore). The compressive strength is 15 MPa for 67% porosity and 1.5 MPa for 75% porosity.
Teriparatide
Teriparatide, also known as hPTH (1–34), corresponds to the N-terminal part of hPTH, the full length of which is 84 amino acids (38). Teriparatide acetate was supplied by Asahi Kasei Pharma Corp. (Tokyo, Japan), dissolved in 0.2% rabbit serum albumin solution (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany), and stored at −30°C until use.
Bone defect model
The present study was approved by the Ethics Committee of Juntendo University Institute of Casualty Center (Juntendo University Shizuoka Hospital, Izunokuni, Japan). A total of 60 male Japanese white rabbits (12 weeks old; 3.5 kg; Japan SLC, Inc., Hamamatsu, Japan) were divided into 10 groups: Sham group for 4 weeks; 67% β-TCP without teriparatide for 4 weeks; 67% β-TCP with teriparatide for 4 weeks; 75% β-TCP without teriparatide for 4 weeks; 75% β-TCP with teriparatide for 4 weeks; Sham group for 8 weeks; 67% β-TCP without teriparatide for 8 weeks; 67% β-TCP with teriparatide for 8 weeks; 75% β-TCP without teriparatide for 8 weeks; and 75% β-TCP with teriparatide for 8 weeks. The rabbits were housed separately in standard cages in a temperature-controlled room (24±3°C and humidity of 55±15%) with a 12-h light/dark cycle, fed a commercial standard diet (NR-2; Nisseiken Co. Ltd., Tokyo, Japan) and received drinking water ad libitum.
Rabbits were anesthetized with an intravenous bolus injection of 25 mg/kg sodium pentobarbital (Somnopentyl; Kyoritsu Seiyaku Corporation, Tokyo, Japan) prior to surgery. The distal metaphysis and lateral condyle of the femur were exposed through a 2-cm lateral longitudinal incision, and the thigh muscles were divided under sterile conditions. The bone membrane was removed and a dead-end defect (5 mm in diameter and 15 mm in depth) was created in the lateral cortex just proximal to the epiphyseal plate using an air drill with an intermittent drip of sterile saline to control the temperature. The orientation of the bone defect was perpendicular to the sagittal axis of the femur.
β-TCP granules (70 mg of those with 75% porosity or 80 mg of those with 67% porosity) respectively, were manually grafted into the defected hole (5 mm in diameter and 15 mm in depth corresponding to a volume of 294 mm3). The soft tissue was closed in layers. All procedures were performed by the same surgeon. Following the completion of surgery, teriparatide (40 µg/kg), or 0.2% rabbit serum albumin solution as a vehicle control, was subcutaneously administered to rabbits in the PTH and Control groups of animals, respectively, three times per week. In some experiments, the bone defect was created in the distal metaphysis and lateral condyle of the femur and no β-TCP granules were grafted. These animals were subcutaneously injected with 0.2% rabbit serum albumin solution three times per week and used as the Sham group.
At 4 or 8 weeks post-surgery, rabbits were anesthetized via the intraperitoneal injection of 50 mg/kg pentobarbital sodium. Rabbits were sacrificed by exsanguination via the femoral vein under deep anesthesia. Death of the animals was confirmed by cardiac arrest and cessation of respiration. Blood samples were harvested from the femoral vein, and the serum was separated by centrifugation at 1,220 × g for 20 min at 4°C and stored at −80°C. Following sacrifice, femurs was collected from every rabbit.
In order to evaluate bone formation and remodeling in vivo, all rabbits were subcutaneously injected twice with calcein, a calcium-binding fluorescent dye (10 mg/kg; Wako Pure Chemical Industries, Ltd., Osaka, Japan) on days 3 and 10 prior to sacrifice.
Assay of Gla-osteocalcin
Serum levels of Gla-osteocalcin (Gla-OC) were measured using an ELISA kit (Gla-OC EIA kit; Takara Bio, Inc., Shiga, Japan) as previously described (38,39).
Radiological analysis & measurement of bone mineral density (BMD)
Distal femoral condyles containing graft sites were dissected from the femurs, and X-ray images were captured using micro computerized tomography (CT) with a SkyScan 1172 instrument (Bruker microCT, Konitich, Belgium) to evaluate the absorption of β-TCP and new bone formation. BMD values at the femoral condyle graft sites were calculated from micro-CT images using Image Pro (version 7; Media Cybernetics Inc., Rockville, MD, USA) with reference to BMD phantom.
Histological analyses
Distal femoral condyles containing the graft sites were fixed in 4% formaldehyde in 0.1 M phosphate buffer (pH 7.2) at 4°C for 20 h and subsequently dehydrated with 70% alcohol, embedded in 2-hydroxyethyl methacrylate/methyl methacrylate/2-hydroxyethyl acrylate mixed resin, and cut into 3-µm sections, as previously reported (40–42). These sections were stained with Giemsa. Alternatively, the sections were histochemically stained for tartrate resistant acid phosphatase (TRAP) activity and counterstained with hematoxylin and nuclear fast red (43). Histomorphometric analyses were performed using BIOREVO (Keyence, Osaka, Japan), and Image Pro (version 7), and the graft sites of femoral condyles were analyzed in a region (0.55×2.2 mm), which was centrally aligned in a created bone defect. The quantitative histomorphometric analysis of trabecula remodeling was performed according to the following stereologic calculations (44); i) bone volume (BV)/tissue volume (TV; %); ii) mineralized surface (MS)/bone surface[BS; %; (double labeled surface+single labeled surface/2)/BS]; iii) mineral apposition rate (MAR; µm/day; interlabel thickness/interlabel time); iv) bone formation rate (BFR)/BS (µm3/µm2/year; MAR × MS/BS); v) osteoclast number (N.Oc)/BS (N/mm); vi) osteoclast surface (Oc.S)/BS (%); and vii) osteoid surface (OS)/BS (%). Furthermore, the change in β-TCP volume in the graft was evaluated using the following parameters; viii) β-TCP volume (TCPV)/TV (%); and ix) newly formed bone volume (BV-TCPV)/TV (%).
MAR was measured by calculating calcein-labeling under a fluorescence microscope with excitation and emission wavelengths of 495 and 515 nm, respectively. BV/TV, MS/BS, MAR, BFR/BS, TCPV/TV and (BV-TCPV)/TV were calculated using Giemsa-stained sections. An osteoclast was defined as a multinucleated giant cell in contact with the surface of a bone or bone substitute, and N.Oc/BS and Oc.S/BS were calculated using TRAP-stained sections.
In the Control group, histomorphometry was performed using sections of distal femoral condyles without bone defect obtained from unoperated femurs.
Mechanical testing
The grafts were evaluated with an axial push-out load to failure test using MTS858 Mini Bionix2 (MTS Systems Corporation, Eden Prairie, MN, USA) and the appropriate software (MTS Test Star 790.00 version 4.00; MTS Systems Corporation). The specimens were placed on a metal piston jig with a diameter of 4.0 mm, and continuous load-displacement data at a test speed of 0.5 mm/min were recorded until the graft surface was depressed to a depth of 2.0 mm. The maximum shear stiffness (Mpa/mm) was obtained from the slope of the linear section of the load-displacement curve, and the total energy absorption (J/m2) was calculated as the area under the load-displacement curve. Maximum shear strength (MPa) was determined from the maximum force applied until failure of the bone and artificial bone (45).
Statistical analysis
All data are expressed as the mean ± standard deviation. Statistical analysis was performed using one-way analysis of variance followed by Bonferroni's multiple comparison test (GraphPad Prism; GraphPad Software, Inc., La Jolla, CA, USA). P<0.05 was considered to indicate a statistically significant difference.
Results
Evaluation of bone metabolism using Gla-OC
Following grafting with β-TCP, teriparatide (40 µg/kg) or a vehicle was subcutaneously injected into rabbits for 4 or 8 weeks, and serum Gla-OC, which is a specific marker for bone formation, was subsequently measured. The Gla-OC levels were significantly higher in the PTH group than in the Sham (P<0.01) and Control groups (P<0.05) at both 4 and 8 weeks (Fig. 1). By contrast, no significant difference in Gla-OC levels was observed between the Control (with β-TCP graft) and Sham (without β-TCP graft) groups at 4 or 8 weeks. These observations suggest that increased levels of serum Gla-OC may be due to teriparatide-induced bone formation but not the β-TCP graft.
Radiological analysis & measurement of BMD
Micro-CT analysis revealed that β-TCP was clearly present in the grafted bone in the Control group at 4 weeks following graft surgery; however, β-TCP was absorbed and the granular structure was markedly increased in the grafted bone around β-TCP granules in the PTH group for both porosities of β-TCP (Fig. 2A).
Furthermore, micro-CT analysis indicated that, at 8 weeks, β-TCP was mostly absorbed and replaced with trabecular structure, possibly cancellous bone, in the Control group (Fig. 2A), and the structure was notably increased in the grafted bones with both the porosity of 67 and 75% in the PTH group (Fig. 2A).
BMD was quantified based on the results of micro-CT analysis. The results indicated that BMD was significantly decreased (~50%) between 4 and 8 weeks following the graft with the porosity of 67 (P<0.001) and 75% (P<0.01; Fig 2B) in the Control group. By contrast, BMD was only decreased by ~30% in the PTH group following the graft with 67% porosity, although this reduction was significant (P<0.001; Fig. 2B). The reduction in BMD was almost completely suppressed in the PTH group following the graft with 75% porosity, with no significant differences in BMD observed between weeks 4 and 8 (Fig. 2C). Notably, BMD was significantly increased in the PTH group compared with the Control group at 8 weeks post-graft for both 67 and 75% porosity (P<0.01; Fig. 2B and C). These observations suggest that teriparatide administration enhances calcification in bone defects grafted with β-TCP of 67 and 75% porosity.
Histological analysis
Histological analysis revealed that the number of TRAP-positive osteoclasts was markedly increased in the PTH group compared with the Control group for bone grafted with β-TCP at porosities of 67 and 75% (Fig. 3). TRAP-positive osteoclasts were in direct contact with the surface of newly formed bone as well as β-TCP, with a ragged appearance of these structures. Furthermore, the amount of β-TCP at porosities of 67 and 75% was markedly decreased at 8 weeks compared with 4 weeks in both the Control and PTH groups (Fig. 4). Notably, the amount of newly formed bone was increased in the PTH group compared with the Control group at 4 and 8 weeks following the graft for both porosities of β-TCP (Fig. 4).
Histomorphometry
Histomorphometric analysis was first performed using sections of distal femoral condyles without bone defects, obtained from unoperated femurs. The results demonstrated a significant increase in BV/TV (P<0.05) and BFR/BS (P<0.01) at 4 weeks (Fig. 5A and B), and significant increases in BV/TV, MAR and BFR/BV at 8 weeks (P<0.01; Fig. 5A-C) in the PTH group compared with the Control group (without teriparatide). These observations suggest that teriparatide administration enhances the BV, MAR and BFR in distal femoral condyles without bone defect. No significant differences were observed in Oc.S/BS or OS/BS between the Control and PTH groups at weeks 4 or 8 (Fig. 5D and E).
Furthermore, histomorphometric analysis was performed using sections of distal femoral condyles with bone defects and β-TCP grafts (Figs. 6 and 7). The volume of β-TCP (TCPV/TV) was decreased between weeks 4 and 8 in both the Control and PTH groups following grafts with 67 and 75% porosity (Figs. 6A and 7A, respectively). By contrast, the volume of newly formed bone, (BV- TCPV)/TV, was significantly increased between weeks 4 to 8 in the PTH groups with grafts of both porosities (P<0.05; Figs. 6B and 7B). Additionally, the volume of newly formed bone was significantly increased in the PTH group compared with the Control group grafted with 67% β-TCP at 4 weeks (P<0.05; Fig. 6B).
The calcification rate (MAR) was significantly higher in the PTH group compared with the Control group at 4 and 8 weeks following grafting with both porosities of β-TCP (P<0.05 for 67%, P<0.01 for 75%; Figs. 6C and 7C, respectively). Calcification was indicated to be increased in the PTH group compared with the Control group at 4 and 8 weeks following grafting with both porosities of 67 and 75% β-TCP by labeling with a calcium-binding fluorescent dye calcein (Fig. 8). BFR/BS was significantly increased in the PTH group compared with the Control group at 4 weeks for both porosities of β-TCP (P<0.05); however, no significant differences were observed at 8 weeks after bone grafting (Figs. 6D and 7D).
N.Oc/BS and Oc.S/BS were significantly increased in the PTH group compared with the Control group for both porosities of β-TCP at 4 weeks (P<0.05) and 8 weeks (P<0.01) following the bone graft (Fig. 6E and F, and Fig. 7E and F). Furthermore, OS/BS significantly increased in the Control group from week 4 to week 8 for the two porosities of β-TCP (P<0.05); however, OS/BS was significantly lower in the PTH group compared with the Control group with β-TCP porosities of 67 (P<0.01) and 75% (P<0.001) at 8 weeks following the bone graft (Figs. 6G and 7G). This reduction in OS in the PTH group may be due to the action of teriparatide, which substantially enhances the bone formation, thereby decreasing OS/BS.
Mechanical strength analysis
Maximum shear strength of the bone grafts was significantly increased in the PTH groups compared with the Control groups at 4 weeks for β-TCP grafts of 67% porosity (P<0.05; Fig. 9A) and at 8 weeks for β-TCP grafts of both porosities (P<0.05 for 67%, P<0.01 for 75%; Fig. 9A and B). Notably, the maximum shear strength was significantly decreased between 4 and 8 weeks in the Control groups at both porosities of β-TCP (P<0.001; Fig. 9A and B); however, the maximum shear strength was maintained by teriparatide administration to almost the same levels at 8 weeks as those of the Control groups at 4 weeks for both porosities of β-TCP (Fig. 9A and B).
A significant increase in maximum shear stiffness was observed in the PTH group compared with the control at 4 (P<0.05) and 8 (P<0.01) weeks following the application of bone grafts with 67% porosity β-TCP (Fig. 9C); however, no significant difference in this parameter was observed between groups for the 75% porosity graft (Fig. 9D). Significant increases in total energy absorption were also observed in the PTH group compared with the control for the 67% porosity graft (P<0.001 at week 4 and P<0.05 at week 8; Fig. 9E), but not for the graft using 75% β-TCP (Fig. 9F).
Discussion
Grafting β-TCP is a well-established method for restoring bone defects; however, there is a concern that the mechanical stability of grafted β-TCP is not maintained during bone translation (23). Notably, the intermittent administration of teriparatide (hPTH 1–34) reduces the risk of fracture in patients with osteoporosis and works as an anabolic agent to stimulate bone formation (46). In the present study, therefore, the effect of intermittent administration of teriparatide on new bone formation and mechanical strength of bone defects grafted with β-TCP was investigated. The results indicated that intermittent teriparatide administration suppressed the reduction in mechanical strength during the remodeling process in bone defects grafted with β-TCP. Furthermore, the results of the present study indicate that teriparatide increases the degradation of β-TCP by osteoclastic resorption and promotes the formation of new bone following grafting. These observations demonstrate that teriparatide is effective at maintaining the mechanical stability of grafted β-TCP, possibly by promoting new bone formation.
The experimental model used in the present study was designed to evaluate the effect of intermittent administration of teriparatide on the regeneration of cancellous bone defects grafted with β-TCP. White Japanese rabbits were chosen as a testing model due to the ease of handling and the anatomical characteristics of their femurs (47,48), and bone metabolism closely resembling that in humans (49–53). Previous studies have indicated that the femoral condyle may be used as a site for evaluating the mechanical strength and bone formation in defects grafted with bone substitutes (47,48,54–56). In the present study, the radiological, histological and mechanical strength analyses of defected bone were performed at 4 and 8 weeks following β-TCP graft; these intervals were selected because a period of 6 weeks is required for bone formation in rabbits (27,57–59), and teriparatide accelerates the bone remodeling process 4 weeks following intermittent administration (31,60,61).
The anabolic effect of teriparatide is dependent on the dose, frequency and duration of administration (62–64). In previous studies, 6–10 µg/kg teriparatide was subcutaneously injected into rats and rabbits three times per week (62–64). In the present study, to make the effect of teriparatide more evident, 40 µg/kg teriparatide was subcutaneously injected three times per week, and it was confirmed that teriparatide significantly increased the serum levels of Gla-OC at 4 and 8 weeks. Furthermore, teriparatide significantly increased the calcification, bone formation and newly formed bone following the graft compared with that in the Control group. These results indicate that teriparatide enhances bone formation in bone defects grafted with β-TCP. Micro-CT analysis also revealed that the reduction in BMD during the experimental period was suppressed, and BMD was maintained during the experimental period in the teriparatide-treated group following the graft.
In the Control group, TCPV was significantly decreased between weeks 4 and 8, whereas the volume of newly formed bone was increased. However, maximum shear strength significantly decreased between weeks 4 and 8 in the Control group. These results suggest that the bioresorption of β-TCP causes the mechanical loss of bone grafted with β-TCP, although the volume of newly formed bone increases, as previously reported (21,23,65). Notably, teriparatide further decreased the volume of β-TCP and increased the volume of newly formed bone between weeks 4 and 8 compared with the Control group at both porosities of β-TCP. These results suggest that teriparatide increases both the biodegradation of β-TCP and new bone formation in bone defects grafted with β-TCP. In this context, it is interesting to note that based on the activity of teriparatide (inducing osteoclast differentiation and activation), bone resorption is transiently increased at an early stage following teriparatide administration; however, bone formation is significantly increased at a late phase following the administration (66). Importantly, teriparatide administration increased all mechanical parameters (maximum shear strength, maximum shear stiffness and total energy absorption) at 4 and 8 weeks compared with the Control group, when β-TCP with 67% porosity was used. By contrast, only maximum shear strength was increased by teriparatide at 8 weeks when using the porosity of 75%. These results suggest that β-TCP of 67 but not 75% porosity may be useful as a bone substitute, which enhances the remodeling and mechanical strength of bone defects, potentially by promoting the resorption of β-TCP and new bone formation.
It has been reported that during the bone remodeling process in β-TCP grafted sites, osteoclasts continuously adhere to the surface of β-TCP and resorb the material, and their biodegradation stimulates bone formation (67–70). Furthermore, a previous study indicated that the number of osteoclasts peaks early, whereas the rate of new bone formation peaks later in bone defects grafted with β-TCP (27). Based on these observations, it may be speculated that the bioresorption of β-TCP by osteoclasts is responsible for the reduction in mechanical strength of bone grafted with β-TCP until new bone formation is completed. Consistent with this, the maximum shear strength was reduced between weeks 4 and 8 in the Control group. Notably, the maximum shear strength was maintained by teriparatide administration, possibly via the increase in calcification or new bone formation.
In conclusion, the results of the present study revealed that intermittent administration of teriparatide enhances the remodeling of bone defects grafted with β-TCP. Teriparatide likely increases the degradation of β-TCP by osteoclastic resorption and promotes the formation of new bone following grafting, thereby suppressing the reduction in mechanical strength during the remodeling process of bone defects grafted with β-TCP. Thus, the combination of an anabolic agent (e.g., teriparatide) and a bone graft substitute (e.g., β-TCP) may have useful clinical applications in spinal fusion surgery and augmentation in revision arthroplasty.
Acknowledgements
The authors would like to thank Associate Professor Kiyohito Naito (Department of Medicine for Motor Organs, Juntendo University Graduate School of Medicine, Tokyo, Japan) for helpful discussion, Masahiro Miyazaki (Department of Bio-Engineering, Juntendo University Institute of Casualty Center, Shizuoka, Japan) and the members of the Laboratory of Division of Molecular & Biochemical Research, Research Support Center, Juntendo University Graduate Faculty of Medicine (Tokyo, Japan), for technical assistance. The authors would also like to thank Asahi Kasei Pharma Corp. and HOYA Corporation for their supplies of teriparatide and β-TCP, respectively. This study was supported in part by a grant from the Strategic Research Foundation Grant-aided Project for Private Universities from Ministry of Education, Culture, Sport, Science, and Technology, Japan, 2014–2018 (grant no. S1411007).
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