Biological phenotype determination with ex vivo model in gastric cancer for matrix-metalloproteinase inhibitor treatment

Rha,Sun Young; Jeung,Hei Cheul; Roh,Jae Kyung; Kim,Jin Ju; Noh,Sung Hoon; Min,Jin Sik; Kim,Byung Soo; Chung,Hyun Cheol

doi:10.3892/ijmm.10.3.251

September 2002 Volume 10 Issue 3

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September 2002 Volume 10 Issue 3

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Article

Biological phenotype determination with ex vivo model in gastric cancer for matrix-metalloproteinase inhibitor treatment

Authors:
- Sun Young Rha
- Hei Cheul Jeung
- Jae Kyung Roh
- Jin Ju Kim
- Sung Hoon Noh
- Jin Sik Min
- Byung Soo Kim
- Hyun Cheol Chung
View Affiliations / Copyright

Affiliations: Cancer Metastasis Research Center, Yonsei University College of Medicine, Seoul 120-752, Korea;
Pages: 251-256
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Published online on: September 1, 2002

https://doi.org/10.3892/ijmm.10.3.251
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Abstract

Among the many biological characteristics of cancer, matrix-metalloproteinases (MMPs) are essential for tumor invasion and metastasis. To test the possibility of ex vivo model as a therapeutic guideline for MMP inhibitor (MMPI) treatment, we evaluated IC50 of the gabexate mesylate against MMP-9. Thirty-four paired normal and gastric cancer tissues were tested to measure the IC50 of the gabexate mesylate. MMP-9 activity was measured by zymography. Both MMP-9 expression (p=0.04) and IC50 (p=0.02) were higher in cancer than normal tissues. IC50 of the cancer tissues was higher than paired normal tissues especially in cases with large tumor (≥5 cm) (p=0.03), higher T-stage (p=0.04), lymph node metastasis (p=0.04) and advanced stage (p=0.04). In cancers extending beyond submucosa or in diffuse/mixed type, a tendency of higher IC50 was observed than tumors confined to submucosa or intestinal type cancer despite similar MMP-9 activity between the groups. Patients with high IC50 showed poorer prognosis than patients with low IC50 in curatively-resected group. In multivariate analysis, high IC50 was suggested as an independent prognostic factor. We were able to differentiate the high risk patients using IC50 of gabexate mesylate in ex vivo model. This model can be applied in detecting patients with poor prognosis and patients who may benefit from MMPI treatment.

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Biological phenotype determination with ex vivo model in gastric cancer for matrix-metalloproteinase inhibitor treatment

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Abstract

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