Up-regulation of WNT8B mRNA in human gastric cancer

  • Authors:
    • Tetsuroh Saitoh
    • Tetsuya Mine
    • Masaru Katoh
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  • Published online on: February 1, 2002     https://doi.org/10.3892/ijo.20.2.343
  • Pages: 343-348
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Abstract

WNT - β-catenin - TCF signaling pathway is activated by Xenopus wnt-8 (Xwnt-8) during Xenopus early development, and dysregulated activation of β-catenin - TCF signaling pathway in mammalian cells leads to carcinogenesis. We have previously cloned and characterized human WNT8A, one of human orthologues of Xwnt-8. Here, we cloned and characterized human WNT8B by using bioinformatics, cDNA-PCR, and RACE. WNT8B gene of about 23-kb in size consisted of six exons, and encoded a 351-amino-acid polypeptide with the N-terminal signal peptide and two N-linked glycosylation sites. C-terminal region of WNT8B, WNT8A, WNT2, and WNT2B were longer than that of other human WNTs. Thirty-five nucleotide changes between WNT8B isolated by us and WNT8B isolated by another group resulted in Gly230Ala and Arg284Leu amino-acid substitutions. Gly230 and Arg284 of WNT8B were conserved in WNT8A. Gly230-Arg284 WNT8B allele was also identified in human genome draft sequences AL133352.10, AL359759.18, and human EST BF732616. These results indicate that the Gly230-Arg284 WNT8B cDNA isolated in this study is derived from the more common WNT8B allele. WNT8B mRNAs of 4.4- and 3.5-kb in size were weakly detected in a colorectal cancer cell line SW480, but were undetectable in any normal human tissues by using Northern blot analyses. WNT8B was significantly up-regulated in gastric cancer cell lines KATO-III (signet-ring cell carcinoma) and MKN45 (poorly differentiated adenocarcinoma), and also in 5 out of 10 cases of primary gastric cancer. WNT8B might play key roles in gastric cancer through activation of the β-catenin - TCF signaling pathway.

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February 2002
Volume 20 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Saitoh T, Mine T and Katoh M: Up-regulation of WNT8B mRNA in human gastric cancer. Int J Oncol 20: 343-348, 2002.
APA
Saitoh, T., Mine, T., & Katoh, M. (2002). Up-regulation of WNT8B mRNA in human gastric cancer. International Journal of Oncology, 20, 343-348. https://doi.org/10.3892/ijo.20.2.343
MLA
Saitoh, T., Mine, T., Katoh, M."Up-regulation of WNT8B mRNA in human gastric cancer". International Journal of Oncology 20.2 (2002): 343-348.
Chicago
Saitoh, T., Mine, T., Katoh, M."Up-regulation of WNT8B mRNA in human gastric cancer". International Journal of Oncology 20, no. 2 (2002): 343-348. https://doi.org/10.3892/ijo.20.2.343