A study of 5-aminolevulinic acid and its methyl ester used in in vitro and in vivo systems of human bladder cancer

  • Authors:
    • Vanaja Manivasager
    • Paul Wan Sia Heng
    • Jinsong Hao
    • Wei Zheng
    • Khee Chee Soo
    • Malini Olivo
  • View Affiliations

  • Published online on: February 1, 2003     https://doi.org/10.3892/ijo.22.2.313
  • Pages: 313-318
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Abstract

The use of 5-aminolevulinic acid and its esters to induce endogenous porphyrins for the purpose of detection of epithelial cancers is being studied extensively in many centres around the world. The challenge is to prepare an efficacious formulation for the purpose of cancer detection. Photodynamic diagnosis of cancer using 5-aminolevulinic acid (ALA) and its ester derivatives is being actively investigated. In this study, we compared ALA with ALA methyl ester (AME) derivative in terms of PpIX fluorescence intensity in in vitro and in vivo systems of bladder carcinoma. For the in vivo system consisting of RT112 xenografts, the modes of drug administration compared were intravenous administration and topical application. The Karl Storz fluorescence endoscopy system was used to obtain macroscopic fluorescence images. The macroscopic images were further analysed for fluorescence intensity distribution. For the intravenous administration, over all time points studied (1, 3, 6 h), AME-PpIX fluorescence was lower than ALA-PpIX fluorescence and was cleared at a faster rate than the ALA-PpIX when administered intravenously. Topical application with two different polymers, Gantrez and Polyvinyl pyrrolidone (PVP) which are fast releasing polymers was found to be comparable in inducing PpIX fluorescence. Topical AME-PpIX fluorescence was found to be comparable with ALA-PpIX fluorescence. The results of this study suggest that the AME can also be used as a good diagnostic agent.

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February 2003
Volume 22 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Manivasager V, Heng PW, Hao J, Zheng W, Soo KC and Olivo M: A study of 5-aminolevulinic acid and its methyl ester used in in vitro and in vivo systems of human bladder cancer. Int J Oncol 22: 313-318, 2003.
APA
Manivasager, V., Heng, P.W., Hao, J., Zheng, W., Soo, K.C., & Olivo, M. (2003). A study of 5-aminolevulinic acid and its methyl ester used in in vitro and in vivo systems of human bladder cancer. International Journal of Oncology, 22, 313-318. https://doi.org/10.3892/ijo.22.2.313
MLA
Manivasager, V., Heng, P. W., Hao, J., Zheng, W., Soo, K. C., Olivo, M."A study of 5-aminolevulinic acid and its methyl ester used in in vitro and in vivo systems of human bladder cancer". International Journal of Oncology 22.2 (2003): 313-318.
Chicago
Manivasager, V., Heng, P. W., Hao, J., Zheng, W., Soo, K. C., Olivo, M."A study of 5-aminolevulinic acid and its methyl ester used in in vitro and in vivo systems of human bladder cancer". International Journal of Oncology 22, no. 2 (2003): 313-318. https://doi.org/10.3892/ijo.22.2.313