Type I interferon prolongs cell cycle progression via p21WAF1/CIP1 induction in human colon cancer cells

  • Authors:
    • Tomonari Katayama
    • Kazuaki Nakanishi
    • Hiroshi Nishihara
    • Naoya Kamiyama
    • Takahito Nakagawa
    • Toshiya Kamiyama
    • Ken Iseki
    • Shinya Tanaka
    • Satoru Todo
  • View Affiliations

  • Published online on: September 1, 2007     https://doi.org/10.3892/ijo.31.3.613
  • Pages: 613-620
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Abstract

Type I interferon (IFN) was originally identified as an immunomodulatory cytokine because of its antiviral activity. Further characterization of its biological effects revealed a prominent role in the direct control of cell growth and potent immunomodulatory and antiangiogenic actions. IFN-α and IFN-β had both been classified as type I IFN, but differences in their antitumor activities were reported. We confirmed the difference in the antiproliferative activities of IFN-α2b and IFN-β toward HT29 and SW480 cells. IFN treatment was observed to prolong cell cycle progression; in particular, the accumulation of S-phase population was one of the most characteristic changes. The prolongation of S-phase progression and transition into G2/M-phase was suggested to be a crucial action of type I IFN on colon cancer. Additionally, IFN activated the p21 promoter gene and induced p21WAF1/CIP1 expression. Furthermore, the cell cycle prolongation effect of IFN was suppressed when p21 expression was downregulated. Therefore, we confirmed that p21WAF1/CIP1 was a crucial target molecule for the effects of IFN on the cell cycle. Additionally, the ability of p21 induction differed between IFN-α2b and IFN-β and correlated with their inhibitory activities toward cell growth. We conclude that type I IFN prolongs cell cycle progression by p21WAF1/CIP1 induction in human colon cancer cells.

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September 2007
Volume 31 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Katayama T, Nakanishi K, Nishihara H, Kamiyama N, Nakagawa T, Kamiyama T, Iseki K, Tanaka S and Todo S: Type I interferon prolongs cell cycle progression via p21WAF1/CIP1 induction in human colon cancer cells. Int J Oncol 31: 613-620, 2007.
APA
Katayama, T., Nakanishi, K., Nishihara, H., Kamiyama, N., Nakagawa, T., Kamiyama, T. ... Todo, S. (2007). Type I interferon prolongs cell cycle progression via p21WAF1/CIP1 induction in human colon cancer cells. International Journal of Oncology, 31, 613-620. https://doi.org/10.3892/ijo.31.3.613
MLA
Katayama, T., Nakanishi, K., Nishihara, H., Kamiyama, N., Nakagawa, T., Kamiyama, T., Iseki, K., Tanaka, S., Todo, S."Type I interferon prolongs cell cycle progression via p21WAF1/CIP1 induction in human colon cancer cells". International Journal of Oncology 31.3 (2007): 613-620.
Chicago
Katayama, T., Nakanishi, K., Nishihara, H., Kamiyama, N., Nakagawa, T., Kamiyama, T., Iseki, K., Tanaka, S., Todo, S."Type I interferon prolongs cell cycle progression via p21WAF1/CIP1 induction in human colon cancer cells". International Journal of Oncology 31, no. 3 (2007): 613-620. https://doi.org/10.3892/ijo.31.3.613