Prevalence of oral <em>Capnocytophaga</em> species and their association with dental plaque accumulation and periodontal inflammation in middle‑aged and older people

Shigeishi,Hideo; Hamada,Natsuki; Kaneyasu,Yoshino; Niitani,Yoshie; Takemoto,Toshinobu; Ohta,Kouji

doi:10.3892/br.2024.1787

Prevalence of oral Capnocytophaga species and their association with dental plaque accumulation and periodontal inflammation in middle‑aged and older people

Authors:
- Hideo Shigeishi
- Natsuki Hamada
- Yoshino Kaneyasu
- Yoshie Niitani
- Toshinobu Takemoto
- Kouji Ohta
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Affiliations: Department of Public Oral Health, Program of Oral Health Sciences, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima 734‑8553, Japan, Department of Oral Health Management, Program of Oral Health Sciences, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima 734‑8553, Japan
Published online on: April 30, 2024 https://doi.org/10.3892/br.2024.1787
Article Number: 99

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Abstract

Capnocytophaga species are commonly found in human oral microbiome. The aim of the present study was to understand the association of the prevalence of oral Capnocytophaga species with oral hygiene and periodontal inflammation. A total of 136 patients (median age 72 years) who visited the Hiroshima University Hospital (Hiroshima, Japan) between April 2021 and June 2023 were enrolled. Swab samples were obtained from the tongue surface. DNA from Capnocytophaga species (C. ochracea and C. sputigena) was detected by real‑time PCR analysis. Dental plaque accumulation was observed to assess the oral hygiene condition of participants. Additionally, clinical periodontal inflammation was assessed with periodontal inflamed surface area (PISA) scores. Clinical confounding factors such as age, sex, lifestyle‑related disease, remaining teeth and denture wearing between Capnocytophaga species‑positive and ‑negative groups were adjusted with a propensity score matching method. Mann‑Whitney U and χ2 or Fisher's exact test were employed for statistical analysis. The prevalence rate was 67.6% for oral C. ochracea and 83.1% for C. sputigena. C. ochracea‑positive participants showed significantly higher plaque control record scores (an indicator of dental plaque accumulation) than C. ochracea‑negative participants (P=0.03). Additionally, C. ochracea/C. sputigena dual‑positive participants exhibited significantly higher plaque control record and PISA scores than non‑dual‑positive participants (P=0.01 and P=0.04, respectively). Propensity score matching was conducted in the C. ochracea/C. sputigena dual‑positive group and the non‑dual‑positive group for adjustment of clinical factors, resulting in 51 matched patient pairs. C. ochracea/C. sputigena dual‑positive participants had significantly higher plaque control record scores than non‑dual‑positive participants (P=0.02). The present results suggest that the prevalence of both oral C. ochracea and C. sputigena is associated with poor oral hygiene in middle‑aged and older people.

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