Open Access

Changes in the intestinal microbiota of multiple myeloma patients living in high‑altitude and cold regions analyzed using 16s rRNA high‑throughput sequencing

  • Authors:
    • Xiaofei Liang
    • Xuyang Guo
    • Huixin Jin
    • Lijuan Shen
    • Ling Ding
    • Xin Guan
    • Yujie Kou
    • Yi Wu
    • Haipeng Guo
  • View Affiliations

  • Published online on: April 29, 2024     https://doi.org/10.3892/etm.2024.12557
  • Article Number: 269
  • Copyright: © Liang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Multiple myeloma (MM) is a plasma cell clonal disease and these plasma cells can survive in the gut. The intestinal microbiota is a complex ecosystem and its dysfunction can release persistent stimulus signals that trigger genetic mutations and clonal evolution in the gut. The present study analyzed the intestinal microbiota in fecal samples of MM patients in high‑altitude and cold regions of China using 16s rRNA sequencing and analyzed significantly enriched species at the phylum and genus levels. Although no significant difference in the alpha diversity was observed between the MM and control groups, a significant difference was noted in the beta diversity. A total of 15 significant differential bacteria at the genus level were found between the two groups, among which Bacteroides, Streptococcus, Lactobacillus and Alistipes were significantly enriched in the MM group. The present study also constructed a disease diagnosis model using Random Forest analysis and verified its accuracy using receiver operating characteristic analysis. In addition, using correlation analysis, it demonstrated that the composition of the intestinal microbiota in patients with MM was associated with complement levels. Notably, the present study predicted that the signaling and metabolic pathways of the intestinal microbiota affected MM progression through Kyoto Encyclopedia of Genes and Genomes functional analysis. The present study provides a new approach for the prevention and treatment of MM, in which the intestinal microbiota may become a novel therapeutic target for MM.
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June-2024
Volume 27 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Liang X, Guo X, Jin H, Shen L, Ding L, Guan X, Kou Y, Wu Y and Guo H: Changes in the intestinal microbiota of multiple myeloma patients living in high‑altitude and cold regions analyzed using 16s rRNA high‑throughput sequencing. Exp Ther Med 27: 269, 2024
APA
Liang, X., Guo, X., Jin, H., Shen, L., Ding, L., Guan, X. ... Guo, H. (2024). Changes in the intestinal microbiota of multiple myeloma patients living in high‑altitude and cold regions analyzed using 16s rRNA high‑throughput sequencing. Experimental and Therapeutic Medicine, 27, 269. https://doi.org/10.3892/etm.2024.12557
MLA
Liang, X., Guo, X., Jin, H., Shen, L., Ding, L., Guan, X., Kou, Y., Wu, Y., Guo, H."Changes in the intestinal microbiota of multiple myeloma patients living in high‑altitude and cold regions analyzed using 16s rRNA high‑throughput sequencing". Experimental and Therapeutic Medicine 27.6 (2024): 269.
Chicago
Liang, X., Guo, X., Jin, H., Shen, L., Ding, L., Guan, X., Kou, Y., Wu, Y., Guo, H."Changes in the intestinal microbiota of multiple myeloma patients living in high‑altitude and cold regions analyzed using 16s rRNA high‑throughput sequencing". Experimental and Therapeutic Medicine 27, no. 6 (2024): 269. https://doi.org/10.3892/etm.2024.12557