Expression signatures of long non-coding RNAs in early brain injury following experimental subarachnoid hemorrhage

Zheng,Bingjie; Liu,Huailei; Wang,Ruke; Xu,Shancai; Liu,Yaohua; Wang,Kaikai; Hou,Xu; Shen,Chen; Wu,Jianing; Chen,Xin; Wu,Pei; Zhang,Guang; Ji,Zhiyong; Wang,Hongyu; Xiao,Yao; Han,Jianyi; Shi,Huaizhang; Zhao,Shiguang

doi:10.3892/mmr.2015.3474

Expression signatures of long non-coding RNAs in early brain injury following experimental subarachnoid hemorrhage

Authors:
- Bingjie Zheng
- Huailei Liu
- Ruke Wang
- Shancai Xu
- Yaohua Liu
- Kaikai Wang
- Xu Hou
- Chen Shen
- Jianing Wu
- Xin Chen
- Pei Wu
- Guang Zhang
- Zhiyong Ji
- Hongyu Wang
- Yao Xiao
- Jianyi Han
- Huaizhang Shi
- Shiguang Zhao
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Affiliations: Department of Neurosurgery, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China
Published online on: March 11, 2015 https://doi.org/10.3892/mmr.2015.3474
Pages: 967-973
Copyright: © Zheng et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

Subarachnoid hemorrhage (SAH) is an important cause of mortality in stroke patients. Long non‑coding RNAs (LncRNAs) have important functions in brain disease, however their expression profiles in SAH remain to be elucidated. The present study aimed to investigate the expression signatures of LncRNAs and mRNAs in early brain injury (EBI) following SAH in a rat model. Male Wistar rats were randomly divided into an SAH group and a sham operation group. The expression signatures of the LncRNAs and mRNAs in the temporal lobe cortex were investigated using a rat LncRNAs array following experimental SAH. The results revealed that there were 144 downregulated and 64 upregulated LncRNAs and 181 downregulated and 221 upregulated mRNAs following SAH. Additionally, two upregulated (BC092207, MRuc008hvl) and three downregulated (XR_006756, MRAK038897, MRAK017168) LncRNAs were confirmed using reverse transcription quantitative polymerase chain reaction. The differentially expressed mRNAs were further analyzed using the Gene Ontology and the Kyoto Encyclopedia of Genes and Genomes (KEGG) databases. The pathway analysis results provided by the KEGG database indicated that eight pathways associated with inflammation were involved in EBI following SAH. In conclusion, these results demonstrated that the expression profiles of the LncRNAs and mRNAs were significantly different between the SAH‑induced EBI group and the sham operation group. These differently expressed LncRNAs may be important in EBI following SAH.

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