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Altered expression of hyperpolarization-activated cyclic nucleotide-gated channels and microRNA-1 and -133 in patients with age-associated atrial fibrillation

  • Authors:
    • Yao‑Dong Li
    • Yi‑Fan Hong
    • Yueerguli Yusufuaji
    • Bao‑Peng Tang
    • Xian‑Hui Zhou
    • Guo‑Jun Xu
    • Jin‑Xin Li
    • Lin Sun
    • Jiang‑Hua Zhang
    • Qiang Xin
    • Jian Xiong
    • Yu‑Tong Ji
    • Yu Zhang

  • View Affiliations

  • Published online on: May 25, 2015     https://doi.org/10.3892/mmr.2015.3831
  • Pages: 3243-3248

  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

Hyperpolarization-activated cyclic nucleotide-gated (HCN) cation channels mediate pacemaker currents in the atrium. The microRNA (miR) families miR-1 and miR-133 regulate the expression of multiple genes involved in myocardial function, including HCN channels. It was hypothesized that age‑dependent changes in HCN2, HCN4, miR‑1 and miR‑133 expression may contribute to age‑associated atrial fibrillation, and therefore the correlation between expression levels, among adult (≤65 years) and aged patients (≥65 years), and sinus rhythm was determined. Right atrial appendage samples were collected from 60 patients undergoing coronary artery bypass grafting. Reverse transcription-quantitative polymerase chain reaction (PCR) and western blot analyses were performed in order to determine target RNA and protein expression levels. Compared with aged patients with sinus rhythm, aged patients with atrial fibrillation exhibited significantly higher HCN2 and HCN4 channel mRNA and protein expression levels (P<0.05), but significantly lower expression levels of miR‑1 and miR‑133 (P<0.05). In addition, aged patients with sinus rhythm exhibited significantly higher expression levels of HCN2 and HCN4 channel mRNA and protein (P<0.05), but significantly lower expression levels of miR‑1 and ‑133 (P<0.05), compared with those of adult patients with sinus rhythm. Expression levels of HCN2 and HCN4 increased with age, and a greater increase was identified in patients with age‑associated atrial fibrillation compared with that in those with aged sinus rhythm. These electrophysiological changes may contribute to the induction of ectopic premature beats that trigger atrial fibrillation.
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September-2015
Volume 12 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

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  • Impact Factor: 3.4
  • Ranked Medicine Research and Experimental
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  • CiteScore: 6.9
  • CiteScore Rank: Q2: #98/328 Biochemistry, Genetics and Molecular Biology
  • Source Normalized Impact per Paper (SNIP): 0.721
  • SCImago Journal Rank (SJR): 0.65
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Spandidos Publications style
Li YD, Hong YF, Yusufuaji Y, Tang BP, Zhou XH, Xu GJ, Li JX, Sun L, Zhang JH, Xin Q, Xin Q, et al: Altered expression of hyperpolarization-activated cyclic nucleotide-gated channels and microRNA-1 and -133 in patients with age-associated atrial fibrillation. Mol Med Rep 12: 3243-3248, 2015
APA
Li, Y., Hong, Y., Yusufuaji, Y., Tang, B., Zhou, X., Xu, G. ... Zhang, Y. (2015). Altered expression of hyperpolarization-activated cyclic nucleotide-gated channels and microRNA-1 and -133 in patients with age-associated atrial fibrillation. Molecular Medicine Reports, 12, 3243-3248. https://doi.org/10.3892/mmr.2015.3831
MLA
Li, Y., Hong, Y., Yusufuaji, Y., Tang, B., Zhou, X., Xu, G., Li, J., Sun, L., Zhang, J., Xin, Q., Xiong, J., Ji, Y., Zhang, Y."Altered expression of hyperpolarization-activated cyclic nucleotide-gated channels and microRNA-1 and -133 in patients with age-associated atrial fibrillation". Molecular Medicine Reports 12.3 (2015): 3243-3248.
Chicago
Li, Y., Hong, Y., Yusufuaji, Y., Tang, B., Zhou, X., Xu, G., Li, J., Sun, L., Zhang, J., Xin, Q., Xiong, J., Ji, Y., Zhang, Y."Altered expression of hyperpolarization-activated cyclic nucleotide-gated channels and microRNA-1 and -133 in patients with age-associated atrial fibrillation". Molecular Medicine Reports 12, no. 3 (2015): 3243-3248. https://doi.org/10.3892/mmr.2015.3831