Open Access

Drug resistance‑related gene targets and molecular mechanisms in the A2780/Taxol‑resistant epithelial ovarian cancer cell line

  • Authors:
    • Ruihui Yang
    • Huainian Zhang
    • Zexin Chen
    • Tao Zhang
    • Peng Wei
    • Huaguo Liang
    • Yaoyao He
    • Changtao Zheng
    • Xicheng Wang
    • Yongli Zhang
  • View Affiliations

  • Published online on: March 26, 2024     https://doi.org/10.3892/ol.2024.14365
  • Article Number: 232
  • Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Epithelial ovarian cancer (EOC) is a fatal gynecological malignant tumor with a low 5‑year survival rate. The use of the first‑line chemotherapeutic drug, paclitaxel, for the treatment of EOC is associated with resistance, often leading to treatment failure. The present study investigated the gene targets in an A2780 paclitaxel‑resistant EOC cell line (A2780/Taxol), and the potential underlying mechanisms using transcriptome sequencing technology and bioinformatics analysis. The transcriptome of the A2780/Taxol cell line was sequenced, and 498 differentially expressed genes were obtained contained in the Gene Expression Omnibus dataset. Further bioinformatics analysis revealed that matrix metalloproteinase 1 (MMP1), zyxin (ZYX) and Unc‑5 netrin receptor C (UNC5C) may be gene targets related to paclitaxel resistance. Moreover, Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis indicated that a potential mechanism associated with paclitaxel resistance was related to cell migration. Furthermore, the expression levels of MMP1, ZYX and UNC5C were verified using western blotting, immunofluorescence and immunohistochemistry in vitro. The results revealed that the expression levels of MMP1 and ZYX were significantly increased in A2780/Taxol cells, while UNC5C expression was significantly decreased, which was consistent with the results of the transcriptome sequencing. The present study demonstrated that MMP1, ZYX and UNC5C may be the gene targets associated with paclitaxel resistance in EOC. These genes have potential to be used as molecular markers for EOC drug therapy, targeted elimination of drug resistance, and evaluation of treatment efficacy and patient prognosis.
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May-2024
Volume 27 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Yang R, Zhang H, Chen Z, Zhang T, Wei P, Liang H, He Y, Zheng C, Wang X, Zhang Y, Zhang Y, et al: Drug resistance‑related gene targets and molecular mechanisms in the A2780/Taxol‑resistant epithelial ovarian cancer cell line. Oncol Lett 27: 232, 2024
APA
Yang, R., Zhang, H., Chen, Z., Zhang, T., Wei, P., Liang, H. ... Zhang, Y. (2024). Drug resistance‑related gene targets and molecular mechanisms in the A2780/Taxol‑resistant epithelial ovarian cancer cell line. Oncology Letters, 27, 232. https://doi.org/10.3892/ol.2024.14365
MLA
Yang, R., Zhang, H., Chen, Z., Zhang, T., Wei, P., Liang, H., He, Y., Zheng, C., Wang, X., Zhang, Y."Drug resistance‑related gene targets and molecular mechanisms in the A2780/Taxol‑resistant epithelial ovarian cancer cell line". Oncology Letters 27.5 (2024): 232.
Chicago
Yang, R., Zhang, H., Chen, Z., Zhang, T., Wei, P., Liang, H., He, Y., Zheng, C., Wang, X., Zhang, Y."Drug resistance‑related gene targets and molecular mechanisms in the A2780/Taxol‑resistant epithelial ovarian cancer cell line". Oncology Letters 27, no. 5 (2024): 232. https://doi.org/10.3892/ol.2024.14365