This article is mentioned in:

Introduction

Hepatitis B virus (HBV) infection is prevalent around the world, and is one of the most severe infectious diseases in China. An epidemiological survey of hepatitis B in 2006 demonstrated that 7.18% of the Chinese population aged 1–59 years carried the hepatitis B surface antigen (HBsAg) (1). Accordingly, ~93 million individuals in China suffer from chronic HBV infection (2). HBV infection can cause a series of pathological changes in the liver, including mild to severe liver fibrosis and severe hepatitis. These lesions can progress to cirrhosis, liver failure and end-stage liver disease (3).

Liver acute or chronic inflammation is frequently accompanied by the formation and development of liver fibrosis (4–6). The clinical manifestations of liver fibrosis and cirrhosis are different. Mild liver fibrosis can be completely asymptomatic, while severe liver fibrosis can be manifested as hepatic failure. Similarly, early stage cirrhosis has no evident symptoms or no specific clinical symptoms; however, advanced liver cirrhosis has multisystem manifestations of hepatic decomposition, including hemorrhage, infection, hepatic encephalopathy and carcinogenesis (3,7). The 5-year mortality rate of early cirrhosis is <20% and the 10-year survival rate is ~70%; by contrast, the 5-year survival rate of decompensated cirrhosis is <50% (8). Thus, the timely diagnosis of liver fibrosis has an important value in treatment and prognostic assessment of chronic liver disease.

Transient elastography, also known as Fibroscan, is a new, noninvasive, indolent, fast and objectively quantitative method for evaluating liver fibrosis by measuring the liver stiffness (9). Castéra et al (9) and Colletta et al (10) have reported that detecting liver fibrosis by Fibroscan resulted in 84–95% of patients not requiring liver biopsy in 2005. Fibroscan has previously been used to evaluate the degree of liver fibrosis in patients with hepatitis B (11), hepatitis C (12,13) cirrhosis (14), alcoholic liver disease (15,16) and autoimmune liver diseases (17). It is also used in antiviral therapy efficacy evaluation (18,19).

The aim of the present study was to investigate the independent predictors affecting Fibroscan detection of liver fibrosis, and the method of multiple regression analysis was used to determine these predictors.

Materials and methods

Clinical data of subjects

A total of 181 cases were enrolled into the present study. These patients were diagnosed with chronic hepatitis, cirrhosis, fatty liver, autoimmune hepatitis or non-specific cholangitis by liver biopsy in the Outpatient and Inpatient Departments of the Jinan Infectious Disease Hospital (Jinan, China) during the period between June 2011 and June 2013. These cases included 128 male patients and 53 female patients, with ages ranging between 17 and 72 years and an mean age of 38.7±10.8 years. Of the 181 included patients, 154 cases were HBsAg-positive. The present study was conducted in accordance with the Declaration of Helsinki, and with approval from the Ethics Committee of Qianfoshan Hospital, Shandong University (Jinan, China). Written informed consent was obtained from all participants.

Clinical information and biochemical examination

The analyzed clinical and biochemical indexes included the following: Gender, age, body-mass index (BMI), platelet (PLT), alanine aminotransferase (ALT), aspartate aminotransferase (AST), γ-glutamyl transpeptidase (GGT), alkaline phosphatase (ALP), serum albumin (ALB), globulin (GLOB), ALB/GLOB ratio (A/G), total bilirubin (BILT), direct bilirubin (DBIL), indirect bilirubin (IBIL), cholinesterase (CHEX), prothrombin time (PT), prothrombin activity (PTA) and fibrinogen (FIB). The BMI was calculated according to the following formula: BMI = body weight (kg) / height2 (m2). In addition, fasting blood was collected from all patients for biochemical analysis. PLT level was analyzed by an ADVIA 2120 hematology analyzer (Bayer Diagnostics, Tarrytown, NY, USA). The levels of ALT, AST, GGT, ALP, ALB, GLOB, A/G, BILT, DBIL, IBIL and CHEX were detected with an Abbott Architect c8000 biochemical analyzer (Abbott Diagnostics, Santa Clara, CA, USA). Finally, the PT, PTA and FIB levels were analyzed using a STAGO Compact automatic coagulation analyzer (Diagnostica Stago, Asnières-sur-Seine, France).

Fibroscan

Liver stiffness in the 181 patients was measured by Fibroscan (Echosens, Paris, France) prior to the liver biopsy on the same day. Briefly, the probe was place at the detection area from the anterior axillary line on the right to the 7th, 8th and 9th intercostal space in the midaxillary line for continuous detection. A total of 10 successful detections were performed in each patient. The results were indicated by liver stiffness measurement (LSM) in kPa. These measurements were considered to be reliable only when the interquartile range was <1/3 of the median, and the success rate (times of successful capture of echoes / total emitting times) was ≥60%. The detection was considered as failed in cases when the detection value was not obtained.

Statistical analysis

All the data were analyzed by SPSS version 13.0 software (SPSS, Inc., Chicago, IL, USA). The correlation between LSM and the variables was analyzed by Spearman's correlation. Multiple regression analysis was also performed to analyze the independent predictors affecting Fibroscan detection and to obtain the multiple regression equation. Differences with P<0.05 were considered as statistically significant.

Results

Clinical features of patients

As shown in Table I, the pathological results of the 181 cases showed that patients with hepatitis B accounted for 85.1% of all included cases and patients with autoimmune diseases (including autoimmune hepatitis, primary biliary cirrhosis and primary sclerosing cholangitis) accounted for 9.9%. The proportion of patients with hepatitis C (1.1%), fatty liver (1.7%) and non-specific liver damage (2.2%) was relatively small. The LSM value ranged between 2.5 and 75.0 kPa, with the mean value of 11.9±11.9 kPa. Biochemical indexes are also shown in Table I, including BMI, PLT, ALT, AST, GGT, ALP, ALB, GLOB, A/G, BILT, DBIL, IBIL, CHEX, PT, PTA and FIB. In the majority of patients, BMI, PLT, ALP, GLOB, ALB/GLOB ratio, BILT, DBIL, IBIL, CHEX and FIB were normal; in more than half of total patients, ALT and/or AST and/or GGT were elevated.

Table I. Clinical features of patients (n=181).

Table I.

Clinical features of patients (n=181).

Index	Value	Normal range
Male to female ratio [n (%)]	128:53 (70.7%:29.3%)
Age (years)	38.7±10.8 (17–72)
BMI (kg/m2)	24.3±3.3 (16.5–34.4)	18.5–24.99
Liver stiffness measurement (kPa)	11.9±11.9 (2.5–75.0)
Pathological results [n (%)]
Hepatitis B	154 (85.1)
Hepatitis C	2 (1.1)
Autoimmune hepatitis	18 (9.9)
Fatty liver	3 (1.7)
Non-specific liver damage	4 (2.2)
PLT(×103/mm3)	187.4±67.6 (54–388)	100–300
ALT (IU/l)	65.9±69.9 (7–548)	0–40.0
AST (IU/l)	49.5±48.9 (12–341)	0–40.0
GGT (IU/l)	72.7±92.9 (9–504)	12.0–64.0
ALP (IU/l)	82.0±71.0 (9–636)	40.0–150.0
ALB (g/l)	44.2±4.5 (28.4–55.0)	35.0–55.0
GLOB (g/l)	31.6±5.7 (19.7–60.9)	25.0–35.0
A/G ratio	1.4±0.3 (0.6–2.2)	1.2–35.0
BILT (mg/l)	25.9±46.9 (5.3–535.3)	4.0–25.0
DBIL (mg/l)	9.9±27.7 (1.7–301.8)	1.0–10.0
IBIL (mg/l)	15.9±20.0 (3.3–233.5)	1.0–20.0
CHEX (U/l)	289.4±85.7 (78–548)	130.0–310.0
PT (sec)	13.9±1.4 (11.3–19.2)	8.8–13.8
PTA (%)	90.3±16.4 (51–145)	70.0–130.0
FIB (g/l)	2.9±1.6 (1.5–21.4)	2.0–4.0

[i] Numerical variable data are shown as the mean ± standard deviation (range), unless otherwise stated. BMI, body-mass index; PLT, platelet; ALT, alanine aminotransferase; AST, aspartate aminotransferase; GGT, γ-glutamyl transpeptidase; ALP, alkaline phosphatase; ALB, serum albumin; GLOB, globulin; A/G, albumin/globulin; BILT, total bilirubin; DBIL, direct bilirubin; IBIL, indirect bilirubin; CHEX, cholinesterase; PT, prothrombin time; PTA, prothrombin activity; FIB, fibrinogen.

Correlation analysis

To determine the association between the LSM and the clinical indexes of patients, Spearman's correlation analysis was performed. As shown in Table II, the factors of age, BMI, AST, GGT, ALP, GLOB, BILT, DBIL, IBIL and PT were positively corrected with LSM (P<0.05). By contrast, the factors of PLT, ALB, A/G, CHEX and PTA were negatively corrected with LSM (P<0.05). However, there was no correlation between ALT and LSM, or between FIB and LSM. These results suggest that the factors of age, BMI, AST, GGT, ALP, GLOB, BILT, DBIL, IBIL, PT, PLT, ALB, A/G, CHEX and PTA are correlated with LSM, and that they may affect the detection results of Fibroscan.

Table II. Correlation analysis between liver stiffness measurement and various indexes.

Table II.

Correlation analysis between liver stiffness measurement and various indexes.

Index	SCC	P-value
Age	0.307	<0.001
BMI	0.160	0.035
PLT	−0.295	<0.001
ALT	0.060	0.435
AST	0.274	<0.001
GGT	0.373	<0.001
ALP	0.378	<0.001
ALB	−0.507	<0.001
GLOB	0.269	<0.001
A/G ratio	−0.414	<0.001
BILT	0.267	<0.001
DBIL	0.291	<0.001
IBIL	0.222	0.004
CHEX	−0.399	<0.001
PT	0.324	<0.001
PTA	−0.282	<0.001
FIB	0.005	0.946

[i] SCC, Spearman's correlation coefficient; BMI, body-mass index; PLT, platelet; ALT, alanine aminotransferase; AST, aspartate aminotransferase; GGT, γ-glutamyl transpeptidase; ALP, alkaline phosphatase; ALB, serum albumin; GLOB, globulin; A/G, albumin/globulin; BILT, total bilirubin; DBIL, direct bilirubin; IBIL, indirect bilirubin; CHEX, cholinesterase; PT, prothrombin time; PTA, prothrombin activity; FIB, fibrinogen.

Multiple regression analysis

To determine the independent affecting factors of Fibroscan detection, multiple regression analysis was conducted. Collinearity diagnosis among variables was performed prior to analysis. As shown in Table III, there was collinearity among ALB, GLOB and A/G, among TBIL, DBIL and IBIL, and among PT and PTA. In addition, this collinearity had a negative influence on the stability of the regression equation. Therefore, the variables of GLOB, A/G, DBIL, IBIL and PT were removed from multiple regression analysis. Multiple regression analysis was then performed on the indexes of age, BMI, PLT, AST, GGT, ALP, ALB, TBIL, PTA and CHEX. Table III demonstrates that the effects of PLT, ALB, PTA and BMI on LSM were statistically significant (all P<0.05). The tolerance and variance inflation factors in the table all showed that there was no collinearity among these indicators, and the constant terms were significant. Thus, PLT, ALB, PTA and BMI were found to be independent predictors of LSM. Therefore, the regression equation was as follow: Y=47.087 - 0.244X1 - 0.217X2 - 0.205X3 + 0.163X4, where X1 is PLT (in ×103/mm3), X2 is ALB (in g/l), X3 is PTA (in %) and X4 is BMI (in kg/m2).

Table III. Multiple regression analysis results of liver stiffness measurement influencing factors.

Table III.

Multiple regression analysis results of liver stiffness measurement influencing factors.

	Unstandardized coefficients		Standardized coefficients	Collinearity diagnostics
Factor	B-value	Standard error	Beta value	t-test	P-value	Tolerance	VIFs
Constant	47.087	15.420		3.054	0.003
Age	−0.014	0.091	−0.012	−0.149	0.882	0.740	1.351
BMI	0.542	0.252	0.163	2.150	0.033	0.786	1.271
PLT	−0.046	0.014	−0.244	−3.278	0.001	0.813	1.230
AST	−0.004	0.023	−0.013	−0.159	0.874	0.638	1.568
GGT	0.022	0.013	0.178	1.634	0.105	0.378	2.647
ALP	0.026	0.016	0.159	1.593	0.114	0.452	2.211
ALB	−0.594	0.281	−0.217	−2.113	0.037	0.427	2.340
CHEX	−0.008	0.015	−0.056	−0.553	0.581	0.432	2.313
PTA	−0.165	0.061	−0.205	−2.697	0.008	0.782	1.280
BILT	0.026	0.019	0.109	1.390	0.167	0.729	1.371

[i] VIF, variance inflation factor; BMI, body-mass index; PLT, platelet; AST, aspartate aminotransferase; GGT, γ-glutamyl transpeptidase; ALP, alkaline phosphatase; ALB, serum albumin; CHEX, cholinesterase; PTA, prothrombin activity; BILT, total bilirubin.

These results suggest that the contribution of these four predictors to the regression equation was in the following descending order: PLT (negative correlation) > ALB (negative correlation) > PTA (negative correlation) > BMI (positive correlation).

Discussion

Fibroscan is a non-invasive method for assessing liver stiffness, which is used as an alternative method to liver biopsy. Marcellin et al (20) reported that Fibroscan was reliable for the detection of significant fibrosis or cirrhosis in HBV patients. A previous meta-analysis (21) concluded that Fibroscan theoretically has good sensitivity and specificity for cirrhosis, but it should be used with caution. In addition, Sagir et al (22) observed that the LSM value increased in patients with acute liver injury, even in cases with no evident liver fibrosis, suggesting that Fibroscan is not suitable for detecting cirrhosis/fibrosis in these patients. These previous findings indicated that there are various factors affecting Fibroscan detection.

In the present study, the factors influencing Fibroscan detection were analyzed. The correlation analysis demonstrated that the correlation of LSM with the indexes FIB and ALT was poor, indicating that FIB and ALT may not affect the Fibroscan detection. Subsequently, multiple regression analysis was performed in the current study. Since the results of multiple regression analysis are different if different indexes are introduced, collinearity diagnosis among the included variables was performed. A common problem in multiple regression analysis is the evident linear relation among numerous independent variables. This problem may result in an incorrect regression equation, such as an extremely unstable estimated value, sensitivity to the independent variables included in the equation, or incorrect positivity or negativity of the estimated value (23). Thus, diagnosis on multicollinearity was performed prior to estimating the multiple regression equation in the present study. According to the results, the following indexes were removed based on the collinearity diagnosis: GLOB, A/G, DBIL, IBIL and PT. Thus, the age, BMI, PLT, AST, GGT, ALP, ALB, TBIL, PTA and CHEX were selected for further analysis. Through multiple regression analysis, the four factors PLT, ALB, PTA and BMI were found to be independent predictors and were entered into the equation. The standardized coefficients listed in Table III showed that the contribution of these four predictors to the equation was as follows: PLT (negative correlation) > ALB (negative correlation) > PTA (negative correlation) > BMI (positive correlation).

Although PLTs are mainly produced by the megakaryocytes of mature myeloid cells, they are also regulated by thrombopoietin produced by hepatocytes. With the evolvement of liver tissue fibrosis, thrombopoietin levels decrease, while PLT production is reduced (24,25). It is well known that the liver is the only human organ that synthesizes ALB. When the liver cells are necrotized due to various causes and are replaced by fibrous tissue, the ALB level decreases. Therefore, ALB is a sensitive index evaluating the synthetic function of liver cells and is also an important index for liver cirrhosis diagnosis. All blood coagulation factors with the exception of calcium ions and factor VIIa, are synthesized in the liver. The decrease of PTA activity and the aggravation of liver cell damage are associated with the decrease of absorbing barrier of vitamin K for heparin inactivation. In obese individuals, fatty liver will develop into liver fibrosis (26), and the thickness of fat layer on the abdominal wall may lead to overestimation of the LSM value (27).

The results of the present study regarding ALT and AST were inconsistent with a previous report (28). Tapper et al (28) showed that in patients with HCV infection and early-stage fibrosis, increased levels of ALT correlated with liver stiffness among patients in the lowest strata of fibrosis (METAVIR scores 0–2). The present study showed different results. In 181 patients, with 154 cases positive in HbsAg, ALT and AST had no statistically significant association with LSM. In the present study, ALT and FIB were removed from the correlation analysis, and then AST was removed from the multiple regression analysis. However, if ALT or AST were included in the regression analysis, the final calculation result would still be satisfactory, since the effect of ALT or AST on LSM is relatively small when there are more independent variables.

In conclusion, the findings of the present study indicated that when detecting the liver stiffness by Fibroscan, the parameters PLT, ALB, PTA and BMI may affect the detection results. Therefore, the diagnosis and evaluation of liver fibrosis should comprehensively consider the results of Fibroscan along with the clinical and laboratory examination results. Evaluating the degree of liver fibrosis simply based on the LSM value is not accurate.

References