Open Access

Infectious mononucleosis and hepatic function

  • Authors:
    • Li Zhang
    • Pingping Zhou
    • Zhaowei Meng
    • Chongjie Pang
    • Lu Gong
    • Qing Zhang
    • Qiyu Jia
    • Kun Song
  • View Affiliations

  • Published online on: January 10, 2018     https://doi.org/10.3892/etm.2018.5736
  • Pages: 2901-2909
  • Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Abnormal hepatic function is common in infectious mononucleosis (IM). However, it remains unknown why increased transferase levels are more common than bilirubin abnormalities in IM. The current study aimed to investigate these associations in the Chinese population. A total of 95 patients with IM (47 males and 48 females) were enrolled in the current study, as well as 95 healthy controls. Patients were sorted by sex. A receiver operating characteristic (ROC) curve was used to determine cut‑off values for IM diagnosis and prediction. Crude and adjusted odds ratios (OR) for IM were analyzed using binary logistic regression. It was determined that alanine aminotransferase (ALT), aspartate aminotransferase (AST) and γ‑glutamyl transferase (GGT) levels were significantly higher in patients with IM compared with controls; however, total bilirubin (TB) levels were significantly lower in patients with IM. ROCs demonstrated that, if ALT, AST and GGT concentrations were higher than, or if TB was lower than, cut‑off values, they were predictive of IM. Binary logistic regression identified that the risk of IM in patients exhibiting high levels of transferases was significantly increased, particularly in males. Crude ORs in ALT quartile 4 were 21.667 and 10.111 for males and females, respectively and adjusted ORs were 38.054 and 9.882, respectively. A significant IM risk of IM was evident in patients with low bilirubin levels and females appeared to be particularly susceptible. For example, crude ORs in quartile 1 were 8.229 and 8.257 for males and females, respectively and adjusted ORs were 8.883 and 10.048, respectively. Therefore, the current study identified a positive association between transferase levels and IM and a negative association between TB and IM. Therefore, the results of the current study indicate that high transferases are suggestive of IM, particularly in males, whereas low TB is suggestive for IM, particularly in females.

Introduction

Infectious mononucleosis (IM) is a common disease associated with Epstein-Barr virus (EBV) infection and ~5 cases occur per 1,000 people every year (1,2) The results of a survey conducted in Beijing indicated that the incidence of IM peaks among preschool children and young people aged 15–24 years old (3). Diagnosis of IM is generally based on identifying the triad of clinical manifestations of fever, pharyngitis and lymphadenopathy in patients, as well as a positive result following a serological test for EBV (1,2). However, patients with IM also exhibit other symptoms, including headache, fatigue, exanthema, hepatomegaly and splenomegaly (4). The majority of patients with IM recover without complications and return to their normal activities ~2 months following disease onset (1,2). However, severe complications, including upper-airway obstruction, hemolytic anemia, thrombocytopenia, hepatitis, myocarditis, splenic rupture, neurological and hematological complications may occur in certain cases (1,2). The treatment for IM is generally supportive and only alleviates symptoms, as EBV cannot be cured; these treatments include glucose and sodium chloride intravenous infusion, reduced glutathione intravenous infusion, pantoprazole sodium injection, and oral loxoprofen and bicyclol tablets intake (1,2). Abnormality of hepatic function is commonly encountered in IM; however, it has been demonstrated for many years that increases in alanine aminotransferase (ALT), aspartate aminotransferase (AST) and γ-glutamyl transferase (GGT) occur more frequently in IM than bilirubin abnormalities (112). However, the underlying reasons for these elevations remain unknown. The results of previous studies demonstrated that >80% of patients with IM presented with abnormal transferase levels, particularly ALT, but jaundice was uncommon (1,2,13). In addition, Hu et al (14) indicated that the presence of atypical lymphocytes and transaminase may be regarded as a diagnostic marker of IM.

The aim of the current study was to analyze the associations between hepatic function parameters and IM in a comprehensive manner. The aim was to identify whether abnormal hepatic function could be indicative of IM and whether there were any significant differences between hepatic function parameters between males and females.

Patients and methods

Patients

Between December 2014 and December 2015, 95 patients (mean age, 39.36 years; 47 males and 48 females) with a confirmed diagnosis of IM were enrolled in the Department of Infectious Diseases, Tianjin Medical University General Hospital (Tianjin, China). Patients with pre-existing liver diseases, including viral hepatitis, fatty liver disease, alcoholic liver disease and medication-induced liver damage, were excluded from the current study. During the same period, a normal control cohort consisting of 95 healthy participants (mean age, 39.45 years; 47 males and 48 females) was recruited from the Health Management Department, Tianjin Medical General University. None of the healthy participants had been diagnosed with any diseases and had attended Tianjin Medical General University for an annual health checkup. The ethical, methodological and protocol aspects of the current investigation were approved by the Institutional Review Board of Tianjin Medical University General Hospital and written informed consent was provided by all participants.

Parameter measurements

Following admittance of patients with IM to the Department of Infectious Diseases, blood tests and anthropometric measurements were conducted. For participants in the control group, blood tests and anthropometric measurements were completed during their visit to the hospital. Physical examinations included measurements of body height (BH) and body weight (BW). Subsequently, body mass index (BMI; kg/m2) was calculated using the following formula: BW/(BH2). Fasting blood tests were performed following venipuncture and the following serological parameters were measured: White blood cell (WBC), red blood cell (RBC), hemoglobin (Hg) and platelet (PLT) levels. A total of 3 ml of whole blood samples were collected and analyzed using a hemocytometer analyzer (Sysmex Corporation, Kobe, Japan). ALT, AST, GGT, total bilirubin (TB), blood urea nitrogen (BUN) and creatinine (Cr) levels were measured using the Hitachi Model 7170 analyzer (Hitachi Ltd., Tokyo, Japan). Levels of immunoglobulin (Ig)M and IgG antibodies against specific EBV antigens were measured by ELISA using three commercial kits (viral capsid antigen antibody IgM, cat. no. E170606AK; viral capsid antigen antibody IgG, cat. no. E170706BO; viral nuclear antigen antibody IgG, cat. no. E170706BR) supplied by (Euroimmun; Medizinische Labordiagnostika AG, Lübeck, Germany). Ultrasonography on neck lymph nodes and abdominal organs was performed the using PHILIPS HD11 XE (Philips, Amsterdam, The Netherlands).

Diagnostic criteria

IM was generally diagnosed based on clinical presentations of a fever, pharyngitis, lymphadenopathy, the presence of atypical lymphocytes on a peripheral blood smear and a positive heterophile antibody test. The majority of the included patients referred to Tianjin Medical University General Hospital (Tianjin, China) were diagnosed with IM in local hospitals prior to recruitment. In order to establish definite IM diagnosis, serological testing for the identification of antibodies against specific EBV antigens was required by the aforementioned ELISA kits (1,2).

Statistical analysis

All data for males and females were analyzed separately and presented as either the mean ± standard deviation or the median (lower quartile, upper quartile). Differences of parameters between groups were measured using an independent samples T test or a Mann-Whitney test. Pearson's bivariate correlation analysis was performed between hepatic function indices and other variables. Receiver operating characteristic (ROC) curves were constructed and diagnostic efficacies were determined using optimal sensitivity and specificity values calculated from hepatic function indices. To compare ROC curves, a previously described protocol was used (15). By stratifying data with quartiles of hepatic function indices, the odds ratio (OR) for IM with a 95% confidence interval (CI) was calculated using binary logistic regression models. OR adjustments were completed by using age and BMI as covariates in a logistic regression model. The Statistical Package for Social Sciences (SPSS v17.0, Chicago, IL, USA) was used for statistical analysis and P<0.05 was determined to indicate a significant difference.

Results

Patient clinicopathological characteristics

Parameters were compared in males and females separately (Tables I and II). In males, WBC, ALT, AST and GGT were significantly higher in patients with IM than in healthy controls (Table I). However, RBC, Hg and TB were significantly lower in males with IM compared with controls. In females, ALT, AST and GGT were significantly higher in females with IM than in controls (Table II). However, RBC, Hg, TB, BUN and Cr were significantly lower in females with IM compared with controls.

Table I.

Parameter characteristics in males.

Table I.

Parameter characteristics in males.

ParametersPatients with IMControlsT value/Z value
Case no.4747
Age (years)37.40±16.8337.68±16.83−0.080
BMI (kg/m2)23.58±3.0224.74±3.50−1.725
WBC (×109/l)7.17±3.815.93±1.492.085a
RBC (×1012/l)4.46±0.515.19±0.38−7.870b
Hg (g/l)133.19±14.59155.15±10.21−8.453b
PLT (×109/l)208.47±80.43214.81±46.92−0.467
ALT (U/l)50.00 (22.00, 107.00)19.00 (14.00, 27.00)4.411b
AST (U/l)31.00 (19.00, 63.00)18.00 (14.00, 22.00)4.216a
GGT (U/l)57.00 (25.00, 123.00)20.00 (16.00, 29.00)5.164b
TB (µmol/l)9.50 (7.10, 11.90)11.70 (9.90, 17.90)2.934b
BUN (mmol/l)4.10±1.744.56±1.22−1.496
Cr (µmol/l)76.30±28.9283.34±12.75−1.528

{ label (or @symbol) needed for fn[@id='tfn1-etm-0-0-5736'] } Data presented as mean ± standard deviation or median (lower quartile, upper quartile).

a P<0.05

b P<0.01 (analyzed by independent sample's t test or Mann-Whitney test). IM, infectious mononucleosis; BMI, body mass index; WBC, white blood cell; RBC, red blood cell; Hg, hemoglobin; PLT, platelet; ALT, alanine aminotransferase; AST, aspartate aminotransferase; GGT, γ-glutamyl transferase; TB, total bilirubin; BUN, blood urea nitrogen; Cr, creatinine.

Table II.

Parameter characteristics in females.

Table II.

Parameter characteristics in females.

ParametersPatients with IMControlsT value
Case no.4848
Age (years)41.27±17.5441.19±17.350.023
BMI (kg/m2)23.52±3.8122.60±2.941.314
WBC (×109/l)6.29±3.625.17±1.541.975
RBC (×1012/l)3.93±0.434.44±0.27−6.954a
Hg (g/l)113.54±13.34130.73±8.49−7.530a
PLT (×109/l)235.65±90.10226.15±50.150.638
ALT (U/l)20.00 (12.00, 68.50)13.00 (9.25, 15.75)3.342a
AST (U/l)21.50 (16.00, 49.00)15.50 (13.00, 18.75)4.096a
GGT (U/l)25.50 (13.00, 49.50)11.00 (10.00, 16.75)4.101a
TB (µmol/l)6.10 (4.90, 7.55)9.70 (7.65, 12.28)4.379a
BUN (mmol/l)2.97±1.054.15±1.15−5.242a
Cr (µmol/l)52.13±9.4660.77±9.59−4.446a

{ label (or @symbol) needed for fn[@id='tfn4-etm-0-0-5736'] } Data presented as mean ± standard deviation or median (lower quartile, upper quartile).

a P<0.01 (analyzed by independent sample's t test or Mann-Whitney test). IM, infectious mononucleosis; BMI, body mass index; WBC, white blood cell; RBC, red blood cell; Hg, hemoglobin; PLT, platelet; ALT, alanine aminotransferase; AST, aspartate aminotransferase; GGT, γ-glutamyl transferase; TB, total bilirubin; BUN, blood urea nitrogen; Cr, creatinine.

Correlations between key variables

Correlation coefficients between hepatic function indices and other parameters were calculated in patients with IM (Table III). In males, ALT was positively correlated with WBC, AST and GGT, whereas in females, ALT was positively correlated with AST and GGT. AST was positively correlated with WBC, ALT and GGT in males and positively correlated with ALT and GGT in females. GGT was positively correlated with WBC, ALT and AST in males and positively correlated with age, BMI, WBC, ALT and AST in females. In males, TB only exhibited a positive correlation with Hg; however in females, TB was positively correlated with RBC, Hg and Cr.

Table III.

Pearson bivariate correlations in males and females.

Table III.

Pearson bivariate correlations in males and females.

Correlation coefficients in malesCorrelation coefficients in females


ParametersALTASTGGTTBALTASTGGTTB
Age−0.242a−0.175−0.041−0.072−0.132−0.0790.232a0.007
BMI0.0170.0080.047−0.011−0.0050.0430.355b0.122
WBC0.453b0.216a0.427b0.0900.1290.1150.232a−0.097
RBC−0.129−0.097−0.307b0.167−0.228a−0.232a−0.0990.243a
Hg−0.166−0.152−0.291b0.235a−0.134−0.149−0.0470.337b
PLT−0.086−0.132−0.0320.088−0.291b−0.306b0.058−0.258a
ALT0.850b0.672b0.0020.942b0.403b0.001
AST0.850b0.534b−0.0360.942b0.426b−0.003
GGT0.672b0.534b0.0800.403b0.426b0.161
TB0.002−0.0360.0800.001−0.0030.161
BUN−0.237a−0.209a−0.031−0.048−0.205a−0.240a−0.0210.195
Cr−0.082−0.0750.1310.127−0.084−0.1010.0280.284b

a P<0.05

b P<0.01. ALT, alanine aminotransferase; AST, aspartate aminotransferase; GGT, γ-glutamyl transferase; TB, total bilirubin; BMI, body mass index; WBC, white blood cell; RBC, red blood cell; Hg, hemoglobin; PLT, platelet; BUN, blood urea nitrogen; Cr, creatinine.

Diagnostic or predicative values of hepatic function indices for IM

From ROC analyses, all hepatic function indices exhibited significant diagnostic or predicative values for IM (Fig. 1 and Table IV). If ALT, AST and GGT concentrations were higher than the cut-off values of 27.5, 21.5 and 29.5 U/l in males, diagnostic accuracies of IM were 74.468, 71.277 and 75.532%, respectively. In females, the cut-off values for ALT, AST and GGT were 15.5, 17.5 and 18.0 U/l and diagnostic accuracies of IM were 67.708, 66.667 and 72.917%, respectively. If TB concentrations were lower than the cut-off values of 10.70 µmol/l in males and 7.45 µmol/l in females, diagnostic accuracies were 64.89 and 78.125%, respectively. Comparison of ROC curves in males and females did not identify any evident differences.

Table IV.

ROC indices of hepatic parameters for diagnosing or predicting IM.

Table IV.

ROC indices of hepatic parameters for diagnosing or predicting IM.

A, Male

ParametersAUCCut-off valueSensitivity (%)Specificity (%)Accuracy (%)PPV (%)NPV (%)
ALT0.764a27.5 U/l72.30076.60074.46875.55673.469
AST0.752a21.5 U/l70.20072.30071.27771.73970.833
GGT0.809a29.5 U/l74.50076.60075.53276.08775.000
TB0.676a10.70 µmol/l63.80066.00064.89464.58365.217

B, Female

ParametersAUCCut-off valueSensitivity (%)Specificity (%)Accuracy (%)PPV (%)NPV (%)

ALT0.698a15.5 U/l60.40075.00067.70870.73265.455
AST0.742a17.5 U/l64.60068.70066.66767.39166.000
GGT0.742a18.0 U/l68.80077.10072.91775.00071.154
TB0.759a7.45 µmol/l81.30075.00078.12580.00076.471

a P<0.01. ROC, receiver operating characteristic; IM, infectious mononucleosis; AUC, area under the curve; PPV, positive predictive value; NPV, negative predictive value; ALT, alanine aminotransferase; AST, aspartate aminotransferase; GGT, γ-glutamyl transferase; TB, total bilirubin.

Risks of IM in different hepatic function status

Binary logistic regression models were utilized to calculate the risks of IM in the males and females (Table V). For transferases, crude OR calculations were performed using the lowest quartiles as references. High transferase levels significantly increased IM risk, particularly in males. Following adjustments using age and BMI as covariates, males with high levels of transferases exhibited a much greater susceptibility to IM. Crude ORs in ALT quartile 4 were 21.667 and 10.111 for males and females, respectively; however adjusted ORs were 38.054 and 9.882, respectively (all P<0.01). For TB, crude OR calculations were performed using the highest quartile as a reference. Lower TB levels were deemed to significantly increase the risk of IM in males and females. Adjusted OR calculations used age and BMI as covariates. Notably, females exhibited a higher risk of developing IM when they exhibited low serum TB. Crude ORs in quartile 1 were 8.229 and 8.257 for males and females, respectively; however, adjusted ORs were 8.883 and 10.048, respectively (all P<0.01).

Table V.

IM risk according to liver parameter quartiles in the two sexes.

Table V.

IM risk according to liver parameter quartiles in the two sexes.

MalesFemales


Parameter valuesCrude OR (CI)cAdjusted OR (CI)dParameter valuesCrude OR (CI)cAdjusted OR (CI)d
ALT (U/l)
  ALT Quartile 1ALT≤15.00 (reference) ALT≤11.00 (reference)
  ALT Quartile 2 15.00<ALT≤25.001.238 (0.310–4.947)1.279 (0.306–5.341) 11.00<ALT≤14.000.674 (0.196–2.320)0.679 (0.196–2.351)
  ALT Quartile 3 25.00<ALT≤63.004.875 (1.230–19.319)a7.149 (1.635–31.265)b 14.00<ALT≤27.500.935 (0.299–2.920)0.933 (0.281–3.103)
  ALT Quartile 4ALT>63.0021.667 (4.154–113.020)b38.054 (6.241–232.038)bALT>27.5010.111 (2.306–44.348)b9.882 (2.076–47.038)b
AST (U/l)
  AST Quartile 1AST≤17.00 (reference) AST≤14.00 (reference)
  AST Quartile 2 17.00<AST≤21.001.813 (0.487–6.758)2.517 (0.639–9.909) 14.00<AST≤17.001.486 (0.386–5.722)1.462 (0.378–5.663)
  AST Quartile 3 21.00<AST≤38.254.636 (1.309–16.423)a6.948 (1.783–27.072)b17.00< AST≤26.001.878 (0.536–6.581)1.924 (0.545–6.793)
  AST Quartile 4AST>38.2516.150 (3.718–70.142)b26.313 (5.384–128.603)bAST>26.0019.067 (3.899–93.227)b20.642 (3.936–108.262)b
GGT (U/l)
  GGT Quartile 1GGT≤18.00 (reference) GGT≤10.00 (reference)
  GGT Quartile 2 18.00<GGT≤28.001.684 (0.427–6.642)3.074 (0.694–13.610) 10.00<GGT≤16.000.943 (0.253–3.509)0.977 (0.260–3.668)
  GGT Quartile 3 28.00<GGT≤63.256.667 (1.690–26.298)b20.922 (3.910–111.945)b 16.00<GGT≤34.753.000 (0.807–11.147)3.030 (0.797–11.513)
  GGT Quartile 4GGT>63.2526.667 (5.199–136.777)b79.281 (11.149–563.762)bGGT>34.758.360 (1.971–35.461)b9.093 (1.927–42.903)b
TB (µmol/l)
  TB Quartile 1TB≤8.2508.229 (2.175–31.132)b8.883 (2.287–34.502)bTB≤5.7008.257 (2.184–31.222)b10.048 (2.482–40.682)b
  TB Quartile 2 8.250<TB≤10.5505.040 (1.400–18.140)a5.220 (1.402–19.436)a 5.700<TB≤7.6009.229 (2.463–34.583)b10.457 (2.633–41.535)b
  TB Quartile 3 10.550<TB≤15.5753.600 (1.007–12.865)a3.839 (1.041–14.158)a 7.600<TB≤11.6500.578 (0.155–2.151)0.586 (0.153–2.249)
  TB Quartile 4TB>15.575 (reference) TB>11.650 (reference)

a P<0.05

b P<0.01.

c Logistic regression model without covariates

d Logistic regression model with age and BMI as covariates. IM, infectious mononucleosis; OR, odds ratio; CI, confidence interval; ALT, alanine aminotransferase; AST, aspartate aminotransferase; GGT, γ-glutamyl transferase; TB, total bilirubin.

Discussion

Worldwide, >95% of adults are infected with EBV and in industrialized countries, it is estimated that ~50% of the population experiences primary EBV infection <5 years old (1). Most people infected with EBV are either asymptomatic or exhibit mild symptoms; however, some will develop IM. Mild to moderate elevations of liver enzymes occur in 80–90% of patients with IM, however jaundice only occurs in 5% (13,13). Furthermore, hepatic failure in patients with IM has been reported only occasionally (1618). The three most clinically relevant transferases are ALT, AST and GGT, which are the primary indices of liver function (19). Transaminases transfer amino and keto groups between amino acids and keto acids (19). ALT catalyzes this interconversion between L-alanine and α-ketoglutarate on one side and pyruvate and L-glutamate on the other. AST catalyzes this interconversion between aspartate and α-ketoglutarate on one side and oxaloacetate and glutamate on the other (19). GGT belongs to the subclass of amino-acyltransferases, which transfer acyl groups between amino acids and peptides (19). GGT catalyzes the transfer from a 5-L-glutamyl-peptide and an amino acid on one side to a peptide and a 5-L-glutamyl amino acid on the other (19). Analysis of liver biopsies taken from patients with IM identified marked periportal lymphocytic infiltration, marked Kupffer cell activity and considerable acidophilic degeneration of individual liver cells (20), which may explain why transferase levels are increased in patients with IM. By contrast, patients with infectious hepatitis exhibit necrosis and inflammatory exudates (21). The results of the current study indicate that ALT, AST and GGT levels are significantly increased in males and females with IM compared with controls, indicating that transferase levels may be used to diagnose IM or determine IM risk. High transferase levels significantly increased the risk of IM, particularly in males.

Bilrubin is a by-product of heme degradation (22). The enzyme heme-oxygenase catalyzes heme and results in the formation of three products: Biliverdin, ferrous iron and carbon monoxide. Biliverdin is reduced to bilirubin by biliverdin reductase, bilirubin then binds to albumin and circulates in the blood. Bilirubin is then neutralized following its encounter with hepatocytes (22). The metabolic pathway that converts biliverdin, a soluble and nontoxic heme product, into bilirubin, which is insoluble and potentially toxic, has been evolutionarily conserved (22). It has therefore been hypothesized that bilirubin may serve an important physiological role. Previous studies have demonstrated that, although bilirubin is cytotoxic at high concentrations, at physiological concentrations bilirubin is able to scavenge free radicals and has powerful immunosuppressive effects (2325). Indeed, the antioxidant effect of bilirubin is ~20 times more potent than that of Vitamin E, a known scavenger of free radicals (26).

Low serum bilirubin was identified as a strong, independent risk factor for coronary disease in 1994 (27) and the inverse association between bilirubin levels and coronary artery disease has been confirmed (28,29). Targeting heme-oxygenase and regulating biliverdin reductase may be novel methods of treating coronary artery disease and are currently under investigation (28,29). Niacin induces heme-oxygenase, which may partially explain its vascular protective properties (30). Bilirubin affects the immune system at physiological concentrations (23); for example, bilirubin inhibits the complement cascade by interrupting the binding of the C1 complex to the complement antibody (31). Bilirubin may also inhibit the cell-surface expression of major histocompatibility complex II molecules on antigen-presenting cells (32). Furthermore, following entry into a target cell, bilirubin may engage in the widespread inhibition of protein kinases (33). In various inflammatory and immunological disorders, including multiple sclerosis (34), Crohn's disease (35) and systemic lupus erythematosus (36), bilirubin may be depleted following completion of its functions.

One of the objectives of the current study was to investigate the association between IM and bilirubin. It has been demonstrated that oxidative stress serves a crucial role in the pathogenesis of various infections, including the hepatitis virus (37), respiratory viruses (38), human immunodeficiency virus (39), influenza virus (40), Staphylococcus aureus (41), Helicobacter pylori (42) and mycoplasma (43). It is hypothesized that an infection due to EBV may also cause oxidative stress, leading to the marked depletion of antioxidants, such as bilirubin. This may be the underlying mechanism explaining the decrease in bilirubin observed in patients with IM in the current study. Furthermore, the present study indicates that females with low bilirubin levels are more susceptible to developing IM than males with low bilirubin levels. Males generally exhibit much higher serum bilirubin than females (44) and this was confirmed in the current study. Higher bilirubin levels may confer more antioxidant protection in males, therefore females may be more susceptible to the oxidative stress caused by bilirubin depletion. Consequently, decreased bilirubin may be more predictive of IM infection in females than males.

In the present study, there were differences between males and females among indices other than liver function parameters. Cr and BUN levels, as well as WBC levels, were significantly higher in males than in females. Previous studies have demonstrated that Cr levels are equal in children; however during adolescence, Cr levels increase more in males than females (45,46). In adulthood, females have lower serum Cr values than males with similar renal function (47). Cr and BUN induce the same effects in males and females (4853). The results of studies investigating WBC levels are inconsistent; certain studies indicate that WBC levels are higher in males (54,55), whereas others indicate that WBC levels are higher in females (56,57). However, major influential factors include age and sex hormone levels; indeed, it has been suggested that WBC levels may change in females at different stages of the menstrual cycle (54). In addition, liver function parameters are associated with age, body fat, renal function and blood cells (4853) and correlations between these key variables were identified in the current study.

There were several limitations of the current study. Firstly, the cross-sectional design of the current investigation meant that no causality could be determined. A prospective study should therefore be performed in the future. Secondly, a limited number of patients with IM and controls were evaluated and they were only recruited from a single center. More participants should be recruited from multiple centers in order to verify the results of the current study. Thirdly, due to budget limitations of the current study, measurements of serum parameters were based on a single determination and markers of inflammation or activities of antioxidants were not assessed. Finally, the administration of bilirubin as a possible adjuvant therapy should be investigated in the future to validate the results of the current study.

In conclusion, the results of the current study identified a positive association between transferases and IM and a negative association between TB and IM, suggesting that there is a progressive decrease of antioxidant reserves during IM. High transferase levels were suggestive of IM, particularly in males, whereas low TB was suggestive of IM, particularly in females.

Acknowledgements

The present study was supported by the National Key Clinical Specialty Project; the Tianjin Medical University General Hospital New Century Excellent Talent Program; the Young and Middle-aged Innovative Talent Training Program from Tianjin Education Committee; and the Talent Fostering Program (the 131 Project) from Tianjin Education Committee, Tianjin Human Resources and Social Security Bureau. The present study was also supported by China National Natural Science Foundation (grant no. 81571709), the Key Project of Tianjin Science and Technology Committee Foundation (grant no. 16JCZDJC34300); and the Tianjin Science and Technology Committee Foundation (grant nos. 11ZCGYSY05700, 12ZCZDSY20400 and 13ZCZDSY20200).

Glossary

Abbreviations

Abbreviations:

IM

infectious mononucleosis

EBV

Epstein-Barr virus

ALT

alanine aminotransferase

AST

aspartate aminotransferase

GGT

γ-glutamyl transferase

BH

body height

BW

body weight

BMI

body mass index

WBC

white blood cell

RBC

red blood cell

Hg

hemoglobin

PLT

platelet

TB

total bilirubin

BUN

blood urea nitrogen

Cr

creatinine

ROC

receiver operating characteristic

OR

odds ratio

CI

confidence interval

References

1 

Luzuriaga K and Sullivan JL: Infectious mononucleosis. N Engl J Med. 362:1993–2000. 2010. View Article : Google Scholar : PubMed/NCBI

2 

Vouloumanou EK, Rafailidis PI and Falagas ME: Current diagnosis and management of infectious mononucleosis. Curr Opin Hematol. 19:14–20. 2012. View Article : Google Scholar : PubMed/NCBI

3 

Wang Y, Li J, Ren YY and Zhao H: The levels of liver enzymes and atypical lymphocytes are higher in youth patients with infectious mononucleosis than in preschool children. Clin Mol Hepatol. 19:382–388. 2013. View Article : Google Scholar : PubMed/NCBI

4 

Topp SK, Rosenfeldt V, Vestergaard H, Christiansen CB and Von Linstow ML: Clinical characteristics and laboratory findings in Danish children hospitalized with primary Epstein-Barr virus infection. Infect Dis (Lond). 47:908–914. 2015. View Article : Google Scholar : PubMed/NCBI

5 

Cohn C and Lidman BI: Hepatitis without jaundice in infectious mononucleosis. J Clin Invest. 25:145–151. 1946. View Article : Google Scholar

6 

Rosalki SB, Jones TG and Verney AF: Transaminase and liver-function studies in infectious mononucleosis. Br Med J. 1:929–932. 1960. View Article : Google Scholar : PubMed/NCBI

7 

Horwitz CA, Burke MD, Grimes P and Tombers J: Hepatic function in mononucleosis induced by Epstein-Barr virus and cytomegalovirus. Clin Chem. 26:243–246. 1980.PubMed/NCBI

8 

Yang SI, Geong JH and Kim JY: Clinical characteristics of primary Epstein Barr virus hepatitis with elevation of alkaline phosphatase and γ-glutamyltransferase in children. Yonsei Med J. 55:107–112. 2014. View Article : Google Scholar : PubMed/NCBI

9 

Uluğ M, Celen MK, Ayaz C, Geyik MF and Hoşoğlu S: Acute hepatitis: A rare complication of Epstein-Barr virus (EBV) infection. J Infect Dev Ctries. 4:668–673. 2010. View Article : Google Scholar : PubMed/NCBI

10 

Zenda T, Itoh Y, Takayama Y, Masunaga T, Asaka S, Oiwake H, Shinozaki K and Takeda R: Significant liver injury with dual positive IgM antibody to Epstein-Barr virus and cytomegalovirus as a puzzling initial manifestation of infectious mononucleosis. Intern Med. 43:340–343. 2004. View Article : Google Scholar : PubMed/NCBI

11 

Baron DN, Bell JL and Dunnet WN: Biochemical studies on hepatic involvement in infectious mononucleosis. J Clin Pathol. 18:209–211. 1965. View Article : Google Scholar : PubMed/NCBI

12 

Evans AS: Liver involvement in infectious mononucleosis. J Clin Invest. 27:106–110. 1948. View Article : Google Scholar : PubMed/NCBI

13 

Straus SE, Cohen JI, Tosato G and Meier J: NIH conference. Epstein-Barr virus infections: Biology, pathogenesis, and management. Ann Intern Med. 118:45–58. 1993. View Article : Google Scholar : PubMed/NCBI

14 

Hu L, Yang J, Cui T, Xing H and Cai P: Diagnosis of infectious mononucleosis by combined detection of atypical lymphocytes and transaminase. J Huazhong Univ Sci Technolog Med Sci. 26:384–385. 2006. View Article : Google Scholar : PubMed/NCBI

15 

DeLong ER, DeLong DM and Clarke-Pearson DL: Comparing the areas under two or more correlated receiver operating characteristic curves: A nonparametric approach. Biometrics. 44:837–845. 1988. View Article : Google Scholar : PubMed/NCBI

16 

Ainley NJ: A fatal case of infectious mononucleosis with extensive zonal necrosis of the liver. Ulster Med J. 18:219–224. 1949.PubMed/NCBI

17 

Harries JT and Ferguson AW: Fatal infectious mononucleosis with liver failure in two sisters. Arch Dis Child. 43:480–485. 1968. View Article : Google Scholar : PubMed/NCBI

18 

McMahon JM, Elliott CW and Green RC: Infectious mononucleosis complicated by hepatic coma. Am J Gastroenterol. 51:200–207. 1969.PubMed/NCBI

19 

Herbinger KH, Hanus I, Felbinger TW, Weber C, Beissner M, von Sonnenburg F, Löscher T, Bretzel G, Nothdurft HD, Hoelscher M and Alberer M: Elevated values of clinically relevant transferases induced by imported infectious diseases: A controlled cross-sectional study of 14,559 diseased german travelers returning from the tropics and subtropics. Am J Trop Med Hyg. 95:481–487. 2016. View Article : Google Scholar : PubMed/NCBI

20 

Wadsworth RC and Keil PG: Biopsy of the liver in infectious mononucleosis. Am J Pathol. 28:1003–1025. 1952.PubMed/NCBI

21 

Sullivan BH Jr, Irey NS, Pileggi VJ, Crone RI and Gibson JR: The liver in infectious mononucleosis. Am J Dig Dis. 2:210–223. 1957. View Article : Google Scholar : PubMed/NCBI

22 

Maines MD: The heme oxygenase system: A regulator of second messenger gases. Annu Rev Pharmacol Toxicol. 37:517–554. 1997. View Article : Google Scholar : PubMed/NCBI

23 

Jangi S, Otterbein L and Robson S: The molecular basis for the immunomodulatory activities of unconjugated bilirubin. Int J Biochem Cell Biol. 45:2843–2851. 2013. View Article : Google Scholar : PubMed/NCBI

24 

Kapitulnik J: Bilirubin: An endogenous product of heme degradation with both cytotoxic and cytoprotective properties. Mol Pharmacol. 66:773–779. 2004. View Article : Google Scholar : PubMed/NCBI

25 

Stocker R, Yamamoto Y, McDonagh AF, Glazer AN and Ames BN: Bilirubin is an antioxidant of possible physiological importance. Science. 235:1043–1046. 1987. View Article : Google Scholar : PubMed/NCBI

26 

Wu TW, Fung KP and Yang CC: Unconjugated bilirubin inhibits the oxidation of human low density lipoprotein better than Trolox. Life Sci. 54:P477–P481. 1994. View Article : Google Scholar : PubMed/NCBI

27 

Schwertner HA, Jackson WG and Tolan G: Association of low serum concentration of bilirubin with increased risk of coronary artery disease. Clin Chem. 40:18–23. 1994.PubMed/NCBI

28 

Akboga MK, Canpolat U, Sahinarslan A, Alsancak Y, Nurkoc S, Aras D, Aydogdu S and Abaci A: Association of serum total bilirubin level with severity of coronary atherosclerosis is linked to systemic inflammation. Atherosclerosis. 240:110–114. 2015. View Article : Google Scholar : PubMed/NCBI

29 

Ghem C, Sarmento-Leite RE, de Quadros AS, Rossetto S and Gottschall CA: Serum bilirubin concentration in patients with an established coronary artery disease. Int Heart J. 51:86–91. 2010. View Article : Google Scholar : PubMed/NCBI

30 

Wu BJ, Chen K, Barter PJ and Rye KA: Niacin inhibits vascular inflammation via the induction of heme oxygenase-1. Circulation. 125:150–158. 2012. View Article : Google Scholar : PubMed/NCBI

31 

Basiglio CL, Arriaga SM, Pelusa HF, Almará AM, Roma MG and Mottino AD: Protective role of unconjugated bilirubin on complement-mediated hepatocytolysis. Biochim Biophys Acta. 1770:1003–1010. 2007. View Article : Google Scholar : PubMed/NCBI

32 

Wu J, Ma J, Fan ST, Schlitt HJ and Tsui TY: Bilirubin derived from heme degradation suppresses MHC class II expression in endothelial cells. Biochem Biophys Res Commun. 338:890–896. 2005. View Article : Google Scholar : PubMed/NCBI

33 

Hansen TW, Mathiesen SB and Walaas SI: Bilirubin has widespread inhibitory effects on protein phosphorylation. Pediatr Res. 39:1072–1077. 1996. View Article : Google Scholar : PubMed/NCBI

34 

Peng F, Deng X, Yu Y, Chen X, Shen L, Zhong X, Qiu W, Jiang Y, Zhang J and Hu X: Serum bilirubin concentrations and multiple sclerosis. J Clin Neurosci. 18:1355–1359. 2011. View Article : Google Scholar : PubMed/NCBI

35 

Liechti FD, Grandgirard D and Leib SL: Bacterial meningitis: Insights into pathogenesis and evaluation of new treatment options: A perspective from experimental studies. Future Microbiol. 10:1195–1213. 2015. View Article : Google Scholar : PubMed/NCBI

36 

Vitek L, Muchová L, Jančová E, Pešičková S, Tegzová D, Peterová V, Pavelka K, Tesař V and Schwertner H: Association of systemic lupus erythematosus with low serum bilirubin levels. Scand J Rheumatol. 39:480–484. 2010. View Article : Google Scholar : PubMed/NCBI

37 

Zuwała-Jagiełło J, Warwas M and Pazgan-Simon M: Ischemia-modified albumin (IMA) is increased in patients with chronic hepatitis C infection and related to markers of oxidative stress and inflammation. Acta Biochim Pol. 59:661–667. 2012.PubMed/NCBI

38 

Komaravelli N and Casola A: Respiratory viral infections and subversion of cellular antioxidant defenses. J Pharmacogenomics Pharmacoproteomics. 5:pii: 10001412014.

39 

Ngondi JL, Oben J, Forkah DM, Etame LH and Mbanya D: The effect of different combination therapies on oxidative stress markers in HIV infected patients in Cameroon. AIDS Res Ther. 3:192006. View Article : Google Scholar : PubMed/NCBI

40 

Checconi P, Salzano S, Bowler L, Mullen L, Mengozzi M, Hanschmann EM, Lillig CH, Sgarbanti R, Panella S, Nencioni L, et al: Redox proteomics of the inflammatory secretome identifies a common set of redoxins and other glutathionylated proteins released in inflammation, influenza virus infection and oxidative stress. PLoS One. 10:e01270862015. View Article : Google Scholar : PubMed/NCBI

41 

Chakraborty SP, Das S, Chattopadhyay S, Tripathy S, Dash SK, Pramanik P and Roy S: Staphylococcus aureus infection induced redox signaling and DNA fragmentation in T-lymphocytes: Possible ameliorative role of nanoconjugated vancomycin. Toxicol Mech Methods. 22:193–204. 2012. View Article : Google Scholar : PubMed/NCBI

42 

Aslan M, Nazligul Y, Horoz M, Bolukbas C, Bolukbas FF, Aksoy N, Celik H and Erel O: Serum prolidase activity and oxidative status in Helicobacter pylori infection. Clin Biochem. 40:37–40. 2007. View Article : Google Scholar : PubMed/NCBI

43 

Kariya C, Chu HW, Huang J, Leitner H, Martin RJ and Day BJ: Mycoplasma pneumoniae infection and environmental tobacco smoke inhibit lung glutathione adaptive responses and increase oxidative stress. Infect Immun. 76:4455–4462. 2008. View Article : Google Scholar : PubMed/NCBI

44 

Rosenthal P, Pincus M and Fink D: Sex- and age-related differences in bilirubin concentrations in serum. Clin Chem. 30:1380–1382. 1984.PubMed/NCBI

45 

Pottel H, Vrydags N, Mahieu B, Vandewynckele E, Croes K and Martens F: Establishing age/sex related serum creatinine reference intervals from hospital laboratory data based on different statistical methods. Clin Chim Acta. 396:49–55. 2008. View Article : Google Scholar : PubMed/NCBI

46 

Uemura O, Honda M, Matsuyama T, Ishikura K, Hataya H, Yata N, Nagai T, Ikezumi Y, Fujita N, Ito S, et al: Age, gender, and body length effects on reference serum creatinine levels determined by an enzymatic method in Japanese children: A multicenter study. Clin Exp Nephrol. 15:694–699. 2011. View Article : Google Scholar : PubMed/NCBI

47 

O'Leary JG, Wong F, Reddy KR, Garcia-Tsao G, Kamath PS, Biggins SW, Fallon MB, Subramanian RM, Maliakkal B, Thacker L and Bajaj JS: Gender-specific differences in baseline, peak, and delta serum creatinine: The NACSELD experience. Dig Dis Sci. 62:768–776. 2017. View Article : Google Scholar : PubMed/NCBI

48 

Meng Z, Liu M, Zhang Q, Liu L, Song K, Tan J, Jia Q, Zhang G, Wang R, He Y, et al: Gender and age impacts on the association between thyroid function and metabolic syndrome in Chinese. Medicine (Baltimore). 94:e21932015. View Article : Google Scholar : PubMed/NCBI

49 

Meng Z, Liu M, Zhang Q, Liu L, Song K, Tan J, Jia Q, Zhang G, Wang R, He Y, et al: Gender and age impact on the association between thyroid-stimulating hormone and serum lipids. Medicine (Baltimore). 94:e21862015. View Article : Google Scholar : PubMed/NCBI

50 

Ren X, Meng Z, Liu M, Zhu M, He Q, Zhang Q, Liu L, Song K, Jia Q, Jia Q, et al: No associations exist between mean platelet volume or platelet distribution width and thyroid function in Chinese. Medicine (Baltimore). 95:e45732016. View Article : Google Scholar : PubMed/NCBI

51 

Zhou P, Meng Z, Liu M, Ren X, Zhu M, He Q, Zhang Q, Liu L, Song K, Jia Q, et al: The associations between leukocyte, erythrocyte or platelet, and metabolic syndrome in different genders of Chinese. Medicine (Baltimore). 95:e51892016. View Article : Google Scholar : PubMed/NCBI

52 

Wang S, Zhang J, Zhu L, Song L, Meng Z, Jia Q, Li X, Liu N, Hu T, Zhou P, et al: Association between liver function and metabolic syndrome in Chinese men and women. Sci Rep. 7:448442017. View Article : Google Scholar : PubMed/NCBI

53 

Zhang J, Meng Z, Zhang Q, Liu L, Song K, Tan J, Li X, Jia Q, Zhang G and He Y: Gender impact on the correlations between subclinical thyroid dysfunction and hyperuricemia in Chinese. Clin Rheumatol. 35:143–149. 2016. View Article : Google Scholar : PubMed/NCBI

54 

Nowak J, Borkowska B and Pawlowski B: Leukocyte changes across menstruation, ovulation, and mid-luteal phase and association with sex hormone variation. Am J Hum Biol. 28:721–728. 2016. View Article : Google Scholar : PubMed/NCBI

55 

Pérez-de-Heredia F, Gómez-Martínez S, Díaz LE, Veses AM, Nova E, Wärnberg J, Huybrechts I, Vyncke K, Androutsos O, Ferrari M, et al: Influence of sex, age, pubertal maturation and body mass index on circulating white blood cell counts in healthy European adolescents-the HELENA study. Eur J Pediatr. 174:999–1014. 2015. View Article : Google Scholar : PubMed/NCBI

56 

Mandala WL, Gondwe EN, MacLennan JM, Molyneux ME and MacLennan CA: Age- and sex-related changes in hematological parameters in healthy Malawians. J Blood Med. 8:123–130. 2017. View Article : Google Scholar : PubMed/NCBI

57 

Pratley RE, Wilson C and Bogardus C: Relation of the white blood cell count to obesity and insulin resistance: Effect of race and gender. Obes Res. 3:563–571. 1995. View Article : Google Scholar : PubMed/NCBI

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Spandidos Publications style
Zhang L, Zhou P, Meng Z, Pang C, Gong L, Zhang Q, Jia Q and Song K: Infectious mononucleosis and hepatic function. Exp Ther Med 15: 2901-2909, 2018
APA
Zhang, L., Zhou, P., Meng, Z., Pang, C., Gong, L., Zhang, Q. ... Song, K. (2018). Infectious mononucleosis and hepatic function. Experimental and Therapeutic Medicine, 15, 2901-2909. https://doi.org/10.3892/etm.2018.5736
MLA
Zhang, L., Zhou, P., Meng, Z., Pang, C., Gong, L., Zhang, Q., Jia, Q., Song, K."Infectious mononucleosis and hepatic function". Experimental and Therapeutic Medicine 15.3 (2018): 2901-2909.
Chicago
Zhang, L., Zhou, P., Meng, Z., Pang, C., Gong, L., Zhang, Q., Jia, Q., Song, K."Infectious mononucleosis and hepatic function". Experimental and Therapeutic Medicine 15, no. 3 (2018): 2901-2909. https://doi.org/10.3892/etm.2018.5736