Ginsenoside Rg1 attenuates liver injury induced by D‑galactose in mice
- Authors:
- Published online on: September 11, 2018 https://doi.org/10.3892/etm.2018.6727
- Pages: 4100-4106
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Copyright: © Xiao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Abstract
Introduction
Liver serves a crucial role in the metabolism of xenobiotics which may cause numerous hepatic diseases (1). A previous study has indicated that oxidative stress is a key element to the pathophysiology of various types of liver damage, including alcoholic and nonalcoholic liver disease (2), Previous studies have also confirmed that the reactive oxygen species (ROS) is a crucially important factor for the initiation of oxidative stress and that advanced glycation end products (AGEs) are associated with enhanced oxidative stress (3,4). D-galactose is a common oxidative stress model inducer and the tissues of animals with chronic D-gal administration induce oxidative damage and AGE formation (5). Oxidative stress causes the increase of liver cell membrane permeability, releasing ALT and AST into the blood, making these parameters suitable biochemical markers of liver damage (6). Furthermore, other biomarkers including superoxide dismutase (SOD), 8-hydroxy-2 deoxyguanosine (8-OH-dG), malondialdehyde (MDA), glutathione (GSH), glutathione peroxidase (GSH-Px), may be used to assess oxidative stress in liver disease (7).
Traditional treatment methods usually include administration of nucleoside analogues, interferon and antiviral drugs (8). Synthetic drugs for the treatment of liver diseases can lead to severe side-effects (9), and, therefore, herbal medication may be an alternative treatment method. There is a long history of using traditional Chinese medicines in treatment of liver diseases (10). Ginsenoside Rg1 (Rg 1) is one of the most active ingredients of ginseng which induces antiaging and antioxidative effects, as well as improves immunity and memory (11,12). Previous studies have investigated the effect of Rg1 on liver damage and demonstrated that this compound reduces apoptosis, and inflammatory response to protect the liver from ischemia reperfusion injury and type 2 diabetes (13,14). Rg1 also enhanced the antioxidative defense system to ameliorate carbon tetrachloride-induced liver injury in rats (15). The present study used a mouse model of D-gal-induced liver injury to elucidate the function and the underlying mechanism of Rg1.
Materials and methods
Animals
SPF male C57BL/6J mice (weight, 16±2 g; n=30; age, 6–8 weeks) were purchased from the Medical and Laboratory Animal Center of Chongqing University (Chongqing, China) and housed at a temperature of 22–24°C, a humidity of 40–70% and a 12 h light/dark cycle, with free access to water and food. All animal experiments were performed in accordance with the institutional and national guidelines and regulations, and approved by the Chongqing Medical University Animal Care and Use Committee.
Reagents
Rg1 (purity >95%) was purchased from Hongjiu Biotech Co., Ltd., (Tonghua, China). D-galactose (D-gal; purity >99%) was obtained from Sangon Biotech Co., Ltd. (Shanghai, China). The senescence-associated β-galactosidase (SA-β-gal) Staining kit was purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA), whereas the SOD, GSH-Px, GSH and MDA detection kits were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). The AGEs (cat. no. abx512406) and 8-OH-dG (cat. no. SKT-120-480) kits were purchased from Shanghai Yuanye Biological Technology Co., Ltd. (Shanghai, China).
Mouse D-gal aging model and treatment groups
A total of 30 mice were randomly divided into three groups: Control group, D-gal-administration group (D-gal group) and D-gal-administration plus Rg1 treatment group (D-gal + Rg1 group). In the D-gal group, D-gal (120 mg/kg body weigh/day) was injected intraperitoneally into mice for 42 days. In the D-gal + Rg1 group, 120 mg/kg body weigh/day D-gal was also administered to mice intraperitoneally and Rg1 (20 mg/kg body weight/day) was concomitantly injected for 35 days from the 8th day of the D-gal injection. All control animals were given an equal volume of saline intraperitoneally. On the 43rd day of the experiment, the animals were weighted (27.20±1.37 g) and anesthetized by intraperitoneal injection of sodium pentobarbital at a dose of 40 mg/kg. The blood was collected from the retro-orbital plexus (0.3 ml per mice) and liver samples were dissected and weighted immediately. The anesthetized mice were sacrificed by blood loss from heart puncture (0.6±0.1 ml per mice) until the mice succumbed to mortality confirmed by muscle relaxation and no respiratory circulation. The blood and animal carcass were collected and returned to Medical and Laboratory Animal Center of Chongqing for safe disposal.
Detection of hepatic index
The hepatic indices of each group were calculated using the following equation: Hepatic index=liver weight (mg)/body weight (g).
Biochemical determination
The collected blood was allowed to clot and serum was separated by centrifugation at a speed of 1,200 × g for 15 min at 4°C. Serum biochemical parameters of liver function including alanine aminotransferase (ALT), aminotransferase (AST), albumin (Alb) and total bilirubin (TBiL) were analyzed by the Department of Laboratory Medicine of the First Affiliated Hospital Group of Chongqing Medical University (Chongqing, China).
Histological examination
For histological studies, the liver tissues were fixed with 4% paraformaldehyde at room temperature for 24 h, dehydrated in graded (50–100%) alcohol series and embedded in paraffin. Thin sections (5 µm) were cut and placed into a glass dish with hematoxylin. Following agitation for 30 sec at room temperature, slides were washed using water for 1 min. Slides were then stained with 1% eosin for 30 sec at room temperature. Sections were then dehydrated using alcohol and washed with xylene. One or two drops of mounting medium was then placed onto samples and covered with a coverslip. Samples were observed using bright field microscopy (magnification, ×200) to observe sections. The initial examination was qualitative, with the purpose of determining histopathological lesions of the liver tissue. For transmission electron microscopy (TEM) liver tissues were fixed with 2.5% buffered glutaraldehyde for 4 h at 4°C. Tissues were subsequently washed three times for 15 min with PBS. Tissues were then fixed with 1% osmium for 1 h at 4°C and dehydrated with different concentrations of alcohol (50, 70, 80, 90 and 95%) for 15 min each. then infiltration and embedding tissues at 70°C overnight. Tissues were then sliced into 100 nm sections and stained with 0.5% toluidine blue for 30 min at 60°C. Samples were observed using TEM.
SA-β-gal cytochemical staining
The SA-β-gal staining was carried out according to the manufacturer's protocol of the SA-β-gal Staining kit. The preparation of liver tissue paraffin sections (5 µm) was performed as aforementioned. Briefly, slides were washed twice for >5 min using PBS, fixed with the Fixative Solution for 15 min at room temperature and stained by X-Gal Staining Solution (100 mM sodiumphosphate, 2 mM MgCl2, 150 mM sodium chloride, 0.01% sodium deoxycholate, 0.02% NP-40, 5 mM potassium ferricyanide, 5 mM potassium ferrocyanide and 1 mg/ml X-gal; pH 6) for 24 h at 37°C without CO2. After the incubation, sections were washed in PBS, stained with eosin for 30 sec at room temperature and viewed using bright field microscopy (magnification, ×200). Quantitative image analysis included at least 3 random fields of view per slide and was performed in a blinded manner. The intensity of SA-β-gal-positive staining was evaluated using the relative optical density (ROD) value. ROD of SA-β-gal-positive cells in the liver was obtained following transformation of mean gray values into ROD using the following formula: ROD=log (256/mean gray value) (16). Images were captured from five randomly selected fields of view using Image Pro Plus software 6.0 (Media Cybernetics, Inc., Rockville, MD, USA).
Detection of oxidation-associated biomarkers
The liver was collected and lysed in a −4°C ice bath for 30 min. The supernatant was collected after centrifugation at 12,000 r/min and 4°C for 15 min. GSH-Px and SOD activity, and GSH and MDA contents were detected by colorimetric analysis according to the manufacturer's protocol.
Detection of AGEs and 8-OH-dG in the liver homogenate by ELISA
The supernatant was collected as described above, and the levels of AGEs and 8-OH-dG in the liver in each group were measured by ELISA kits according to the manufacturer's protocol.
Statistical analysis
Data were analyzed using one-way analysis of variance followed by a post-hoc Tukey's test for multiple comparisons. SPSS software (version 17.0; SPSS, Inc., Chicago, IL, USA) was used to analyze data, which are presented as the mean ± standard deviation. P<0.05 was considered to indicate a statistically significant difference.
Results
Effect of Rg1 on the hepatic index in a mouse model of hepatic injury
Hepatic indices in the D-gal group were significantly higher compared with the control and the D-gal + Rg1 groups (P<0.05; Table I).
Effect of Rg1 on the liver damage indexes
Administration of D-gal significantly altered the biochemical parameters compared with the control group (all P<0.05; Table II). Treatment with Rg1 significantly decreased the serum levels of AST, ALT and TBiL (all P<0.05) and elevated the serum level of Alb (P<0.05) compared with the D-gal group.
Effect of Rg1 on liver histopathology
The liver tissue structure in the three groups was assessed by optical microscopy (Fig. 1). The mice in the control group exhibited normal and intact lobular architecture and structure of hepatocytes. Histological examination of livers exposed to D-gal indicated severe liver damage, including dilated liver sinusoids, disordered arrangement of hepatocytes, multiple and extensive areas of portal inflammation and hepatocellular necrosis, as well as an increasing number of adipose cell. However, these pathological alterations were attenuated in animals treated with Rg1. TEM was also used to compare the three groups (Fig. 2). Numerous free ribosomes were identified in the cytoplasm of the control group hepatocytes and the cytoplasmic organelles included mitochondria, rough endoplasmic reticulum (RER) and normal and activated lysosomes. Following administration with D-gal for 42 days, dense deposits in the cytoplasm of hepatocytes were observed. An increased number of vacuoles, mitochondrial swelling and shortened, disrupted RER were observed in the cytoplasm. Clumping of nuclear chromatin was observed. However, the damage of hepatocytes was attenuated following treatment with Rg1.
Antioxidative effect of Rg1 in the mouse model of hepatic injury
Oxidative stress caused by ROS is one of the main causes of cell injury (17). SOD and GSH-Px are enzymes that participate in the removal of ROS from the cellular environment (18,19). MDA is an end-product of ROS-induced peroxidation and it is widely used as an oxidative stress biomarker (20); while GSH is the substrate of GSH-Px. Compared with the control group, SOD and GSH-Px activities, and GSH content decreased significantly in the D-group liver while the MDA content increased (all P<0.05; Table III). By contrast, Rg1 rescued the SOD and GSH-Px activities, lowered the GSH consumption significantly, and partially reversed the increase in MDA compared with the D-gal group (Table III).
Rg1 reduced the SA-β-gal staining in the liver
Asymmetric ROS are molecules containing unpaired, highly reactive electrons, leading to cell injury and aging (21). SA-β-gal is one of commonly used biomarkers of cell senescence (22), and, therefore, in the present study the cytoplasm of ageing cells stained blue (Fig. 3; arrows). Few SA-β-gal-positive cells were observed in the control group (Fig. 3A). The intensity of SA-β-gal staining was evaluated using the ROD value. D-gal administration induced a significant increase in the ROD value of the SA-β-gal staining, compared with the control group (Figs. 3 and 4). However, in the D-gal + Rg1 group, the ROD value significantly decreased compared with the D-gal group (Figs. 3 and 4).
Effect of Rg1 on the DNA damage of hepatocytes
The oxidation of guanine to form 8-OH-dG is a marker of oxidative DNA damage (23). Based on the levels of 8-OH-dG, oxidative DNA damage was elevated in the D-gal group compared with the control group. The level of 8-OH-dG in mice treated with Rg1 remained stable at a level of 44.49±4.20 ng/ml, and was significantly reduced compared with the D-gal group (Table IV).
Treatment with Rg1 decreased the levels of AGEs in hepatocytes from the mouse model of liver injury
AGEs are a group of modified molecular species formed by non-enzymatic reactions of reducing sugars and proteins, lipids or nucleic acids (24). The accumulation of AGEs is associated with hepatocyte damage and the process of aging (25). The administration of D-gal increased the liver accumulation of AGEs. However, treatment with Rg1 decreased the levels of AGEs compared with the D-gal model (Table IV).
Discussion
D-gal has been previously used to establish an experimental model of aging (26). A previous study has demonstrated that the primary advantage of long-term D-gal administration to mice is that it mimics the natural aging effect of the liver (27). The underlying mechanism of D-gal-induced injury is a decrease in the cellular UTP concentration leading to the inhibition of RNA and protein synthesis (28,29). Furthermore, oxidative damage and inflammation have also been hypothesized to serve roles in age-associated alterations in the liver (30). Hepatic aging and necrosis induced by the excessive levels of D-gal are associated with increased expression levels of oxygen-derived free radicals in mouse hepatocytes (31). Based on the results of previous studies, the present study established a D-gal-induced model to further investigate hepatic injury. In the present study, liver injury was induced by administration of D-gal and confirmed by alterations in liver damage indices, oxidative stress, histopathology, DNA damage, and levels of AGEs. All D-gal-induced alteration in the liver were significantly attenuated by administration of Rg1.
Liver damage induced by D-gal disrupts liver cell metabolism which leads to characteristic alterations in serum enzyme activity (32). Alterations in the membrane permeability of liver cells indicate the severity of hepatocellular damage induced by D-gal (6). In the present study, serum ALT, AST and TBiL activities increased following treatment with D-gal and significantly decreased following administration of Rg1. Administration of Rg1 increased the level of Alb in serum which decreased following treatment with D-gal. Furthermore, hepatic indices in the D-gal + Rg1 group were reduced compared with the D-gal group. The above results indicate that Rg1 may attenuate liver injury induced by D-gal in mice and therefore serve a protective role in liver damage.
Histological examination is necessary for determination of drug-induced liver protection. Tissues and cells are subjected to oxidative injury when large quantities of free radicals are generated or the activity of the antioxidant system deteriorates. The normal lobular architecture and structure of hepatic cells in the control group was altered in the D-gal group as indicated by hepatocellular necrosis and cytoplasm vacuolar degeneration. An improved architecture of the liver tissue was observed following administration of Rg1. TEM results revealed large numbers of free ribosomes in hepatocytes of the control group; cytoplasmic organelles were present and included mitochondria, RER and normal lysosomes. Following the administration of D-gal, there were numerous electron-dense deposits throughout hepatocyte cytoplasm and cytoplasmic organelles exhibited degenerative signs, including mitochondrial edema, shortened and disrupted RER, or vacuolation. Clumping of nuclear chromatin was observed. The D-gal-induced hepatic injury was attenuated by the administration of Rg1. Therefore, in the present study, administration of Rg1 served a protective role against the development of hepatic necrosis.
D-gal contributes to increased oxidative stress and formation of reactive oxygen species, which may result in hepatocyte damage or death (33). Free radicals may damage a number of cellular components including DNA, proteins and lipids (34,35). Antioxidation serves a protective role against D-gal-induced liver injury. SOD, which reduces the levels of superoxide anions and aids in the synthesis of hydrogen peroxide, is widely distributed in cells and protects from oxidative damage (36). GSH-Px catalyzes the reduction of hydrogen peroxide and lipid peroxide to non-toxic products and scavenges highly reactive lipid peroxides in the aqueous phase of the cell membrane (37). SOD and GSH-Px are important enzymes that participate in the removal of ROS from the cellular environment. GSH, an endogenous antioxidant, exhibits a particularly high concentration in the liver and is known for its key function as an electron donor during ROS metabolism and scavenge (38). Furthermore, GSH, GSH-Px and SOD decrease the levels of ROS to prevent the oxidation of proteins, lipids and DNA (39). MDA is an end-product of ROS-induced peroxidation, and it is commonly used as an oxidative stress biomarker (40). 8-OH-dG, a product of DNA oxidation, is a marker of oxidative DNA damage (41). The activity of SOD and GSH-PX, and the contents of GSH, MDA and 8-OH-dG may be used to measure the level of oxidative stress in cells (42), and to evaluate the protective effect of Rg1. In the present study, Rg1 increased the activity of SOD and GSH-Px and the contents of GSH compared with the D-gal group (43). Furthermore, treatment with Rg1 reduced the increase in the expression levels of MDA and 8-OH-dG caused by injury (44). These results indicated that Rg1 may act by enhancing the activity of endogenous antioxidative enzymes in cells and reducing the levels of peroxidation products.
Cell senescence is due to cellular damage caused by ROS (45). It has been reported that D-gal causes hepatocyte damage, induces cellular senescence and results in hepatic injury (46,47). Lysosomal dysfunction results in accumulation of SA-β-gal in aging cells, and, therefore, SA-β-gal is one of the most widely used biomarkers of aging (22). Furthermore, determination of cellular accumulation of AGEs may also be used to detect cellular aging (48). Both SA-β-gal and AGEs are common biomarkers used to evaluate senescence (49,50). The present study indicated that Rg1 inhibited cellular aging, which supports the results of a previous study where Rg1 alleviated senescence of hematopoietic stem/progenitor cells (51). Treatment with D-gal markedly increased the ROD value of SA-β-gal-positive cells and increased the accumulation of AGEs compared with the control group. D-gal-induced alterations were attenuated following treatment with Rg1. These results of the present suggest that Rg1 may alleviate the effects of D-gal on liver aging, and the underlying mechanism may be associated with the reduction of oxidative stress injury in cells.
In conclusion, the present study indicated that Rg1 improved the resistance of hepatic cells to D-gal-induced subacute liver injury in an in vivo mouse model. The results indicated that Rg1 exerted protective effects through its antioxidative properties, alleviation of DNA damage induced by chronic oxidative stress and enhanced activity of endogenous antioxidative defense enzymes. The present study may provide a theoretical and experimental basis for the application of Rg1 in the treatment of liver injury.
Acknowledgements
Not applicable.
Funding
The present study was supported by the National Natural Science Foundation of China (grant no. 81673748).
Availability of data and materials
The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
Authors' contributions
MHX, JYX and YPW conceived and designed the experiments of the current study. Performed the experiments: JYX, MHX, ZWL, WXH, YLF, DYJ, JL, PWJ and LW performed the experiments. JYX, MHX, JL and LW analyzed the data. WL, WXH and YLF provided reagents, materials and analysis tools. MHX and JX wrote the manuscript.
Ethics approval and consent to participate
All animal experiments were performed in accordance with the institutional and national guidelines and regulations, and approved by the Chongqing Medical University Animal Care and Use Committee.
Patient consent for publication
Not applicable.
Competing interests
The authors declare that they have no competing interests.
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