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Influence of neonatal gender on cord blood CD34+ cell amplification and gene expression
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- Published online on: May 7, 2019 https://doi.org/10.3892/etm.2019.7549
- Pages: 105-118
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Copyright: © Zhou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Abstract
Introduction
Hematopoietic stem cells (HSCs) are a specific type of cell with multi-potency and self-renewal ability (1). It has been demonstrated that bone marrow-reconstituting HSCs reside within a subpopulation of bone marrow or blood-derived mononuclear cells of a few percents which express the surface antigen CD34 (2). CD34 and CD38 surface antigens have long been used as surface molecules for the identification of primitive hematopoietic stem and progenitor cells. It has been reported that Severe combined immunodeficient-repopulating cells were present exclusively in the CD34+CD38− cell fraction (3,4). Under the same conditions, recipients of cord blood (CB) transplants experience a lower incidence of acute and chronic graft-vs.-host disease than recipients of bone marrow transplants (5). However, widespread utilization of CB is limited by the relatively low number of HSCs per unit, and most CB units provide insufficient stem cells for adults (6). Multiple strategies for overcoming these limitations are now being investigated. One important strategy involves ex vivo expansion of the umbilical CB unit prior to transplantation (7). However, the selection of the best source of HSCs remains the first and most important challenge.
To date, the nucleated cell dose has been used to select more suitable CB units for transplantation for a given patient. However, Aroviita et al (8) reported that female infants have higher median nucleated cell concentrations than male infants (13.9 vs. 13.3×109/l) after investigating 1,999 units of CB. By contrast, male infants had significantly higher median CD34+ cell concentrations than female infants (31.8 vs. 30.2/µl), even after correcting for birth weight. Bijou et al (9) also reported that male neonatal CB was significantly richer in CD34+ cells than female CB. From the above studies, it may be speculated that a correlation exists between neonatal gender and HSCs derived from CB. Therefore, in the present study, differences in the expansion of CD34+ cells from male and female neonatal CB and their gene expression were compared, and the underlying molecular mechanisms were explored. The detailed experimental design of the study is provided in the flow chart in Fig. 1.
Materials and methods
Collection and purification of CB CD34+ cells
Human CB samples (n=43; 20 males and 23 females) were obtained from mothers undergoing full-term deliveries between January and May 2015 at the Department of Obstetrics at Qilu Hospital of Shandong University (Jinan, China) after informed written consent was obtained. The maternal age was between 20 and 40 years (mean, 26±2 years), and there was no history of acute, chronic or infectious disease, neonatal apnea, edema or jaundice. The use of CB was approved by the Ethics Committee of Shandong University Qilu Hospital (Jinan, China). The birth weight was 3,000-4,000 g for the males and females considered for inclusion. Among the CB units harvested, only those in which at least 70 ml CB was collected and the total nucleated cell count exceeded 8×108 per unit were considered for processing.
Mononuclear cells (MNCs) of each single CB unit were separated using Ficollpaque medium (density, 1.077±0.001 g/ml; HaoYang Co.) and centrifuged at 1,726 × g at 12°C for 25 min. CB MNCs were incubated with 100 µl CD34+ micro beads (Miltenyi Biotec GmbH) at 4°C for 30 min. Cells were subsequently passed through an LS MACS column (Miltenyi Biotec GmbH) and enriched CD34+ cells were collected by flushing the column. Cells were subsequently suspended in 0.1 M PBS (pH 7.4). The purity of the CD34+ cells was detected by a fluorescence-assisted cell sorting (FACS) system (Guava easyCyte 6HT; EMD Millipore) and data were analyzed using Guava Incyte (version 2. 8; EMD Millipore).
CB CD34+ colony formation assay
CB CD34+ cells derived from male and female newborns were divided into two groups. CD34+ cells were seeded into 24-well plates for culture with semisolid medium (MethoCult GF H4434; Stem Cell Technologies) at a density of 1.0×104 cells/well according to the manufacturer's protocols. Cultures were maintained at 37°C in a humidified atmosphere of 5% CO2 and 20% O2 (HF240; Heal Force Tris-gas Incubator; Heal Force). After 2 weeks of culture, the numbers CFUs were determined under an inverted microscope (IX71; Olympus).
Amplification of CD34+ cells
CB CD34+ cells from CB from neonates of different gender were cultured in HSC expansion medium (Stem Cell Technologies, Inc.) containing 10% FBS (Gibco; Thermo Fisher Scientific, Inc.), 70 ng/ml stem cell factor, 30 ng/ml interleukin (IL)-3, 30 ng/ml FMS-like tyrosine kinase 3 ligand, 20 ng/ml IL-6, 20 ng/ml bone morphogenetic protein-2 and 20 ng/ml thrombopoietin at 1.1×105 cells/ml (R&D Systems, Inc.). Cytokine concentrations were determined as described previously (10,11). The medium was changed every 3–4 days. On day 7, the total suspension of cells was harvested for further analysis.
Immunophenotypic analysis
On days 0 and 7, cells were suspended in 100 µl PBS containing FITC-conjugated anti-CD34 (1:10; cat. no. 343604; BioLegend) and phycoerythrin (PE)-conjugated anti-CD38 (1:10; cat. no. 560981; BD Biosciences) for 30 min at 4°C, and were then washed with PBS. Cells were analyzed using Guava Incyte, as outlined above.
Microarray-based gene expression analysis
Total RNA was extracted from purified and untreated CD34+ cells from male and female UCB samples using TRIzol (Ambion; Thermo Fisher Scientific, Inc.) according to the manufacturer's protocols. Agilent Whole Genome Oligo Microarrays (one-color; Agilent Technologies, Inc.) were used to analyze the samples. The RNA was hybridized to the microarray, which was washed and scanned. Finally, the data were extracted using Agilent Feature Extraction Software (version 11.0.1.1; Agilent Technologies, Inc.). Affymetrix GeneChip Human Genome U133 Plus 2.0 was used on an Affymetrix 3000 instrument (fluidics station and scanner; Thermo Fisher Scientific, Inc.) running Gene-Chip operating software (version 11.0; Gene Spring Software; Agilent Technologies, Inc.) to generate gene expression data. Through the Gene Ontology (GO; www.geneontology.gov) function and Kyoto Encyclopedia of Genes and Genomes (KEGG; www.genome.jp/kegg/pathway.html) pathway enrichment analysis, significantly enriched pathways associated with hematopoietic cell proliferation were identified in differentially expressed genes (DEGs) with a fold change ≥two.
FACS validation of microarray expression data
CB samples (100 µl) from males and females were added to 2 ml red blood cell lysis buffer (BD Biosciences), followed by incubation for 15 min at room temperature as recommended by the manufacturer. Subsequently, samples were centrifuged at 300 × g for 5 min at room temperature and re-suspended in 100 µl PBS. Cells (1×106) were stained with FITC-conjugated anti-human CD34 10 µl (1:10; cat. no. 343604; BioLegend) and PE-conjugated anti-human CD127 (1:10; cat. no. 557938; BD Biosciences) or PE-conjugated anti-human CD130 (1:10; cat. no. 555757; BD Biosciences) for 15 min at room temperature, according to the manufacturer's protocol. Analyses were performed with Guava Incyte, as described above.
Statistical analysis
The data were analyzed using SPSS software (version 17.0; SPSS Inc.). Quantitative values are expressed as the mean ± standard deviation unless otherwise specified. One-way analysis of variance with Fisher's protected least-significant differences test as a post-hoc analysis was used for multi-group comparisons. P<0.05 was considered to indicate a statistically significant difference.
Results
Higher CD34+ cell populations in CB MNCs from male infants
The median birth weight of the male infants (3,508 g; n=20) was similar to that of the female infants (3,460 g; n=23; P>0.05; Table I). However, the concentration of CB MNCs was lower in the male group than that in the female group (median, 2.04±0.20 vs. 2.52±0.38×106/ml; P<0.05; Table I). The population of CB CD34+ cells in MNCs from the male group was higher than that in the female group (median, 2.72±0.17 vs. 1.26±0.09%; P<0.05; Fig. 2A). The concentration of CB CD34+ cells in the male group was higher than that in the female group (median, 0.04±0.02 vs. 0.03±0.03×106/ml; P<0.05).
Enumeration of CFUs from sorted CD34+ cells
For the 43 samples processed, the purity of CD34+ cells after separation was >95%, as verified by FACS. The number of CFUs and total cells produced in the male group was higher than that in the female group (118±20 vs. 89±8 and 12.8±2.5 vs. 9.2±3.3×106/ml, respectively; P<0.05; Fig. 2B and C). It was observed that the number of CFU-Mix in the male group was higher than that in the female group (20±5 vs. 9±2; P<0.05). There were no significant differences in the number of CFU-GM (colony forming unit-granulocyte/macrophage), BFU-E (burst forming unit-erythroid) or CFU-E (colony forming unit-erythroid) colonies formed between the two groups (P>0.05; Fig. 2D).
Male CB CD34 +cells exhibit better amplification
CB CD34+ cells in the male group exhibited a better amplification efficiency than those in the female group on day 7 (P<0.05; Fig. 3A). The forward vs. side scatter diagrams of the male and female group were gated on the cell population (Fig. 3B). According to FACS analysis, there were no significant differences in the fractions of CD34+CD38− cells between the male and female groups on day 0 (2.0±0.14 vs.1.5±0.07%; P>0.05) and day 7 (21.7±2.0 vs. 18.5±1.1%; P>0.05; Fig. 3C and D).
Microarray-based gene expression analysis
GO enrichment analysis
Agilent Whole Genome Oligo Microarrays containing sequences from 13,594 genes were used. Overall, 64.9% of all genes were differentially expressed in both male and female groups. The up- and downregulated genes of the male and female groups were displayed as a heat map (Fig. 4). In the male group, a total of 1,205 genes (8.8% of total genes) were upregulated by at least 2.0-fold. Upregulated (>6 fold) DEGs are summarized in Table II, and >30% were associated with Y chromosome function. In the female group, 1,313 genes (9.6% of total genes) were upregulated by at least 2.0-fold. Upregulated (>6 fold) DEGs are summarized in Table III. To examine the specific function and biological pathways of the DEGs identified, GO and KEGG pathway analysis was performed using microarray dataset using the GO (www.geneontology.gov) and KEGG pathway database (www.genome.jp/kegg/pathway.html). Among these DEGs, the top five significantly enriched biological processes in the male group were sister chromatid segregation, chromosome segregation, neural precursor cell proliferation, mitotic sister chromatid segregation and positive regulation of cell proliferation (Fig. 5A). The top five significantly enriched biological processes in the female group were platelet activation, response to wounding, wound healing, cell activation and blood coagulation (Fig. 5B).
UniProtKB analysis
Among the DEGs, 2,368 genes in the male group and 3,011 genes in the female group were successfully mapped to the UniProtKB IDs. Differences between the male and female group were categorized into six common biological functions: Cell adhesion, growth, immunity, response to stimulus, proliferation and other. It is noteworthy that a number of genes were involved in the response to stimulus pathway in males and females (37 vs. 45%; Fig. 6), suggesting that hematopoietic stem/progenitor cells are likely to be resistant to unfavorable growth environments, which is consistent with recent studies (12–14).
Signaling pathways
The top three significantly enriched signaling pathways identified in the male group were arginine biosynthesis, hematopoietic cell lineage and cytokine-cytokine receptor interaction, while the top three significantly enriched signaling pathways in the female group were graft-vs.-host disease, malaria and African trypanosomiasis (Fig. 7). The top 10 upregulated signaling pathway in the male and female group are listed in Tables IV and V, respectively. Signaling pathways associated with hematopoietic cell proliferation were screened and analyzed. Subsequent analysis focused on the hematopoietic cell lineage and cytokine-cytokine receptor interaction signaling pathways in the two groups.
In the hematopoietic cell lineage signaling pathway, the male group exhibited high expression of CD5, CD8B, CD20, CD21, CD24, CD126, CD127 and IL-7 (Fig. 8A), which are mainly associated with lymphocyte function. By contrast, the female group exhibited high expression of CD41, CD42, CD61 and thrombopoietin (TPO), which are mainly associated with platelet function (Fig. 8B).
Regarding cytokine-cytokine receptor interaction signaling pathways, the C-X-C motif chemokine ligand 12 (CXCL12)/C-X-C motif chemokine receptor 4 (CXCR4) pathway was upregulated in the male group and the expression levels of IL-6 signal transducer (IL6ST) [glycoprotein (gp)130] and IL-7 receptor (IL-7R) were significantly higher in cells from the male than in the female group (Fig. 9A). However, in the female group, more cytokines were expressed in CD34+ cells, including C-C motif chemokine ligand 2 (CCL2), CCL3, CCL4, CCL5, CXCL3, CXCL6, CXCL7, CXCL10, IL1A, IL1B, IL6, TPO and tumor necrosis factor (TNF) (Fig. 9B).
Higher expression of gp130 (CD130) and IL-7R (CD127) in male CD34+ cells
In order to verify the results of the gene chip analysis, FACS was used to validate the expression of two key molecules in the male group, namely IL-7R (CD127) and gp130 (CD130). FSC/SSC scatter diagrams for the male and female groups were gated on the overall cell population (Fig. 10A). The proportion of CD34+CD127+ cells and CD34+CD130+ cells were higher in the male group compared with the female group. P<0.05; Fig. 10B-D). Statistical analysis of the expression of CD34+CD130+ or CD34+CD127+cells in the male and female groups was detected by flow cytometry (Fig. 10E and F). These results are consistent with those following gene chip analysis.
Discussion
The present study attempted to determine whether neonatal gender affects the hematopoietic potential of CB transplants and, if so, to determine the underlying molecular mechanisms. The experimental results revealed a difference in the concentration of CD34+ cells among CB MNCs between the male and female groups prior to amplification. In the colony formation assay, the number of CFUs and total cells in the male group were higher than those in the female group. Furthermore, the male group exhibited a higher number of CFU-Mix, and this result is consistent with that of a previous study (8). Therefore, it may be hypothesized that gender is a factor influencing HSC amplification.
In the next expansion experiment, it was observed that the cells in the male group exhibited consistently higher amplification efficiencies. However, the percentage of CD34+CD38− cells was not significantly different between the male and female groups.
The gene chip assay indicated that neonatal gender affected the gene expression in CD34+ cells. In terms of the underlying mechanisms, the experimental results indicated that a large number of the upregulated DEGs were involved in chromosomal segregation in the male group. Further study focused on the analysis of signaling pathways associated with hematopoietic cell proliferation. The hematopoietic cell lineages and cytokine-cytokine receptor interaction signaling pathways were upregulated in the male group. Further analysis indicated that the genes CD5, CD8B, CD20, CD21, CD24, CD126, CD127 and IL-7 were highly expressed in the male group. CD5 and CD8B belong to the T-cell antigen phenotype, and CD20, CD21 and CD24 belong to the B-cell antigen phenotype. Regarding cytokine-cytokine receptor interaction signaling pathways, DEGs were upregulated in the CXCL12/CXCR4, IL6ST (gp130), IL-7 and IL-7R pathways in the male group. Based on the above analysis, it was hypothesized that the reasons for the better expansion of CD34+ cells in the male group may be as follows: i) The male group had a higher expression of genes associated with chromosome segregation. Combined with the fact that DEGs involved in the positive regulation of cell proliferation were upregulated, it was speculated that male CB CD34+ cells have more opportunities to enter the mitosis period, which is conducive to cell proliferation. This may explain for the observation that, under the same culture conditions, CB CD34+ cells from males exhibited higher amplification efficiencies than those from females. ii) DEGs associated with lymphocyte proliferation, activation and other functions, including CD5, CD8B, CD20, CD21, CD24, CD126, CD127, IL-7 and IL-7R, were highly expressed in the male group. The major physiological role of IL-7 is to promote T/B lymphocyte development. IL-7 may promote the growth of B-progenitor cells, thymocytes and peripheral mature T cells derived from human or mouse bone marrow (15). According to FACS, CD127 antigens were more highly expressed in male CB CD34+ cells. These results were consistent with those of the gene chip assay. iii) The expression of IL6ST (gp130) was obviously higher in the male group. According to previous studies (16–18), peripheral blood (PB)-derived CD34+ cells ubiquitously express gp130 (19). Gp130 forms the β subunit of IL-6R, which is composed of the IL-6 α-receptor and the β-subunit glycoprotein 130. It is well documented that signals activated by IL-6 are transmitted through the signal-transducing gp130 (20). The physiological role of IL-6 is to stimulate B and T cell proliferation and secretion of antibodies (21). The expression of CD5, CD8B, CD20, CD21, CD24, CD126, CD127, IL-7, IL-7R and IL6ST were significantly higher in male CB CD34+ cells. According to FACS, CD130 membrane surface antigens were more highly expressed in CD34+ cells from male CB. These results were consistent with those of the gene chip. They suggest that there may be more lymphoid progenitor cells in the CD34+ HSCs and hematopoietic progenitor cells (HSP) population, or that CD34+ cells of the male group more easily differentiated into lymphoid progenitor cells. iv) Finally, the CXCL12/CXCR4 pathway was upregulated in the male group. Mice lacking either CXCL12 or CXCR4 exhibit similar embryonic lethal defects, including impaired myeloid and B-cell hematopoiesis (22–24). CXCL12 is a crucial factor involved in the migration, retention, and mobilization of HSCs during homeostasis and after injury, and its corresponding receptor is CXCR4. Collectively, the genetic and functional data indicate that the CXCL12/CXCR4 pathway is crucial for the retention and maintenance of adult HSCs (25). Therefore, it may be speculated that the upregulation of this signaling pathway in the male group is conducive to the maintenance of HSC characteristics and functions. Together, these four points may partially explain why the CD34+ cells from CB of male neonates exhibit an increased amplification capacity.
In the female group, the results revealed an increased expression of platelet phenotype-associated genes, including CD41, CD42, TPO and CD61, suggesting that the CD34+ cells from CB of female neonates may exhibit increased platelet activation and coagulation functions. In addition, female cells expressed more chemokines, including CCL2, CCL3, CCL4, CCL5, CXCL3, CXCL6, CXCL7, CXCL10, 1L1A, 1L1B, IL6, TPO and TNF. This may indicate that the CD34+ cells in the female group have a better migration/chemotaxis ability than those in the male group.
In conclusion, the present study indicated that gender may affect the hematopoietic potential of CB. Whether this difference has any clinical consequences remains to be determined. Careful selection of the best CB units for transplantation may improve the efficiency of this source of HSCs and HSPs in adult transplantation settings and reduce the cost of processing (26). The experimental results of the present study may therefore provide a reference for the clinical selection of CB and insight into potential treatments for certain types of hematological disorder.
Acknowledgements
Not applicable.
Funding
The current study was supported by grants from the Shandong Province Key R&D Fund (grant nos. 2017GSF18155 and 2017GSF218015), the Shandong Province Natural Science Fund (grant no. 2014ZRE27630), the Ji'nan Science and Technology Development Plan (grant no. 201704066) and the National Natural Science Foundation of China (grant no. 81473484).
Availability of data and materials
The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
Authors' contributions
DL and XJ contributed to the study design. PZ, XL, XX and QS performed the experiments. LZ, ZC and XZ performed the statistical analysis and interpreted the data. LZ prepared the manuscript. All authors read and approved the final manuscript.
Ethical approval and consent to participate
The current study was approved by the Ethics Committee of Shandong University Qilu Hospital (Jinan, China). Written informed consent was obtained from all donors.
Patient consent for publication
Not applicable.
Competing interests
The authors declare that they have no competing interests.
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