Microarray analysis of the time‑dependent expression profiles of long non‑coding RNAs in the progression of vein graft stenotic disease
- Authors:
- Published online on: April 15, 2021 https://doi.org/10.3892/etm.2021.10067
- Article Number: 635
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Copyright: © Shen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Abstract
Introduction
Coronary artery bypass grafting (CABG) remains the primary treatment for patients with unprotected left main and multi-vessel coronary artery disease (1). Although total arterial revascularization has been advocated, the autologous saphenous vein is still the most commonly used graft owing to its availability and length (2). However, the per-graft occlusion rate of the vein graft is estimated to be up to 25% during the first 12 months following CABG, and almost half of the venous conduits fail at 10 years (3).
Clinically, vein graft stenotic disease leads to considerable post-operative adverse events, including recurrent angina, myocardial infraction and repeat revascularization (4). To date, the pathophysiological mechanisms underlying venous wall remodeling remain to be fully understood. Thrombosis, neointimal hyperplasia and superimposed atherosclerosis are generally regarded as primary causes of vein graft failure that have attracted the most research interest for therapeutic applications (5). To the best of our knowledge, however, apart from statins and antiplatelet agents, no other interventions have proven to be clinically effective (6). Considering the aging population and the persistent increase in the incidence of patients with CABG living with symptomatic vein graft stenotic disease in Europe and USA (7-9), further identification of novel biomarkers that can be effectively targeted is urgently required (10).
There has been an increasing interest in the role of non-coding RNAs in the pathogenesis of vascular disease (11). Based on transcript size, non-coding RNAs are classified into small, medium and long non-coding RNAs (lncRNAs) (12). The biogenesis and functional properties of small RNAs, particularly microRNAs (miRNAs or miRs), in the onset and progression of cardiovascular disease have been well-described (13). For example, the administration of miR-221 has been shown to effectively inhibit endothelial cell proliferation at predilection sites (14). However, the expression profiles and potential functions of lncRNAs in the progression of venous wall remodeling following bypass grafting remain unknown. The present study aimed to investigate the dynamic expression profiles of lncRNAs following bypass grafting in a rat model, and to elucidate the potential association between lncRNAs and vein graft stenotic disease.
Materials and methods
Rat model establishment
A total of 12 male Sprague Dawley rats (age, 6-8 weeks) were purchased from the Laboratory Animal Center of Sichuan University (Chengdu, Sichuan, China). They were randomly divided into four groups to ensure equal weight distribution (weight, 248±27 g). One group receiving sham surgery was set as the control (day 0; n=3), whilst the other three groups were set as the experimental groups according to different time points after vein bypass grafting (7, 14 and 28 days after surgery, respectively; n=3 per group). For the experimental groups, the rats were anesthetized with intraperitoneal injection of 10% chloral hydrate (350 mg/kg). Heparin (100 U/kg) and cefazolin (50 mg/kg) were administered intraperitoneally for anticoagulation and infection prevention, respectively. No signs of peritonitis were observed. A length of vein graft (1 cm) was harvested from the external jugular vein and preserved in heparinized saline (25 U/ml). The vein was subsequently grafted to the carotid artery (Fig. 1). For the control group, all the procedures performed were identical with those in the experimental groups except vein bypass grafting was not peroformed. These rats were housed in a temperature-controlled room at 20±2˚C and relative humidity of 50%, with a 12-h light/dark cycle. Free access to food and water was provided. These experiments were performed according to the National Institutes of Health Guideline for the Care and Use of Laboratory Animals (15). The present study was approved by the Animal Ethical and Welfare of Sichuan University, Sichuan, China (approval no. 2018103A).
Total RNA extraction and array hybridization
Following the intraperitoneal administration of 10% chloral hydrate (350 mg/kg) for anesthetization, the rats were sacrificed by cervical dislocation. Vein samples (~1 cm) were harvested from the different groups. Total RNA was isolated using TRIzol® reagent according to the manufacturer's instructions (Invitrogen; Thermo Fisher Scientific, Inc.). The purity of the RNA samples was analyzed using the Nanodrop spectrophotometer (Implen GmbH), which measured absorbance at 230, 260 and 280 nm. The 260/280 and 260/230 absorbance ratios were calculated to evaluate RNA purity (A260/A280 ratios are 1.8-2.2 and A260/A230 ratios are over 1.7). 1.0% non-denaturing agarose gel electrophoresis was used to determine the RNA integrity. The microarray analysis was performed by Kangchen BioTech Co., Ltd. using Agilent Technologies Rat LncRNA Microarray scanner (cat no. G2505C; Agilent Technologies, Inc.). Sample labeling and microarray hybridization were performed according to the manufacturer's protocols. In brief, ribosomal RNA was removed from total RNA using a mRNA-ONLY™ Eukaryotic mRNA Isolation kit (cat. no. MOE51010; Zhongbei Linge Biotechnology, Ltd.; http://www.bjzblg.com/product/18395.html). The remaining RNA was amplified and reverse transcribed into fluorescent complementary (c)RNA using the PrimeScript™ RT Reagent Kit (cat. no. RR037B; Takara bio, Inc.) at 37˚C for 15 min, followed by 85˚C for 5 sec. The RNeasy Mini kit and NanoDrop ND-1000 instrument were used to purify and measure the concentration of the labeled cRNAs. A total of 5 µl 10X Blocking Agent and 1 µl 25X Fragmentation Buffer were mixed with 1 µg labeled cRNA and the mixture was then heated at 60˚C for 30 min. Subsequently, 25 µl 2X GE Hybridization buffer was added to dilute the labeled cRNA. The hybridization solution was incubated for 17 h at 65˚C in an Agilent Technologies Hybridization oven. After washing, the hybridized arrays were scanned with using an Agilent microarray scanner (cat no. G2505C; Agilent Technologies, Inc.).
Bioinformatics analysis of microarray data
Raw data were extracted from the acquired array images using Agilent Feature Extraction software 11.0.1.1 (Agilent Technologies, Inc.). GeneSpring GX v12.1 software (Agilent Technologies, Inc.) was used to normalize quantiles of raw data. Genes with an adjusted P-value <0.01 and an absolute value of log2 (fold change) >2.0 were considered to be significantly differentially expressed. Hierarchical clustering analysis was performed to observe the different clustering patterns of lncRNAs and mRNAs between the control and experimental groups. Scatter and volcano plots analysis were constructed to express the differentially expressed genes. In order to examine the distribution of differentially expressed lncRNAs, histograms and a pie chart were generated to reflect the chromosomal distribution, lengths and regulation mechanisms of the differentially expressed lncRNAs.
Prediction and functional enrichment analysis of lncRNA target genes
The protein-coding genes targeted by lncRNAs were predicted via ce- and trans-regulation analyses, respectively. In the ce-regulation analysis, lncRNAs compete with shared miRNAs to regulate target protein-coding genes, which are genomically neighboring within 10 kb. In the trans-regulation analysis, lncRNAs regulate protein-coding genes sequences matched with low complement energy (inclusion criteria, G<-20). Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) enrichment analyses were performed to evaluate the functional pathways and biological relevance of the lncRNA co-expressed mRNAs using Database for Annotation, Visualization and Integrated Discovery (david.abcc.ncifcrf.gov/). All functional analyses were performed using Enrichr (16).
Co-expression network analysis
The co-expression of lncRNAs and protein-coding genes was assessed using Gephi 0.9.2(17) and Pearson's correlation test (P<0.05). Upregulated lncRNAs were presented as orange nodes and downregulated lncRNAs were presented as blue nodes. Protein-coding genes targeted by lncRNAs were presented as green nodes.
Verification of microarray data by reverse transcription-quantitative (RT-q)PCR
RT-qPCR was performed to validate the microarray data. Samples were divided into control group and experimental groups. Samples of control group were collected from rats receiving sham surgery (n=3), samples of experimental groups were collected from rats that received vein bypass grafting (7, 14 and 28 day after surgery, respectively; n=3 per group). Total RNA was extracted with TRIzol® reagent from the remaining portion of tissues not used for the lncRNA microarray. Subsequently, first-strand complementary DNAs were generated using SuperScript™ III Reverse Transcriptase (Invitrogen; Thermo Fisher Scientific, Inc.) following the manufacturer's protocols. The RT-qPCR process was performed using a ViiA-7 RT-PCR System (Applied Biosystems; Thermo Fisher Scientific, Inc.) and Arraystar SYBR-Green Real-time qPCR Master Mix (cat no. AS-MR-005-25; Arraystar, Inc.). The reactions were performed under the following conditions: Initial denaturation at 95˚C for 30 sec, followed by 40 cycles of 95˚C for 5 sec and 60˚C for 30 sec. The relative expression levels of lncRNAs were quantified using the 2-ΔΔCq method (14). β-actin served as an internal control. The primers for RT-qPCR were designed according to the lncRNA sequences in NONCODE (version v6.0; http://www.noncode.org) and primer sequences were synthesized and purified by Kangchen BioTech Co., Ltd. The primer sequences are presented in Table I. All experiments were performed in triplicate for error reduction.
Cells and cell culture
After the rats which were used for lncRNA microarray were sacrificed by cervical dislocation, primary vascular smooth muscle cells (VSMCs) were obtained from normal external jugular vein. All procedures were performed according to a previously published protocol (18). In brief, external jugular vein was isolated from adherent tissue, and adventitia and endothelium were denuded. Pieces of vein (1 mm2) were cultured in serum-free DMEM/F12 (Gibco; Thermo Fisher Scientific, Inc.) supplemented with 20% FBS (Gibco; Thermo Fisher Scientific, Inc.) and 1% penicillin-streptomycin at 37˚C in a 5% CO2 humidified incubator. Cells migrated from the sections of aorta after 7-9 days. Vein pieces were then removed and VSMCs were left to proliferate. Experiments used VSMCs from passages 3-6 at 80-90% confluence.
Infection with lentiviral vector and cell proliferation assay
VSMCs suspensions were seeded into six-well plates at a density of 4x105 cells/well. DMEM/F12 (Gibco; Thermo Fisher Scientific, Inc.) with 10% v/v FBS (Gibco; Thermo Fisher Scientific, Inc.) was removed the following day and replaced with virus concentrated liquid (Shanghai OBiO Technology Co., Ltd.). Stable overexpression of Src was achieved by construction of the GL107 pSLenti-EF1-EGFP-P2A-Puro-CMV-MCS-3xFLAG-WPRE lentivirus (Shanghai OBiO Technology Co., Ltd.), containing the AF062402, one of the significantly upregulated lncRNAs, gene vector with green fluorescence protein (GFP; Shanghai OBiO Technology Co., Ltd.) to detect transfection efficacy. Polybrene reagent (Shanghai OBiO Technology Co., Ltd.) was diluted to a final concentration of 5 µg/ml. Multiplicity of infection (MOI) values (1, 2, 5, 10, 30 and 50) were then tested on VSMCs. A total of 4x106 lentivral particles were used for transfection and the optimal MOI value was 10 in the present study. At this value, the efficiency of infection reached 80% 3 days post-infection. After swirling the plate gently to mix the cells, the plate was placed in an incubator with 5% CO2 at 37˚C for 24 h. After 24 h, the virus concentrated medium was removed and replaced by DMEM/F12 medium (Gibco; Thermo Fisher Scientific, Inc.) with 10% FBS (Gibco; Thermo Fisher Scientific, Inc.). At 3 days post-transfection, GFP expression was observed as the lentivirus was integrated into the VSMC genome, in five randomly-selected fields using a fluorescence microscope (magnification, x200). Cells with a transfection efficiency >80% on day 3 were selected for subsequent analysis. VSMCs transfected with AF062402 lentiviral expression vector were set as the AF062402 intervention group. Non-transfected cells were set as the blank control and lentivirus without the AF062402 gene was transfected as the negative control (NC). At 72 h following transfection, RT-qPCR was performed to verify the expression levels of AF062402 in the different groups. Moreover, Cell Counting Kit-8 (CCK-8) assay was performed using a kit from Abcam. VSMCs were digested into single cell suspensions and seeded into 96-well plates (5x103 cells/ml). After 48 h, CCK-8 solution (10 µl/well) was added and the plates were incubated for 2.5 h at 37˚C before the optical density (OD) value was detected at 460 nm using a plate reader. All experiments were performed in triplicate.
Statistical analysis
Feature Extraction software (Agilent Technologies, version 11.0.1.1) was used to analyze the acquired array images. Differentially expressed lncRNAs and mRNAs were identified by adjusted P-value <0.01 and absolute value of the log2 (fold change) >2.0. The results of CCK-8 assay and RT-qPCR are expressed as the mean ± SD (n=3). Statistical comparisons between groups were performed using one-way analysis of variance with Tukey's post hoc multiple comparison test. P<0.05 was considered to indicate a statistically significant difference.
Results
Overview of microarray data and expression patterns of lncRNAs
A total of 8,041 lncRNAs and 13,001 mRNAs were detected. Box plots and hierarchical clustering analysis (Fig. S1) provided an overview of all normalized genes in all samples. As shown in the hierarchical clustering analyses, scatter and volcano plots, distinct expression profiles of lncRNAs and mRNAs at different post-operative time points were observed (Figs. 2A and S2). The lncRNAs were then classified based on chromosomal distribution and length (Fig. 2B and C). Subsequently, 13 lncRNA profiles with statistical significance were selected for further investigation (Fig. 2D). A total of 2,572 lncRNAs and 1,652 associated mRNAs with significant changes in expression levels (P<0.01; fold change >2) were detected. lncRNAs were categorized into five subgroups (Fig. 2E) according to their association with adjacent genes as follows: i) Intergenic lncRNAs, 27.17%; ii) exonic-antisense and exonic-sense lncRNAs, 5.65 and 23.66%, respectively; iii) intronic-antisense and intronic-sense lncRNAs, 6.64 and 9.28%, respectively; iv) lncRNAs transcribed on the opposite strand of a coding gene, 1.90% and v) the remaining lncRNAs transcribed in other patterns, 27.29%. The numbers of lncRNAs with significantly upregulated or downregulated expression at different post-operative time points are presented in Fig. 2F. Moreover, the top 10 lncRNAs with significant changes in expression levels at different post-operative time points are presented in Table II.
lncRNA target prediction and functional annotation
In order to identify the potential functional pathways of lncRNAs with significant changes in expression levels, ce- and trans-regulation analysis results were combined and overlapping mRNAs were selected for further ‘GO’ and ‘KEGG’ analyses. ‘GO’ analysis revealed a significant enrichment of 4,993 ‘GO’ terms (P<0.01), of which ‘cellular process’, ‘protein binding’ and ‘cell/cell part’ were the top-listed terms involved in biological process, molecular function and cell component, respectively (Fig. 3A). ‘KEGG’ analysis revealed that 298 biological pathways were enriched (P<0.01), of which several were associated with vascular remodeling, including ‘cell adhesion molecules’, ‘platelet activation’ and ‘vascular smooth muscle contraction’ (Fig. 3B). Among these, nine biological processes associated with vascular remodeling process were selected for further analysis, including ‘cell adhesion’, ‘cell migration’, ‘cell proliferation’, ‘MAP kinase activation’, ‘platelet activation’, ‘chemotaxis’, ‘WNT protein secretion’, ‘stimulation responses’ and ‘growth factor activity’. The changes in the numbers of lncRNAs in these selected biological processes are presented in Fig. 4A.
Co-expression network analysis of lncRNAs and associated protein-coding genes
Based on the results of functional analysis, a total of 360 lncRNAs and 135 associated protein-coding genes were predicted to be involved in the vascular remodeling process (Table SI). Among these, 194 lncRNAs and seven previously reported associated protein-coding genes (19-32) involved in mediating vascular disease were selected to construct a co-expression network. Each protein-coding gene was targeted by multiple lncRNAs (Fig. 4B).
RT-qPCR validation and stimulation of VSMC proliferation via overexpression of AF062402
A total of three lncRNAs and associated protein-coding genes were randomly selected to verify the microarray data by RT-qPCR. AF062402 and Src were upregulated, whereas BC091437 and Edg1 were downregulated (Fig. 5A). Moreover, the upregulation of BC166461 was accompanied by downregulation of melanoma cell adhesion molecule (Mcam). The relative expression levels of the selected lncRNAs and associated protein-coding genes were consistent with the microarray data. Moreover, RT-qPCR confirmed that the relative expression of AF062402 in the Lentivirus-AF062402 group was significantly higher than that in the blank and Lentivirus-NC groups. The overexpression of AF062402 stimulated the proliferation of primary VSMCs compared with the other two groups (Fig. 5B).
Discussion
Vein graft restenosis is a primary long-term complication which leads to a poor prognosis of patients with CABG (10). However, apart from statins and antiplatelet agents, few therapeutic strategies have been proven to have significant clinical efficacy (6). Multiple studies have demonstrated that lncRNAs serve key roles in the pathophysiology of various diseases by participating in numerous regulatory processes, such as DNA methylation, histone modification, basal transcription, post-transcriptional processes and protein binding (33,34), indicating that lncRNAs may be potential intervention targets for vein graft restenosis.
The present study systemically screened a genome-wide microarray profile of lncRNA and mRNA expression at different time points following the establishment of rat autogenous vein bypass grafts. A total of 8,041 lncRNAs and 13,001 mRNAs were detected. In total, 2,572 dysregulated lncRNAs were screened following vein bypass grafting and lncRNA-associated protein coding genes were predicted by ce- and trans-regulation analysis. Through enrichment analysis, nine biological processes associated with the vascular remodeling process were selected. Moreover, co-expression network analysis revealed an association between lncRNAs and their target genes, which were reported to play roles in mediating vascular disorders (19-32). Furthermore, it was found that AF062402, one of the significantly upregulated lncRNAs, may serve a role in inducing vein graft restenosis.
Various pathophysiological changes secondary to vascular bypass grafting, such as inflammation, platelet activation, stimulation responses, leukocyte chemotaxis and mechanical intimal injury, are regarded as primary causes of vein graft restenosis (35-37). The inflammatory and thrombotic cascade responsible for early graft thrombosis and failure can be caused by direct endothelial injury or endothelial activation (38). Intimal hyperplasia and atherosclerosis are also considered to be primary causes of lumen remodeling of the graft, which are associated with the processes of leukocyte adhesion and vascular endothelial cells and VSMC proliferation (35). Several studies have implicated the production of reactive oxygen species in disease progression in grafts (39,40). There is increasing evidence to demonstrate a pivotal role of MAPK activation cascades in vascular restenosis, which contribute to proliferation of VSMCs and neointima formation via downstream proteins (41). It is also known that leukocyte chemotaxis serves a key role in a complex mechanism, involving an association between inflammation factors, platelets and vascular disease (36). Moreover, exposure to high pressure of arterial circulation results in early, diffuse intimal thickening as a compensatory response termed arterialization (37). With enhanced understanding of the functions of lncRNAs in regulating transcription, lncRNA-mRNA pairs were predicted to be involved in vein graft failure following CABG in the present study. The association between lncRNA and mRNA was the focus of the present study.
As shown in the present study, four protein-coding genes were targeted by both upregulated and downregulated lncRNAs. Edg1, a specific cell surface G-protein coupled receptor, stimulates the activation of multiple cellular events, such as cell proliferation and migration, and the expression of adhesion molecules via S1P signaling (42). c-Src, a member of the Src family, is involved in multiple signaling mechanisms; it not only serves a critical role in mediating the migration and proliferation of VSMCs via the c-Src/protein kinase C (PKC)/MAPK signaling pathway, but is also essential in inducing the expression of Edg1, which is hypothesized to contribute to the progression of intimal thickening following vascular injury in VSMCs (20,21). Overexpression of AF062402 in the present study stimulated the proliferation of VSMCs, verifying the potential therapeutic effect of AF062402-Src in mediating VSMC proliferation. For patients with CABG with diabetes mellitus or hyperlipemia, insulin receptor (Insr) may be a therapeutic target. According to a previous study, overexpression of Insr-A, one of the isoforms of Insr, confers a proliferative advantage on VSMCs in the early stages of the atherosclerotic process (43). Furthermore, decreased Insr-A/Insr-B ratio may also contribute to apoptosis of VSMCs and increased risk of atherosclerotic plaque rupture (22). Mcam, an endothelial transmembrane protein of the immunoglobulin superfamily, has been reported to be involved in several important signaling pathways in vascular disease, including PI3K/AKT, p38 and PKC (23). The interaction between Mcam and VEGFR-2 on the endothelial cell surface stimulates cell migration via the AKT and p38 pathways (24). Mcam can also interact with galectin-1 to protect against galectin-1-induced endothelial cell apoptosis (25). Moreover, a previous study revealed a causative role of Mcam in activating lipid-loaded macrophages in plaques, indicating the potential ability of Mcam to be a novel therapeutic target in vascular remodeling following CABG (26).
TGFβ-1 and Fes are two protein-coding genes shown to be targeted by upregulated lncRNAs in the present study. TGFβ-1 is the central regulator of the canonical SMAD2/3 pathway in mediating the deposition of extracellular matrix, which leads to fibrosis in vascular injury (27). A previous study also indicated that TGFβ-1 is involved in regulating the expression of CD40, which serves as the central regulator of various immune responses (28). TGFβ-1 may be responsible for heterogeneous expression of CD40 at the site of vascular bifurcations or curvatures, particularly in the vein grafts of patients with CABG. Fes, one of the known members of a subfamily of the non-receptor tyrosine kinase family, was targeted by 32 upregulated lncRNAs in the present study. Activated Fes kinase has been reported to shift the equilibrium of STAT3, STAT5 and extracellular-signal regulated kinase/MAPK activation to facilitate the adhesion and chemotaxis of inflammatory cells and regulate the proliferation of vascular endothelial cells. Upon phenotypic transition, Fes assists in smooth muscle cell migration to the neo-intima (29).
Yes1 associated transcriptional regulator (Yap) was a protein-coding gene targeted by downregulated lncRNAs in the present study. As a downstream effector, Yap is one of the vital components of the Hippo/Yap pathway, which has been reported to serve roles in mediating vascular restenosis in rodent models (30). Overexpression of Yap combined with intima-media thickness has also been observed in a rat carotid artery balloon injury model (31). In addition, elevated activity of Yap has been reported to mediate the switch of the VSMC phenotype to the synthetic state, promoting neointima formation (32). Moreover, thromboxane A2-specific agonists have been found to induce VSMC migration and proliferation by activating Yap (19). These results demonstrate a potential therapeutic approach for vascular remodeling by activating the Hippo pathway or inhibiting the expression of Yap.
Several limitations of the present study need to be acknowledged. First, since microarray can only detect known gene sequences, certain lncRNAs involved in the vascular remodeling process remain to be investigated. Second, lack of knowledge of the properties and functional mechanisms of lncRNAs involving gene expression meant the role of a specific lncRNA in an overt biological process according to its expression level could not be accurately predicted. Third, although several lncRNAs with potential association in vein graft failure were identified via co-expression network analysis, the detailed mechanisms of these lncRNAs remain unclear; further validation experiments, including western blot analysis, immunohistochemistry and immunofluorescence staining, are required to determine the casual association between these lncRNAs and protein-coding genes overlapped with mRNAs.
In conclusion, the present study provides preliminary evidence of lncRNA expression profiles in vein graft restenosis following vascular grafting. Even though further validation experiments are still required, lncRNAs and target protein-coding genes predicted in the present study may serve as potential therapeutic targets for vein graft restenosis following CABG.
Supplementary Material
Microarray data quality control. (A) Box plots showing the symmetry of data and the degree of dispersion. The ordinate represents the fold change (log2) in signal values of probes. (B) Heatmaps revealing all differentially expressed lncRNAs and mRNAs. Red in heatmap indicates increased expression; green in heatmap indicates decreased expression. Purple, green, blue and red on the top of heatmap indicate day 0, 7, 14 and 28, respectively. lncRNAs, long non-coding RNAs.
Heatmaps showing mRNAs that were differentially expressed between groups. Scatter and volcano plots were generated to show the variations in mRNA expression between different time points (0 vs. 7, 14 and 28 day). Red and green in heatmap indicate increased and decreased expression, respectively; blue on the top of heatmap indicates the control group (day 0); red on the top of heatmap indicates the experimental group (day 7, 14 and 28, respectively); orange and blue in scatter plots indicate significantly and non-significantly expressed mRNAs; red and grey in volcano plots indicate significantly and non-significantly expressed mRNAs.
Acknowledgements
Not applicable.
Funding
Funding: The present study was funded by the National Natural Science Foundation of China (grant nos. 81670327 and 81300155), the Commission of Science Technology of Sichuan Province (grant nos. 2019YJ0046 and 2018SZ0384) and the Clinical Research Incubation Project of West China Hospital of Sichuan University (grant no. 2019HXFH027).
Availability of data and materials
The datasets generated and/or analyzed during the current study are available in the NCBI Gene Expression Omnibus repository, accession no. GSE152538.
Authors' contributions
HZ and JS confirm the authenticity of all the raw data. HZ established the animal model. YZ performed the cell culture experiments. JS and CL analyzed the data and wrote the manuscript. JG and JH contributed to put forward the conception and helped to revise the manuscript. All authors read and approved the final manuscript.
Ethics approval and consent to participate
The present study was approved by the Animal Ethical and Welfare of Sichuan University, Sichuan, China (approval no. 2018103A).
Patient consent for publication
Not applicable.
Competing interests
The authors declare that they have no competing interests.
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