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Introduction

Mono-adenosine diphosphate (ADP)-ribosyltransferases (ARTs) have been noted to catalyze ADP-ribosylation, which transfers an ADP-ribose from nicotinamide adenine dinucleotide (NAD+) to various amino acid residues, thus altering protein function and playing an important role in physiological and pathological processes such as signal transduction, cellular differentiation and proliferation, protein secretion and transport, cellular necrosis and apoptosis (1). Seven mono-ADP-ribosyltransferases (ART1-ART7) have previously been found in mammalians, and five members (ART1, ART2, ART5, ART6 and ART7) are specific to arginine (2,3). ART1 was first discovered in bacteria, viruses and eukaryotic cells (4), and it was subsequently found in human skeletal muscles, cardiac muscle cells, white blood cells and airway epithelial cells (5–7). The gene which codes for ART1 is located on human chromosome 11P15, and its target proteins include integrin α7, αL (CD11a), β2 (CD18), human neutrophil peptide-1 (HNP-1), basic fibroblast growth factor (bFGF) and platelet-derived growth factor (PDGF)-BB (8–12).

In non-neoplastic diseases, ARTs are related to the occurrence of acute inflammation of the intestinal mucosa (13). When mono-ADP-ribosylation is inhibited, the release of inflammatory mediators by monocytes and macrophages is inhibited, suggesting that ARTs promote inflammatory development (14). Furthermore, ART1 specifically alters HNP-1 activity, which reduces antibacterial and antitoxic ability of HNP-1 (15). When T lymphocytes are transfected with ART1 in the presence of NAD+, the function of the T cell receptor is altered by the lymphocyte function-associated factor, thus affecting lymphocyte function and suggesting that ART1 acts in an extensive capacity (9,16). Furthermore, gastric cancer researchers have found that Helicobacter pylori modulates ART activity, leading to modifications in membrane conformation and possibly promoting the development of gastric cancer (17). In the lung cancer cell line A549, ART1 is highly expressed; ART1 and ART4 are upregulated upon human Toll-like receptor (hTLR) stimulation, thus suggesting that ARTs are important in the development of lung cancer (18).

Previously we demonstrated that ART1 was highly expressed in human colorectal cancer (CRC), and that its expression was positively correlated with the expression of angiogenic factors such as integrin αVβ3, vascular endothelial growth factor (VEGF) and bFGF (19). It has previously been suggested that ART1 has the ability to promote angiogenesis in CRC. Several previous studies have reported that the phosphoinositide 3-kinase (PI3K)/Akt pathway not only promotes tumor cell proliferation and inhibits apoptosis, but is also closely related to tumor angiogenesis. This mechanism may be involved in the activation of the PI3K/Akt pathway, which subsequently upregulates the expression of hypoxia-inducible factor 1-α (HIF-1α) and promotes transcription and secretion of VEGF, and eventually leads to promote tumor angiogenesis through VEGF binding to vascular endothelial cell surface receptors (20,21). It is unclear whether ART1 is involved in VEGF, bFGF and HIF-1α expression, which is regulated by the PI3K/Akt pathway, in CRC cells thus far.

In the present study, LoVo cells transfected with ART1-cDNA or with ART1-shRNA were co-cultured with human umbilical vein endothelial cells (HUVECs), and subsequently HUVEC proliferation, migration and vascular-forming abilities were all examined. Additionally, the effect of ART1 on tumor microvessel density (MVD) was examined in vivo. The expression levels of total (t-)Akt, phosphorylated (p-)Akt, HIF-1α, VEGF and bFGF were further detected in order to characterize the molecular mechanisms of ART1 during tumor angiogenesis.

Materials and methods

Cell and lentiviral transfection

The LoVo cell line, a human colon cancer cell line, and HUVECs were kindly provided by Professor Wei-Xue Tang, Chongqing Medical University (Chongqing, China). The mouse colon cancer cell line CT26 was kindly provided by Professor Yu-Quan Wei, Sichuan University (Chengdu, China). ART1 was cloned into a pGCSIL-GFP lentiviral vector in order in order to generate both pGCSIL-GFP-vshART1 and pGCSIL-GFP-ART1-cDNA. The ART1-shRNA interference sequence was GCCAACAAAGTATACGCGGAT. The primers of ART1-cDNA used were as follows: primer 1, GAGGAT CCCCGGGTACCGGTCGCCACCATGAAGATTCCTGCTA TGATG; primer 2, TCACCATGGTGGCGACCGGACA TCGGGTAAGTTGCTG. The pGCL-GFP-vshART1 lentiviral vector, ART1-cDNA lentiviral vector and empty lentiviral vector were purchased from GeneChem, Inc. (Shanghai, China). The human CRC cell line LoVo was cultured and transfected according to the manufacturer's instructions (GeneChem, Inc.). LoVo cells were seeded in 24-well plates at a density of 3×104 cells/well, transfected with 10 µl lentiviral vector and observed under a fluorescence microscope 96 h post-transfection until the infection rate reached 80%. Transfection efficiency was detected under a fluorescence microscope (Leica DMI4000B; Leica Microsystems GmbH, Wetzlar, Germany) after 96 h. The efficiency of the ART1-cDNA and ART1-shRNA lentivirus transfected into LoVo cells was detected by reverse transcription (RT)-PCR and western blot analysis. The murine CRC cell line CT26 was cultured and transfected as previously described by Tang et al (22). The group which were transfected with ART1-shRNA lentiviral and the group transfected with ART1-cDNA lentiviral vector were set as experimental groups. The empty lentiviral vector transfected group (LV-control group) and untransfected group were set as control groups.

RT-PCR

Total RNA was separately extracted from untransfected LoVo cells, LV-control LoVo cells, ART1-cDNA- and ART1-shRNA-transfected LoVo cells with TRIzol reagent (Takara, Dalian, China) and reverse transcribed into DNA. ART1 gene expression was detected using oligonucleotide primers which were designed and produced by Sangon Biotech Co. (Shanghai, China): ART1, 5′-ACCTTCTTCGGTATCTGGACCT-3′ (F1) and 5′-TAAGTTGCTGGAGACCTG GATT-3′ (R1); and β-actin acted as the internal control gene, 5′-ATATCGCTGCGCTGGTC GTC-3′ (F1) and 5′-AGGATGG CGTGAGGGAGAGC-3′ (R1). Using a one-step RT-PCR kit (Takara), in total, 30 PCR cycles were used for the amplification of RT products (94°C for 30 sec, 60°C for 30 sec and 72°C for 25 sec). Finally, the amplifiation products were electrophoresed on 2% agarose gels (Genview, Tallahasse, FL, USA). The above experiment was performed in triplicate

Analysis of HUVEC proliferation

In the present study, HUVEC proliferation was detected as previously described (23). The single-cell suspensions of HUVECs were cultured in a 96-well culture plate at a final concentration of 2×103 cells/well in Dulbecco's modified Eagle's medium with 20% fetal bovine serum (both purchased from Gibco-BRL, Gaithersburg, MD, USA) for 24 h. HUVECs were subsequently co-cultured with supernatant fluid of LoVo cells in ratios of 0, 20, 40, 60, 80 and 100% 48 h later. This was followed by the addition of 10 µl of Cell Counting kit (CCK-8; KeyGen Biotechnology, Nanjing, China) reagent to each well, incubation for 4 h and detection at an OD of 450 nm by universal microplate reader (Bio-Tek Instruments Inc., Winooski, VT, USA). The assays were repeated in triplicate.

HUVECs migration analysis

HUVEC migration was detected using 8.0-mm pore size Transwell inserts (Costar, Milpitas, CA, USA) as previously described (24). The Transwell semipermeable membrane was coated with diluted Matrigel (Sigma, Ronkonkoma, NY, USA) incubated at 37°C for 30 min to solidify. LoVo single-cell suspension was seeded into the lower chamber at a concentration of 1×105 cells/well and incubated overnight in RPMI-1640 with 10% FBS (both from Gibco-BRL, Gaithersburg, MD, USA). HUVEC single-cell suspension was seeded in the upper chamber at a concentration of 1×105 cells/well and incubated under conventional culture conditions. At 6 and 12 h later, the cells on the upper surface of the membrane were carefully scraped off and the migrant cells which adhered to the lower surface were fixed with 4% methanol for 15 min at room temperature, and stained with 0.1% crystal violet for 3 min. The number of cells on the lower surface of the membrane was counted in five different fields using an inverted microscope at a magnification of ×200. The experiment was repeated in triplicate.

Analysis of HUVEC lumen-forming ability

A 3D collagen cell culture system (Millipore, Inc., Billerica, MA, USA) was utilized; we proportionally combined collagen solution, 5X medium and neutralization solution, with 150 µl of the mixture added to each well of a 24-well plate. The plate was then incubated at 37°C for 60 min in order to solidify. The HUVEC single cell suspension was then seeded into each well at a concentration of 5×105 cells/well and mixed with different ratios (0, 10, 20 and 50%) of supernatant from transfected LoVo cells, cultured for 72 h and observed under the microscope, with the total length randomly selected for 5 lumen, and the total length was measured (25). The experiment was repeated in triplicate.

Transplanted tumor mouse model

Sixteen BALB/c female mice (Chongqing Medical University Laboratory Animal Center, Chongqing, China), 6–8 weeks old and weighing 18–22 g, were randomly divided into four groups: the group transfected with ART1-shRNA lentiviral vector, the group transfected with ART1-cDNA lentiviral vector, a group transfected with an empty lentiviral and the unransfected group. Mice were inoculated as previously described (26). Following anesthesia an intraperitoneal injection of 2% chloral hydrate (Beijing DingGuo Biological Technology Co., Ltd., Beijing, China), the mice were fixed in a supine position, and an incision was made into the abdominal cavity from the left upper quadrant axillary midline to expose the spleen. The mice were administered the CT26 cells (5×106) in single suspension/mouse via a subcapsular injection into the spleen. Fourteen days later, the mice were sacrificed by cervical dislocation and the metastatic spleen tissues were removed and fixed with 4% paraformaldehyde, and paraffin-embedded tissues were cut into slices for H&E staining and immunohistochemistry.

All animal experiments were performed after obtaining approval from the Ethics Committee of Chongqing Medical University. Appropriate care was taken to minimize animal suffering. The disposition of the animals at the end of the study, euthanisa criteria and all experimental methods involving animals were in accordance with the code of practice for the care and use of animals for scientific purposes.

H&E staining and CD34 immunohistochemical assay

The tissue sections were dewaxed with xylene, and the slides were then soaked in graded concentrations of alcohol (100, 95, 85 and 70%) for 5 min, stained with hematoxylin for 3 min and eosin for 1 min (both from Beijing DingGuo Biological Technology Co., Ltd.) washing with water. Vascular endothelial cells were marked with CD34 (Wuhan Boster Biological Technology, Ltd., Wuhan, China) using an EnVision™ detection kit (DakoCytomation, Genetech, Beijing, China) following the manufacturer's protocols, as previously described (27). According to the method put forward by Weidner et al (27), areas with the most intense coloring were selected as 'hot spots', and the microvessels were counted under the microscope and imaged, with 3 'hot spots' counted per slice and averaged to obtain the slide MVD.

Western blot analysis

LoVo cells from each transfection group were collected; total protein was extracted according to protein extraction protocols (Beyotime P0013; Beyotime Biotechnology Co., Ltd., Shanghai, China). Protein concentrations were determined using a BCA assay kit (Beyotime Biotechnology Co., Ltd.) and equal amounts of total protein were separated via SDS-PAGE and transferred onto a PVDF membrane. The membrane was blocked at room temperature for 1 h, and incubated with the primary antibody overnight at 4°C. The primary antibodies, ART1 (AP2311a; Abgent Biotechnology, Inc. San Diego, CA, USA), HIF-1α (20960-1-AP; Proteintech Group, Inc., Chicago, IL, USA), Akt, p-Akt Thr308 (BS2987 and BS4009, respectively; Bioworld Technology, Co., Ltd., St. Louis Park, MN, USA), VEGF, bFGF and β-actin (PB0084, BA0259 and BA2305, respectively; Wuhan Boster Biological Technology, Ltd.), were diluted to 1:500, 1:1,000, 1:800, 1:600, 1:1,000, 1:1,000 and 1:1,000, respectively. The membrane was washed with TBST and incubated with the secondary antibody (peroxidase-conjugated goat anti-rabbit IgG, peroxidase-conjugated rabbit anti-goat IgG or peroxidase-conjugated goat anti-mouse IgG; ZDR-5306, ZDR-5308 and ZDR-5307, respectively; Beijing ZSGB Biotechnology Co., Ltd.) at a dilution of 1:1000 for 2 h. The image was captured and imaged using a ChemiDoc XRS system (Bio-Rad, Hercules, CA, USA) using chemiluminescence (BeyoECL Plus; Beyotime Biotechnology Co., Ltd.) and analyzed using Quantity one software. The experiment was repeated in triplicate.

Statistical analysis

Statistical analysis was performed using SPSS 18.0 statistical software, and the data is expressed as the means ± SD; single factor compared analysis was used for analysis between groups, and the LSD method was used between two groups. A P-value <0.05 was considered to indicate a statistically significant difference.

Results

LoVo Cells transfected with lentivirus

LoVo cells were transfected with ART1-shRNA or ART1-cDNA lentiviral vector, and cells exhibited 80% fluorescence on the third and fifth days, respectively (Fig. 1A). ART1 expression in LoVo cells was detected by RT-PCR and western blot analysis. Compared with the untransfected and LV-control-transfected groups, ART1 mRNA and protein expression was significantly lower in the ART1-silenced groups and significantly higher in the ART1-cDNA-transfected group (P<0.05). However, there was no significant difference in ART1 mRNA and protein expression between un-transfected LoVo cells and LV-control-transfected LoVo cells (P>0.05) (Fig. 1B).

Figure 1 Lentiviral vector-mediated argenine-specific adenosine diphosphate (ADP)-ribosyltransferase 1 (ART1)-shRNA and ART1-cDNA are successfully transfected into LoVo cells. (A) LoVo cells transfected with lentiviral vector-mediated ART1-shRNA and ART1-cDNA. Ten microliters of lentiviral vector was transfected into LoVo cells, and the efficiency was observed under an optical microscope and fluorescence microscope after 96 h, when the infection rate reached 80%. (B) The expression levels of ART1 mRNA and protein in ART1-cDNA LoVo cells were compared with the ART1-shRNA LoVo cells, the lentivirus (LV)-control LoVo cells and the untransfected LoVo cells. *P<0.05 vs. untransfected LoVo cells or LV-control LoVo cells; n=3 experiments.

Effects of ART1-shRNA-transfected LoVo cells and ART1-cDNA-transfected LoVo cells on HUVEC proliferation

The proliferation capacity of HUVECs co-cultured with the supernatant fluid of ART1-shRNA LoVo cells was significantly decreased relative to the control group and untransfected group (P<0.05). By contrast, the proliferation capacity of HUVECs co-cultured with the supernatant fluid of ART1-cDNA LoVo cells was significantly higher (P<0.05), and the proliferation capacity increased with the increasing concentration of the LoVo supernatant. Furthermore, no significant difference between HUVECs co-cultured with the supernatant fluid of untransfected LoVo cells and LV-control LoVo cells was noted (P>0.05) (Fig. 2).

Figure 2 Proliferation ability of human umbilical vein endothelial cells (HUVECs) co-cultured with supernatant fluid of argenine-specific adenosine diphosphate (ADP)-ribosyltransferase 1 (ART1)-cDNA LoVo cells at ratios of 0, 20, 40, 60, 80 and 100% was compared with those co-cultured with the supernatant fluid of ART1-shRNA LoVo cells, the lentivirus (LV)-control LoVo cells and the untransfected LoVo cells. *P<0.05 vs. untransfected LoVo cells or LV-control LoVo cells; n=3 experiments.

Effects of ART1-shRNA-transfected LoVo cells and ART1-cDNA-transfected LoVo cells on HUVEC migration

Compared with the control group, the migratory ability of HUVECs co-cultured with the ART1-shRNA-transfected cells was significantly decreased (P<0.05). By contrast, HUVEC migration increased after co-culture with ART1-cDAN LoVo cells (P<0.05). The difference between HUVECs co-cultured with untransfected LoVo cells and with LV-control LoVo cells was not significant (P>0.05). Moreover, after 12 h, the majority of the HUVECs had passed through the Transwell membrane, and the difference was indistinguishable from the HUVECs co-cultured with the control LoVo cells (P>0.05) (Fig. 3).

Figure 3 The effect of argenine-specific adenosine diphosphate (ADP)-ribosyltransferase 1 (ART1)-mediated LoVo cells on HUVEC migration. (A) The migratory ability of human umbilical vein endothelial cells (HUVECs) co-cultured with ART1-mediated LoVo cells. (a) HUVECs co-cultured with ART1-shRNA LoVo cells; (b) HUVECs co-cultured with the untransfected LoVo cells; (c) HUVECs co-cultured with LV-control LoVo cells; (d) HUVECs co-cultured with the ART1-cDNA LoVo cells. (B) The comparison of migration of HUVECs co-cultured with ART1-mediated LoVo cells afer 6 and 12 h. *P<0.05 vs. untransfected LoVo cells or lentivirus (LV)-control LoVo cells; n=3 experiments.

Effects of ART1-shRNA-transfected LoVo cells and ART1-cDNA-transfected LoVo cells on the blood vessel forming ability of HUVECs

HUVEC angiogenic abilities were significantly enhanced after incubation with the supernatant of ART1-cDNA-transfected LoVo cells. Both the number and length of vessels were significantly higher than those in the control group and untransfected group (P<0.05). Furthermore, the blood vessel lumens were mutually staggered. Moreover, HUVEC vascular forming ability was significantly reduced when co-cultured with the supernatant of ART1-silenced cells (P<0.05). There was no significant difference between HUVECs incubated with the supernatant of untransfected LoVo cells and LV-control LoVo cells (P>0.05) (Fig. 4).

Figure 4 The effect of argenine-specific adenosine diphosphate (ADP)-ribosyltransferase 1 (ART1)-mediated LoVo cells on the blood vessel forming ability of human umbilical vein endothelial cells (HUVECs). (A) HUVEC angiogenic abilities when incubated with the supernatant of ART1-mediated LoVo cells. (a) HUVECs cultured with supernatant of ART1-shRNA LoVo cells; (b) HUVECs co-cultured with the untransfected LoVo cells; (c) HUVECs co-cultured with LV-control LoVo cells; (d) HUVECs co-cultured with the ART1-cDNA LoVo cells. (B) The tube length incubated with supernatant of ART1-cDNA LoVo cells was compared with incubated with the supernatant fluid of ART1-shRNA LoVo cells, the lentivirus (LV)-control LoVo cells and the untransfected LoVo cells. *P<0.05 vs. untransfected or LV-control group; n=3 experiments.

MVD in mice with transplanted tumor tissues

We noted that, compared with the control group, the MVD of tumors was significantly increased in mice which had undergone intrasplenic ART1-cDNA-transfection, whereas it was decreased in mice which had undergone intrasplenic transplantation with ART1-shRNA-transfected CT26 cells (P<0.05). However, the difference was not significant in the mice which were infected with untransfected CT26 cells and LV-control-transfected CT26 cells (P>0.05) (Fig. 5).

Figure 5 Microvessel density (MVD) in mice intrasplenic allograft tumor tissues. (A) The expression of CD34 indicated 'hot spots' and low MVD area in intrasplenic allograft tumors (H&E and immunohistochemical staining; magnification ×400). (B) The comparison of MVD in intrasplenic transplanted argenine-specific adenosine diphosphate (ADP)-ribosyltransferase 1 (ART1)-cDNA CT26 tumors with transplanted ART1-shRNA CT26 cells, LV-control CT26 cells and untransfected CT26 cells. *P<0.05 vs. untransfected or LV-control CT26 cells; n=4 mice in each group.

Effects of ART1 on the expression of t-Akt, p-Akt, HIF-1α, VEGF and bFGF

In comparison with the control and also the untransfected groups, we noted that p-Akt, HIF-1α, VEGF and bFGF expression were increased in the ART1-cDNA-transfected group, while expression was decreased in the ART1-shRNA-transfected group (P<0.05). The expression of t-Akt did not differ significantly between the experimental groups and the control groups (P>0.05). Furthermore, p-Akt, HIF-1α, VEGF and bFGF expression did not differ significantly between the untransfected LoVo cells and LV-control-transfected LoVo cells (Fig. 6).

Figure 6 Arginine-specific adenosine diphosphate (ADP)-ribosyltransferase 1 (ART1) affects total-Akt, p-Akt, HIF-1α, VEGF and bFGF expression in LoVo cells. (A) The expression of total-Akt, p-Akt, hypoxia-inducible factor 1-α (HIF-1-α), vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) in ART1-mediated LoVo cells. (B) Comparison of Akt, p-Akt, HIF-1α, VEGF and bFGF expression in ART1-cDNA-transfected LoVo cells with that of ART1-shRNA-transfected LoVo cells, LV-control-transfected LoVo cells and untransfected LoVo cells. *P<0.05 vs. untransfected LoVo cells; n=3 experiments.

Discussion

ART1, a representative ecto-ART family protein, catalyzes ADP-ribosylation, which transfers an ADP-ribose from NAD+ to an amino acid residue, and thus plays an important role in the protein modification mechanism. Pioneering research has found that certain growth factors are directly regulated by ART1, and ART1 modulates the binding of PDGF-BB to its receptor and regulates its signal transduction capabilities (12). Our earlier studies have demonstrated that ART1 is related to apoptosis, proliferation and migration of mouse CT26 cells (28–30), suggesting that ART1 is involved in tumor growth, invasion and metastasis. It is widely known that angiogenesis plays an important role in malignant tumor occurrence, development, invasion and metastasis, but as yet there have been no studies regarding the role of ART1 in angiogenesis during CRC.

We found that ART1 was highly expressed in a human CRC and was positively correlated with the expression of antigenic factors (19). In order to further confirm the association between ART1 and angiogenesis, in the present study, ART1 was stably expressed at a high level in the LoVo cell line and silenced using lentiviral vectors. Additionally, LoVo cells were co-cultured with HUVECs to determine the effects of high expression of ART1 or ART1 silencing on HUVEC proliferation, migration and angiogenesis. Our data demonstrated that in LoVo cells with high ART1 expression, HUVEC proliferation, migration and angiogenesis was increased relative to the control. By contrast, HUVEC proliferation, migration and angiogenesis were significantly reduced following co-culture with ART1-silenced LoVo cells. These data suggest that ART1 promotes the proliferation and migration of vascular endothelial cells, thereby inducing tumor angiogenesis.

VEGF is a crucial mitogen for vascular endothelial cells in vitro and in vivo, and plays an important role in inducing endothelial cell proliferation and angiogenesis. VEGF has been shown to correlate with angiogenesis in a number of human cancers, as evidenced by MVD (31,32). bFGF is the strongest growth factor to induce cell generation, and increases the expression of VEGF in endothelial cells while directly contributing to the proliferation of vascular endothelial cells, exerting a synergistic effect with VEGF (33). VEGF/bFGF-induced angiogenesis is a crucial step in tumor progression and metastasis. The data in our present study showed that compared with the control lentivirus-transfected group and untransfected group, VEGF and bFGF expression was upregulated in the group transfected with ART1-cDNA, while expression was downregulated in silenced ART1 LoVo cells. MVD was significantly higher in tumor tissues of the mice which had been infected with CT26 cells transfected with ART1-cDNA, whereas angiogenesis was inhibited when ART1 was silenced in the intrasplenic implantation model. These results suggest that ART1 promotes the expression of VEGF and bFGF, but its mechanism is not yet clear.

Our previous research has shown that the ART1-specific inhibitor MIBG inhibits the expression of the integrin α7β1, focal adhesion kinase (FAK) and PI3K, thereby inhibiting HepG2 cell growth and invasion (34). Another study has shown that ARTs directly regulate phosphodiesterase in the retina (35), suggesting that ARTs affects protein functions by directly regulating ADP-ribosylation, thus modulating signal transduction. It has previously been deonstrated that multiple signaling networks are involved in the regulation of tumor angiogenesis, and the PI3K/Akt pathway has been shown to be of significant importance (36).

Akt is a major downstream effector molecule of PI3K (37), and increased AktThr308 phosphorylation implies the augmentation of the PI3K pathway (38); it can directly phosphorylate various transcription factors such as NF-κB and mTOR and is involved in the regulation of various biological activities (39). PI3K activation induces Akt phosphorylation, thus promoting GSK-3 phosphorylation, inhibiting HIF-1α degradation (40), activating the downstream gene mTOR and promoting HIF-1α gene transcription (41). HIF-1α has been revealed to play a central role in regulating the metabolic switch in cancer (42). Overexpression of HIF-1α is related to increased mortality in patients with various types of tumor (43,44) HIF-1α is activated by PI3K-Akt-mTOR pathways and hyper-activity of RAS/MAPK (45). It is also activated by oncogenic mutation of PTEN and VHL (46,47). Activated HIF-1α binds to the hypoxia response element (HREs), promoting the transcription of a variety of pro-angiogenic genes in the cell and cellular matrix and upregulating VEGF, bFGF expression and that of other angiogenesis-related factors in tumor tissues (48). HIF-1α regulates the expression of VEGF in cancer cells, is activated by growth factors, and induces formation of the proangiogenic microenvironment in gastric cancer (45). The CD31-positive vessels and tumor growth both decreased in a subcutaneous implantation model when HIF-1α activation was blocked in a gastric cancer cell line (49). These results suggest that suppression of Thr PI3K/Akt pathway leads to decreased expression of HIF-1α and inhibits tumor angiogenesis.

Based on these studies, in the present study we detected the expression of t-Akt, p-Akt and HIF-1α, and the data showed that compared with the control groups, p-Akt, and HIF-1α expression was upregulated in LoVo cells transfected with ART1-cDNA, whereas expression was downregulated in LoVo cells transfected with ART1-shRNA. We speculate that ART1 regulates HIF-1α expression through the PI3K/Akt pathway. Similar to the effects of ART1 silencing, the PI3K/Akt pathway inhibitor LY294002 inhibited Akt phosphorylation but failed to bring about significant changes in ART1 expression (28), and this confirms that the Akt pathway is located downstream of ART1. These data suggest that ART1 promotes Akt phosphorylation and subsequent HIF-1α expression and activation, increases VEGF and bFGF expression of cancer cells, and eventually leads to an increase in tumor angiogenesis in tumor tissue.

In brief, these data suggest that the mechanism of ART1 in tumor angiogenesis is quite complex and is involved in multiple signaling pathways. The data from the present study demonstrate that ART1 upregulates HIF-1α through the PI3K/Akt signaling pathway to promote the expression of angiogenic factors such as VEGF and bFGF and induce angiogenesis in cancer tissue. Furthermore, its exact mechanisms require further in-depth study.

Acknowledgments

This study was supported by the Ministry of Education Specialized Research Fund for the Doctoral Program of Higher Education (Grant no. 20105503110009), the Science and Technology Project of Chongqing Municipal Education Commission (Grant no. KJ110322) and the National Nature Science Foundation of China (NSFC: 30870946). We would like to thank Tang Yi and Xiao Ming for preparing the CT26 murine CRC cells, and we also thank Kuang Jing, and Song Guang-Lin, postgraduate students of Chongqing Medical and Science University, for their assistance in this study.

References