Downregulation of miR-10b promotes osteoblast differentiation through targeting Bcl6

  • Authors:
    • Jizhou Yang
    • Shaojie Wang
    • Fengxian Wang
    • Xiaohong Mu
    • Yi Qu
    • Ziyi Zhao
    • Xing Yu
  • View Affiliations

  • Published online on: April 20, 2017     https://doi.org/10.3892/ijmm.2017.2955
  • Pages:1605-1612
0

Abstract

MicroRNAs (miRNAs or miRs) have been shown to play a critical role in osteoblast differentiation. miR-10b has been found to be downregulated during osteoblast differentiation; however, its precise effect on osteoblast differentiation remains unknown. In this study, we aimed to investigate the potential role of miR-10b and the potential underlying mechanism in regulating osteoblast differentiation. We found that miR-10b was downregulated during osteoblast differentiation. Overexpression of miR-10b inhibited osteoblast differentiation, whereas the suppression of miR-10b promoted osteoblast differentiation. Bioinformatics analysis and the dual-luciferase reporter assay demonstrated that miR-10b could target the 3'-untranslated regions of B cell lymphoma 6 (Bcl6) which is an important regulator of osteoblast differentiation. Real‑time quantitative polymerase chain reaction and western blot analysis showed that miR-10b directly regulated Bcl6 expression. Further experiments showed that the overexpression of miR-10b increased the expression of signal transducer and activator of transcription 1 (STAT1) and blocked Runt-related transcription factor 2 (Runx2) nuclear translocation, whereas miR-10b suppression showed an opposite effect. Moreover, the miR-10b suppression-induced effects were partially reversed by Bcl6 knockdown. Taken together, our study suggests that miR-10b contributes to osteoblast differentiation through targeting Bcl6, providing a novel insight into understanding the molecular mechanism underlying osteoblast differentiation and suggesting a potential target for inhibiting bone loss.

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June 2017
Volume 39 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

2016 Impact Factor: 2.341
Ranked #21/128 Medicine Research and Experimental
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APA
Yang, J., Wang, S., Wang, F., Mu, X., Qu, Y., Zhao, Z., & Yu, X. (2017). Downregulation of miR-10b promotes osteoblast differentiation through targeting Bcl6. International Journal of Molecular Medicine, 39, 1605-1612. https://doi.org/10.3892/ijmm.2017.2955
MLA
Yang, J., Wang, S., Wang, F., Mu, X., Qu, Y., Zhao, Z., Yu, X."Downregulation of miR-10b promotes osteoblast differentiation through targeting Bcl6". International Journal of Molecular Medicine 39.6 (2017): 1605-1612.
Chicago
Yang, J., Wang, S., Wang, F., Mu, X., Qu, Y., Zhao, Z., Yu, X."Downregulation of miR-10b promotes osteoblast differentiation through targeting Bcl6". International Journal of Molecular Medicine 39, no. 6 (2017): 1605-1612. https://doi.org/10.3892/ijmm.2017.2955