Open Access

G-protein-coupled receptor 30 mediates the effects of estrogen on endothelial cell tube formation in vitro

  • Authors:
    • Liyuan Zhou
    • Hong Chen
    • Xun Mao
    • Hongbo Qi
    • Philip N. Baker
    • Hua Zhang
  • View Affiliations

  • Published online on: April 20, 2017     https://doi.org/10.3892/ijmm.2017.2957
0

Abstract

The placenta is the exchange organ between the mother and the fetus. The inadequate function of this organ is associated with a number of pregnancy disorders. Hypoxia and oxidative stress during placental development may induce endothelial dysfunction, resulting in the reduction in the perfusion of the placenta. During pregnancy, the levels of estrogen are increased. Decreased estrogen levels have been reported in women with preeclampsia. However, whether estrogen is involved in placental angiogenesis remains unclear. In this study, we aimed to investigate the effects of estrogen on endothelial cell tube formation and to elucidate the underlying mechanisms. For this purpose, human umbilical vein endothelial cells (HUVECs) were cultured with 17‑β‑estradiol under conditions of hypoxia/reoxygenation (H/R). The total pipe length of the tube‑like structure on endothelial cells was measured. The expression levels of G‑protein‑coupled receptor 30 (GPR30) and endothelial nitric oxide synthase (eNOS) and Akt were also measured in the endothelial cells following treatment with 17‑β‑estradiol under H/R conditions by western blot analysis and immunostaining. We found that the total pipe length of the tube‑like structure on endothelial cells was significantly reduced. This reduction was reversed by treatment with 17‑β‑estradiol. The expression of GPR30 in endothelial cells was significantly increased following treatment with 17‑β‑estradiol under H/R conditions. Furthermore, the levels of eNOS and Akt in endothelial cells were also significantly increased following treatment with 17-β-estradiol under H/R conditions. The activation of eNOS was inhibited by wortmannin, an inhibitor of PI3K/Akt. Our data thus demonstrate that estrogen prevents the failure of endothelial cell tube formation induced by H/R. GPR30 plays an important role in these protective effects through the activation of eNOS and Akt in endothelial cells. Our data suggest that increased levels of estrogen are important for placental angiogenesis.

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Print ISSN: 1107-3756
Online ISSN:1791-244X

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APA
Zhou, L., Chen, H., Mao, X., Qi, H., Baker, P.N., & Zhang, H. (1899). G-protein-coupled receptor 30 mediates the effects of estrogen on endothelial cell tube formation in vitro. International Journal of Molecular Medicine, 0, 0-0. https://doi.org/10.3892/ijmm.2017.2957
MLA
Zhou, L., Chen, H., Mao, X., Qi, H., Baker, P. N., Zhang, H."G-protein-coupled receptor 30 mediates the effects of estrogen on endothelial cell tube formation in vitro". International Journal of Molecular Medicine 0.0 (1899): 0-0.
Chicago
Zhou, L., Chen, H., Mao, X., Qi, H., Baker, P. N., Zhang, H."G-protein-coupled receptor 30 mediates the effects of estrogen on endothelial cell tube formation in vitro". International Journal of Molecular Medicine 0, no. 0 (1899): 0-0. https://doi.org/10.3892/ijmm.2017.2957