Anti-inhibitory potential of an ethanolic extract of Distromium decumbens on pro-inflammatory cytokine production in Pseudomonas aeruginosa lipopolysaccharide-stimulated nasal polyp-derived fibroblasts

  • Authors:
    • Dae-Sung Lee
    • Chang-Min Lee
    • Seong Kook Park
    • Mi-Jin Yim
    • Jeong Min Lee
    • Grace Choi
    • Jong Su Yoo
    • Won-Kyo Jung
    • Saegwang Park
    • Su-Kil Seo
    • Won Sun Park
    • Il-Whan Choi
  • View Affiliations

  • Published online on: October 11, 2017     https://doi.org/10.3892/ijmm.2017.3182
  • Pages:1950-1956
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Abstract

Marine algae are rich sources of biologically active compounds that may present useful leads in the development of pharmaceuticals, nutraceuticals, and functional foods. The main aim of this study was to identify the possible anti-inflammatory effects of Distromium decumbens in nasal polyp-derived fibroblasts (NPDFs) and its associated mechanism of action. NPDFs were stimulated by Pseudomonas aeruginosa lipopolysaccharide (PA-LPS) and treated with an ethanolic extract of Distromium decumbens (DDE). The production of interleukin-6 (IL-6) and IL-8 in the supernatant, the phosphorylation of mitogen-activated protein kinase (MAPK) molecules [extracellular signal-related kinase 1/2 (ERK1/2), c-Jun N-terminal kinase and p38 MAPK] and Akt, and the activation of nuclear factor-κB (NF-κB) were assayed in the PA-LPS-stimulated NPDFs untreated or treated with DDE. The expression levels of IL-6 and IL-8 in PA-LPS-exposed NPDFs were detected using enzyme-linked immunosorbent assays. The mechanisms by which DDE regulates cellular signaling cascades were investigated using electrophoretic mobility shift assays and western blot analysis. Functional validation was performed by measuring the inhibitory effects of DDE on neutrophil migration in vitro. DDE reduced the expression of IL-6 and IL-8 stimulated by PA-LPS in NPDFs. The activation of ERK1/2, Akt and NF-κB by PA-LPS was inhibited by DDE. Inhibitors of ERK1/2, Akt and NF-κB inhibited the expression of IL-6 and IL-8. In addition, DDE significantly attenuated PA-LPS-induced migration of differentiated HL-60 cells. The present findings suggest that DDE potently inhibits inflammation through the ERK1/2, Akt and NF-κB signaling pathways in NPDFs.

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December 2017
Volume 40 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

2016 Impact Factor: 2.341
Ranked #21/128 Medicine Research and Experimental
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APA
Lee, D., Lee, C., Park, S.K., Yim, M., Lee, J.M., Choi, G. ... Choi, I. (2017). Anti-inhibitory potential of an ethanolic extract of Distromium decumbens on pro-inflammatory cytokine production in Pseudomonas aeruginosa lipopolysaccharide-stimulated nasal polyp-derived fibroblasts. International Journal of Molecular Medicine, 40, 1950-1956. https://doi.org/10.3892/ijmm.2017.3182
MLA
Lee, D., Lee, C., Park, S. K., Yim, M., Lee, J. M., Choi, G., Yoo, J. S., Jung, W., Park, S., Seo, S., Park, W. S., Choi, I."Anti-inhibitory potential of an ethanolic extract of Distromium decumbens on pro-inflammatory cytokine production in Pseudomonas aeruginosa lipopolysaccharide-stimulated nasal polyp-derived fibroblasts". International Journal of Molecular Medicine 40.6 (2017): 1950-1956.
Chicago
Lee, D., Lee, C., Park, S. K., Yim, M., Lee, J. M., Choi, G., Yoo, J. S., Jung, W., Park, S., Seo, S., Park, W. S., Choi, I."Anti-inhibitory potential of an ethanolic extract of Distromium decumbens on pro-inflammatory cytokine production in Pseudomonas aeruginosa lipopolysaccharide-stimulated nasal polyp-derived fibroblasts". International Journal of Molecular Medicine 40, no. 6 (2017): 1950-1956. https://doi.org/10.3892/ijmm.2017.3182