The enhancement of amyloid precursor protein and β-site amyloid cleavage enzyme 1 interaction: Amyloid-β production with aging

  • Authors:
    • Lin Zou
    • Rongxi Yang
    • Penghui Zhang
    • Yi Dai
  • View Affiliations

  • Published online on: March 1, 2010     https://doi.org/10.3892/ijmm_00000358
  • Pages: 401-407
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Aging is considered a high risk factor for Alzheimer's disease (AD), which is one of the most prevalent neurodegenerative disorders in the elderly population. The major pathologic feature of AD is senile plaques mainly containing amyloid-β (Aβ) components. However, little direct evidence has shown aging in association with Aβ. Here we show that the protein-protein interaction of amyloid precursor protein (APP) and β -site amyloid cleavage enzyme 1 (BACE1) is enhanced by the fluorescence resonance energy transfer (FRET) assay during the aging process, and the APP-BACE1 complex accumulates in the endosome in the IMR-90 fibroblast (NHF) cellular aging models. Moreover, enhanced Aβ is observed in aged cells, rat brain homogenates and human serum. Interestingly, addition of the dominant-negative mutant of Rab5, a small G-protein Rab5 involved in the endocytic process, inhibits the aging-related APP-BACE1 interaction and Aβ production, suggesting that endocytosis contributes to AD progression.

Related Articles

Journal Cover

March 2010
Volume 25 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Zou L, Yang R, Zhang P and Dai Y: The enhancement of amyloid precursor protein and β-site amyloid cleavage enzyme 1 interaction: Amyloid-β production with aging. Int J Mol Med 25: 401-407, 2010.
APA
Zou, L., Yang, R., Zhang, P., & Dai, Y. (2010). The enhancement of amyloid precursor protein and β-site amyloid cleavage enzyme 1 interaction: Amyloid-β production with aging. International Journal of Molecular Medicine, 25, 401-407. https://doi.org/10.3892/ijmm_00000358
MLA
Zou, L., Yang, R., Zhang, P., Dai, Y."The enhancement of amyloid precursor protein and β-site amyloid cleavage enzyme 1 interaction: Amyloid-β production with aging". International Journal of Molecular Medicine 25.3 (2010): 401-407.
Chicago
Zou, L., Yang, R., Zhang, P., Dai, Y."The enhancement of amyloid precursor protein and β-site amyloid cleavage enzyme 1 interaction: Amyloid-β production with aging". International Journal of Molecular Medicine 25, no. 3 (2010): 401-407. https://doi.org/10.3892/ijmm_00000358